Skip to main content

Advertisement

SpringerLink
Log in

Long-term outcomes of patch tracheoplasty using collagenous tissue membranes (biosheets) produced by in-body tissue architecture in a beagle model

  • Original Article
  • Published:
Surgery Today Aims and scope Submit manuscript

Abstract

Purpose

Although various artificial tracheas have been developed, none have proven satisfactory for clinical use. In-body tissue architecture (IBTA) has enabled us to produce collagenous tissues with a wide range of shapes and sizes to meet the needs of individual recipients. In the present study, we investigated the long-term outcomes of patch tracheoplasty using an IBTA-induced collagenous tissue membrane (“biosheet”) in a beagle model.

Methods

Nine adult female beagles were used. Biosheets were prepared by embedding cylindrical molds assembled with a silicone rod and a slitting pipe into dorsal subcutaneous pouches for 2 months. The sheets were then implanted by patch tracheoplasty. An endoscopic evaluation was performed after 1, 3, or 12 months. The implanted biosheets were harvested for a histological evaluation at the same time points.

Results

All animals survived the study. At 1 month after tracheoplasty, the anastomotic parts and internal surface of the biosheets were smooth with ciliated columnar epithelium, which regenerated into the internal surface of the biosheet. The chronological spread of chondrocytes into the biosheet was observed at 3 and 12 months.

Conclusions

Biosheets showed excellent performance as a scaffold for trachea regeneration with complete luminal epithelium and partial chondrocytes in a 1-year beagle implantation model of patch tracheoplasty.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Ott LM, Vu CH, Farris AL, Fox KD, Galbraith RA, Weiss ML, et al. Functional reconstruction of tracheal defects by protein-loaded, cell-seeded, fibrous constructs in rabbits. Tissue Eng Part A. 2015;21:2390–403.

    Article  CAS  Google Scholar 

  2. Nakayama Y, Ishibashi-Ueda H, Takamizawa K. In vivo tissue-engineered small-caliber arterial graft prosthesis consisting of autologous tissue (biotube). Cell Transpl. 2004;13:439–49.

    Article  Google Scholar 

  3. Yamanami M, Ishibashi-Ueda H, Yamamoto A, Iida H, Watanabe T, Kanda K, et al. Transplantation study of small-caliber ‘‘biotube’’ vascular grafts in a rat model. J Artif Organs. 2013;16:59–655.

    Article  CAS  Google Scholar 

  4. Watanabe T, Kanda K, Ishibashi-Ueda H, Yaku H, Nakayama Y. Autologous small-caliber “biotube” vascular grafts with argatroban loading: a histomorphological examination after transplantation to rabbits. J Biomed Mater Res B Appl Biomater. 2010;92:236–42.

    Article  Google Scholar 

  5. Okuyama H, Umeda S, Takama Y, Terasawa T, Nakayama Y. Patch esophagoplasty using an in-body-tissue-engineered collagenous connective tissue membrane. J Pediatr Surg. 2017;53:223–6.

    Article  Google Scholar 

  6. Satake R, Komura M, Komura H, Kodaka T, Terawaki K, Ikebukuro K, et al. Patch tracheoplasty in body tissue engineering using collagenous connective tissue membranes (biosheets). J Pediatr Surg. 2013;51:244–8.

    Article  Google Scholar 

  7. Jungebluth P, Haag JC, Sjöqvist S, Gustafsson Y, Beltrán Rodríguez A, Del Gaudio C, et al. Tracheal tissue engineering in rats. Nat Protoc. 2014;9:2164–79.

    Article  CAS  Google Scholar 

  8. Delaere PR, Raemdonck DV. The trachea: the first tissue-engineered organ? J Thorac Cardiovasc Surg. 2014;147:1128–32.

    Article  Google Scholar 

  9. Grillo HC. Tracheal replacement: a critical review. Ann Thorac Surg. 2002;73:1995–2004.

    Article  Google Scholar 

  10. Tsugawa C, Nishijima E, Muraji T, Satoh S, Takamizawa S, Yamaguchi M, et al. Tracheoplasty for long segment congenital tracheal stenosis: analysis of 29 patients over two decades. J Pediatr Surg. 2003;38:1703–6.

