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The diagnostic accuracy of oxidative stress biomarkers in sheep with pneumonic pasteurellosis

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Abstract

The goal of this study was to decide the diagnostic accuracy of oxidative stress biomarkers in infected sheep with Pasteurella multocida. Blood samples were collected from 56 sheep (36 naturally infected with Pasteurella multocida and 20 healthy controls) belonging to one farm in Al-Ahsa region, Saudi Arabia. Serum samples were evaluated for oxidative stress markers (malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) and catalase (CAT)). Additionally, nasal swabs and bronchoalveolar lavages were gathered from all sheep for bacteriological investigations. Receiver operating characteristic curve was used to assess the diagnostic performance of each parameter. The results showed significantly higher MDA level and lower SOD, GSH and CAT levels in pneumonic sheep compared to healthy controls. Both MDA and CAT showed the highest positive (r = 0.72) and negative (r = −0.71) correlation with disease status, respectively. Finally, MDA showed a relatively better test characteristic than other parameters, with an area under the curve (AUC = 0.93) indicating high degree of discrimination between control and pneumonic sheep. Conclusively, this study shed the light on the value of oxidative stress biomarkers in the diagnosis of naturally occurring pneumonic pasteurellosis in sheep.

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Acknowledgements

The authors would like also to thank the Deanship of Scientific Research, King Faisal University for the financial support of the research (Project No: 130031).

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Authors and Affiliations

  1. Department of Clinical Studies, College of Veterinary Medicine and Animal Resources, King Faisal University, P.O. Box: 400, 31982, Al-Ahsa, Saudi Arabia

    Wael M. El-Deeb & Ahmed Elmoslemany

  2. Department of Internal Medicine, Infectious Diseases and Fish Diseases, Faculty of Veterinary Medicine, Mansoura University, EL-Gomhorya St, Mansoura, Dakahlia, 35516, Egypt

    Wael M. El-Deeb

  3. Hygiene and Preventive Medicine Department, Faculty of Veterinary Medicine, Kafrelsheikh University, 35516, Kafr El Sheikh, Egypt

    Ahmed Elmoslemany

Authors
  1. Wael M. El-Deeb
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  2. Ahmed Elmoslemany
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Correspondence to Wael M. El-Deeb.

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All principles of ethical and professional conduct have been followed. This study is approved and funded by the Deanship of Scientific Research, King Faisal University (grant number 130031).

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The authors declare that they have no conflicts of interest.

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El-Deeb, W.M., Elmoslemany, A. The diagnostic accuracy of oxidative stress biomarkers in sheep with pneumonic pasteurellosis. Comp Clin Pathol 26, 363–367 (2017). https://doi.org/10.1007/s00580-016-2382-2

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  • DOI: https://doi.org/10.1007/s00580-016-2382-2

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