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Fluorometric detection of silver(I) using cytosine-Ag(I)-cytosine pair formation, DNA assembly and the AND logic operation of a multiple-component DNAzyme

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Abstract

A nonenzymatic fluorometric assay is described for highly sensitive and selective detection of silver ion. It is making use of a controlled DNA assembly and an AND logic operation of a multiple-component DNAzyme (MNAzyme). It corresponds to an Ag(I)-responsive three-way junction (3-WJ) assembly. The tailored probes of the 3-WJ architecture were designed with complementary domains for subsequent assembly. Cytosine (C)-cytosine mismatches at one-way junction were set as the sensing element for Ag(I). Upon exposure to Ag(I) as an input, C-Ag(I)-C pairs are being formed. This enhances the binding energy between these separate probes and thus promotes the formation of a nanostructure that represents an AND logic assembly of MNAzyme with an amplified output signal. This results in an Ag(I)-induced increase in fluorescence which is measured best at excitation/emission maxima of 645/670 nm. The method displays high selectivity and sensitivity, has a 5 pM detection limit at 3σ and a dynamic range that extends from 10 pM to 100 nM.

Schematic presentation of a new fluorescence system for determining silver ion by making use of cytosine-Ag(I)-cytosine pair formation, precisely-controlled DNA assembly and “AND” logic operation of multiple components DNAzyme (MNAzyme).

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Acknowledgements

This work was funded by Wanzhou District Science and Technology Project (wzstc-2018008), Ph.D. Programs Foundation of Chongqing Three Gorges Central Hospital (2017BSKYQDJJ06), National Natural Science Foundation of China (81860380) and Fund of Guizhou Provincial Chinese Medicine Administration (QZYY-2018-084); special thanks for the advice from Lan Lin and Yong Luo on our manuscript.

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Author notes

  1. Yongjie Xu and Cheng Zhang contributed equally to this work.

Authors and Affiliations

  1. Department of Laboratory Medicine, Guizhou Provincial People′s Hospital, Guiyang, 550002, China

    Yongjie Xu, Qin Li & Hua Zhang

  2. Department of Endocrinology, Chongqing Three Gorges Central Hospital, Chongqing, 404000, China

    Cheng Zhang & Xiaohe Luo

  3. Department of Laboratory Medicine, Chongqing Three Gorges Central Hospital, Chongqing, 404000, China

    Hongxin Du & Xiaohe Luo

Authors
  1. Yongjie Xu
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  2. Cheng Zhang
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  3. Hongxin Du
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  4. Qin Li
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  5. Hua Zhang
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  6. Xiaohe Luo
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Correspondence to Yongjie Xu, Hua Zhang or Xiaohe Luo.

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Xu, Y., Zhang, C., Du, H. et al. Fluorometric detection of silver(I) using cytosine-Ag(I)-cytosine pair formation, DNA assembly and the AND logic operation of a multiple-component DNAzyme. Microchim Acta 186, 522 (2019). https://doi.org/10.1007/s00604-019-3615-2

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  • DOI: https://doi.org/10.1007/s00604-019-3615-2

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