    Article  Google Scholar 

  11. Oue T, Kamata S, Usui N, Okuyama H, Nose K, Okada A. Histopathologic changes after tracheobronchial reconstruction with costal cartilage graft for congenital tracheal stenosis. J Pediatr Surg. 2001;36:329–33.

    Article  CAS  Google Scholar 

  12. Yazdanbakhsh AP, van Rijssen LB, Koolbergen DR, König A, de Mol BA, Hazekamp MG. Long-term follow-up of tracheoplasty using autologous pericardial patch and strips of costal cartilage. Eur J Cardiothorac Surg. 2015;47:146–52.

    Article  Google Scholar 

  13. Fanous N, Husain SA, Ruzmetov M, Rodefeld MD, Turrentine MW, Brown JW. Anterior pericardial tracheoplasty for long-segment tracheal stenosis: long-term outcomes. J Thorac Cardiovasc Surg. 2010;139:18–23.

    Article  Google Scholar 

  14. Hayashida K, Kanda K, Yaku H, Ando J, Nakayama Y. Development of an in vivo tissue-engineered, autologous heart valve (the biovalve): preparation of a prototype model. J Thorac Cardiovasc Surg. 2007;134:152–9.

    Article  Google Scholar 

  15. Takiyama N, Mizuno T, Iwai R, Uechi M, Nakayama Y. In-body tissue-engineered collagenous connective tissue membranes (BIOSHEETs) for potential corneal stromal substitution. J Tissue Eng Regen Med. 2016;10:E518–26.

    Article  CAS  Google Scholar 

  16. Yamanami M, Yahata Y, Uechi M, Fujiwara M, Ishibashi-Ueda H, Kanda K, et al. Development of a completely autologous valved conduit with the sinus of Valsalva using in-body tissue architecture technology: a pilot study in pulmonary valve replacement in a beagle model. Circulation. 2010;122:S100–6.

    Article  Google Scholar 

  17. Nakayama Y, Oshima N, Tatsumi E, Ichii O, Nishimura T. iBTA-induced bovine Biosheet for repair of abdominal wall defects in a beagle model: proof of concept. Hernia. 2018; https://doi.org/10.1007/s10029-018-1799-8

Download references

Author information

Authors and Affiliations

  1. Department of Pediatric Surgery, Osaka University Graduate School of Medicine, 2-2 Yamadaoka Suita, Osaka, 565-0871, Japan

    Satoshi Umeda, Shohei Hiwatashi, Kengo Nakahata, Yuichi Takama & Hiroomi Okuyama

  2. Biotube Co., Ltd., 2-13-11, Shinkawa, Chuo, Tokyo, 104-0033, Japan

    Yasuhide Nakayama

  3. Division of Cell Engineering, Graduate School of Chemical Science and Engineering, Hokkaido University, Kita 13 Nishi 8, Kita-ku, Sapporo, 060-8628, Hokkaido, Japan

    Takeshi Terazawa

  4. Research Institute of Technology, Okayama University of Science, 1-1 Ridaicho, Kita-ku, Okayama, 700-0005, Japan

    Ryosuke Iwai

Authors
  1. Satoshi Umeda
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yasuhide Nakayama
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Takeshi Terazawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ryosuke Iwai
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Shohei Hiwatashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Kengo Nakahata
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yuichi Takama
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Hiroomi Okuyama
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hiroomi Okuyama.

Ethics declarations

Conflict of interest

All authors declare no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Umeda, S., Nakayama, Y., Terazawa, T. et al. Long-term outcomes of patch tracheoplasty using collagenous tissue membranes (biosheets) produced by in-body tissue architecture in a beagle model. Surg Today 49, 958–964 (2019). https://doi.org/10.1007/s00595-019-01818-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00595-019-01818-5

Keywords

Search

Navigation