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An electrochemical peptide-based biosensor for the Alzheimer biomarker amyloid-β(1–42) using a microporous gold nanostructure

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Abstract

Alzheimer’s disease (AD) is connected to aggregation of amyloid-β (Aβ) peptide and formation of insoluble plaques in the brain. Aβ level can be monitored as an AD early diagnosis route. In this study, an irregular shaped microporous gold nanostructure with a typical size of 150 × 250 nm was electrodeposited on a polycrystalline gold surface at 0 mV (vs. AgCl) using sodium alendronate. The nanostructure was then characterized by field-emission scanning electron microscopy. An electrochemical peptide-based biosensor was fabricated by immobilizing an Aβ(1–42)-binding peptide on the gold nanostructure. Binding of Aβ(1–42) by the peptide was followed electrochemically using ferro/ferricyanide as a redox probe. Differential pulse voltammograms in a potential range of 0–500 mV (vs. AgCl) with typical peak potentials at 224 mV are linear in the 3–7000 pg mL−1 Aβ(1–42) concentration range, with a 0.2 pg mL−1 detection limit. The biosensor is free of interferences and was applied to the quantitation of Aβ(1–42) in artificial cerebrospinal fluid and spiked serum samples.

Schematic presentation of an immobilized amyloid-β(1-42)-specific peptide on the surface of a microporous gold nanostructure to fabricate an electrochemical biosensor for early diagnosis of Alzheimer’s disease. Aβ(1-42) capturing by the peptide led to repulsion of ferrocyanide/ferricyanide redox couple.

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Acknowledgments

The financial support of the Iran National Science Foundation Grant NO. 96005985 is gratefully acknowledged. We would also like to thank the Research Councils of Shiraz University of Medical Sciences.

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  1. Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, 7193635899, Iran

    Masoud Negahdary & Hossein Heli

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  1. Masoud Negahdary
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  2. Hossein Heli
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Correspondence to Hossein Heli.

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Negahdary, M., Heli, H. An electrochemical peptide-based biosensor for the Alzheimer biomarker amyloid-β(1–42) using a microporous gold nanostructure. Microchim Acta 186, 766 (2019). https://doi.org/10.1007/s00604-019-3903-x

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