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Aptamer based fluorometric determination of kanamycin using double-stranded DNA and carbon nanotubes

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Abstract

The authors describe a fluorometric assay for the antibiotic kanamycin that is making use of a fluorescein-labeled aptamer against kanamycin and carbon nanotubes (CNTs). It is found that CNTs do not quench the fluorescence of the duplex formed between FAM-labeled aptamer and its complementary DNA (cDNA). However, if kanamycin is added, it will bind to the aptamer, and this leads to dehybridization of the duplex upon which the fluorescence of the label is quenched by the CNTs present in solution. The method has an analytical range that extends from 1.0 to 50 nmol L-1 of kanamycin, and the detection limit is 0.4 nmol L−1. It was successfully employed to the detection of kanamycin in (spiked) milk samples and gave good recoveries.

The fluorescent detection platform uses double-stranded DNA and carbon nanotubes, is simple, and has a detection limit as low as 0.4 nmol L−1.

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Acknowledgements

This work was supported by the fund from innovation fund of Jiangxi academy of agricultural sciences (No. 2014CQN010) and National quality and safety risk assessment of livestock and poultry products in 2016(No. GJFP2016007).

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Authors and Affiliations

  1. Laboratory of Quality & Safety Risk Assessment of Livestock and Poultry Products (Nanchang), Ministry of Agriculture, Agricultural Product Quality Safety and Standards Institute, Jiangxi Academy of Agricultural Sciences, Nanchang, 330200, People’s Republic of China

    Qie Gen Liao, Ben Hua Wei & Lin Guang Luo

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  1. Qie Gen Liao
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  2. Ben Hua Wei
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  3. Lin Guang Luo
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Correspondence to Lin Guang Luo.

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Liao, Q.G., Wei, B.H. & Luo, L.G. Aptamer based fluorometric determination of kanamycin using double-stranded DNA and carbon nanotubes. Microchim Acta 184, 627–632 (2017). https://doi.org/10.1007/s00604-016-2050-x

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  • DOI: https://doi.org/10.1007/s00604-016-2050-x

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