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Fluorometric microplate-based dimethoate assay using CdSe/ZnS quantum dots coated with a molecularly imprinted polymer

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Abstract

A microplate-based assay is described for the sensitive and selective fluorometric determination of the pesticide dimethoate. Molecularly imprinted polymer (MIP)-coated CdSe/ZnS quantum dots (QDs) are used as the molecular recognition probe. The MIP-coated QDs were synthesized using one-step reversed-phase microemulsion in the presence of hydrophobic CdSe/ZnS QDs. Copolymerization was performed by using 3-aminopropyltriethoxysilane as the functional monomer, tetraethoxysilane as the cross-linker, and dimethoate as the template. The fluorescence of the coated QDs is quenched by dimethoate when dimethoate becomes rebound on the imprinting sites of the MIP. Under the optimal conditions, fluorescence (best measured at excitation/emisison wavelengths of 380/620 nm) drops linearly in the 5.0–150 μg L−1 dimethoate concentration range, and the limit of detection is 2.1 μg L−1 (at S/N = 3). The assay was utilized for dimethoate determination in spiked real samples. Satisfactory recoveries (89.8%–98.0%) with relatively standard deviations of <4.9% are obtained. The method is rapid, cost-effective, sensitive, and selective. The use of microplate allows for the quantitation of a large number of samples simultaneously.

Schematic representation of sensitive and selective fluorometric microplate-based assay for the high-throughput determination of dimethoate (DM) based on recognition of molecularly imprinted polymer (MIP)-coated CdSe/ZnS quantum dots (QDs). DM exerts a quenching effect on the fluorescence of the QDs.

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Acknowledgments

Special thanks to the Scientific Instrument Centre (Shanxi University), Wei Zhou and Zhiqiang Guo for the convenience of the characterization experiment. This work was supported by the National Natural Science Foundation of China (project No. 31701686, 31801670); the Young Technology Research Fund of Shanxi, China (project No. 201701D221180); Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi (project No. 201802016); the fund of the Beijing Laboratory for Food Quality and Safety, Beijing Technology and Business University (project No. FQS-201803).

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Authors and Affiliations

  1. School of Life Science, Shanxi University, No. 92, Wucheng Road, Taiyuan, 030006, China

    Yukun Yang, Yuanyuan Chang, Yuanyuan Guo, Ligang Yu, Guohua Zhang & Doudou Zhai

  2. Beijing Laboratory for Food Quality and Safety, Beijing Technology and Business University, Beijing, 100048, China

    Yukun Yang & Xiaotao Sun

  3. College of Pharmaceutical and Food Engineering, Shanxi University of Chinese Medicine, Jinzhong, 030619, China

    Xiaomin Wang

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  1. Yukun Yang
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Correspondence to Yukun Yang, Xiaomin Wang or Xiaotao Sun.

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Yang, Y., Chang, Y., Guo, Y. et al. Fluorometric microplate-based dimethoate assay using CdSe/ZnS quantum dots coated with a molecularly imprinted polymer. Microchim Acta 186, 589 (2019). https://doi.org/10.1007/s00604-019-3649-5

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