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Two-dimensional MoS2 as a nano-binder for ssDNA: Ultrasensitive aptamer based amperometric detection of Ochratoxin A

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Abstract

Two-dimensional (2D) MoS2 is found to possess different affinities for ssDNA and dsDNA. This finding is exploited in an amperometric aptamer-based method for the determination of the mycotoxin ochratoxin A (OTA). Initially, a dsDNA probe (formatted through the hybridization of OTA-aptamer with an auxiliary DNA) is self-assembled on a gold electrode. Upon introduction of OTA, it will bind to the aptamer and cause the unwinding of dsDNA, while the auxiliary DNA (with single-stranded structure) remains on the electrode. Since the affinity of 2D MoS2 for ssDNA is considerably larger than that for dsDNA, it will be adsorbed on the electrode by binding to the auxiliary DNA. Notably, 2D MoS2 possesses peroxidase-like activity. Hence, it can catalyze the amplification of electrochemical signal of the hydroquinone/benzoquinone redox system. Under optimal conditions, the amperometric signal (best measured at −0.2 V vs. SCE) increases with increasing OTA concentration in the range from 0.5 pg·mL−1 to 1.0 ng·mL−1, with a lower detection limit of 0.23 pg·mL−1. The method was applied to the determination of OTA in spiked red wine.

Herein we construct a convenient electrochemical aptasensor for sensitive monitor of ochratoxin A by using 2D MoS2 as a nano-binder to catalyze the amplification of electrochemical signal from hydroquinone/benzoquinone system.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (21505060), the Foundation of Jiangxi Educational Committee (GJJ150327), the Science Foundation of Jiangxi Province (20161BAB213073), Scientific Research Foundation of Jiangxi Normal University, the Open Project Program of Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Jiangxi Normal University (No. KLFS-KF-201710), Outstanding Youth Funds of Jiangxi Normal University and the Advanced Research Fund of Quanzhou Normal University for Young Doctor (2016QBKJ03).

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Authors and Affiliations

  1. Ministry of Education Key Laboratory of Functional Small Organic Molecule, College of Chemistry and chemical engineering, Jiangxi Normal University, Nanchang, 330022, People’s Republic of China

    Juan Tang, Yapei Huang, Yu Cheng & Lulu Huang

  2. College of Chemical Engineering and Materials Science, Quanzhou Normal University, Quanzhou, 362000, Fujian Province, People’s Republic of China

    Junyang Zhuang

  3. Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, 350108, People’s Republic of China

    Dianping Tang

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Correspondence to Juan Tang.

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Tang, J., Huang, Y., Cheng, Y. et al. Two-dimensional MoS2 as a nano-binder for ssDNA: Ultrasensitive aptamer based amperometric detection of Ochratoxin A. Microchim Acta 185, 162 (2018). https://doi.org/10.1007/s00604-018-2706-9

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