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Electrochemical immunosensor for determination of Staphylococcus aureus bacteria by IgY immobilized on glassy carbon electrode with electrodeposited gold nanoparticles

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Abstract

A new ultrasensitive immunosensor is proposed based on the covalently attached anti-protein A antibody (IgY) on deposited gold nanoparticle (AuNP)-modified glassy carbon electrode (GCE) for the electrochemical measurement of Staphylococcus aureus (S. aureus). Chicken IgY as a capture antibody provides highly selective and specific binding to the target bacteria and selectively captures the S. aureus in its three-dimensional space. Due to that it can eliminate the interference from protein G-producing Streptococcus. In addition, the electron-transfer characteristic of [Fe(CN)6]4−/3− is hindered by this combination; as it is reflected on the electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) curves. The proposed immunosensor displays a wide linear dynamic range from 10 to 107 CFU mL−1 with a detection limit of 3.3 CFU mL−1 with RSD 3.0%. It is capable to accurately determine S. aureus in milk and human blood serum as a complex matrix sample with satisfactory recovery of ∼ 97–103%. The immunosensor also displays high selectivity over other bacteria and acceptable stability. Presumably, our study can be regarded as the first one to report chicken IgY in order to detect S. aureus based on an electrochemical method.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Sciences, Ilam University, PO Box 69315-516, Ilam, Iran

    Mahmoud Roushani & Zeinab Rahmati

  2. Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

    Mehdi Golchin & Zinat Lotfi

  3. Department of Veterinary, Faculty of microbiology, Ilam University, PO Box 69315-516, Ilam, Iran

    Mostafa Nemati

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  1. Mahmoud Roushani
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  2. Zeinab Rahmati
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  3. Mehdi Golchin
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  4. Zinat Lotfi
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  5. Mostafa Nemati
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Correspondence to Mahmoud Roushani.

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All experimental protocols were approved by the local Animal Experimentation Ethics Committee of Kerman University (Code: IR. UK. VETMED. REC.1399.008). The human blood serum samples were provided from a local clinical laboratory. Informed consent was obtained from all participants included in the study.

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Roushani, M., Rahmati, Z., Golchin, M. et al. Electrochemical immunosensor for determination of Staphylococcus aureus bacteria by IgY immobilized on glassy carbon electrode with electrodeposited gold nanoparticles. Microchim Acta 187, 567 (2020). https://doi.org/10.1007/s00604-020-04547-6

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