Abstract
This study was designed to evaluate the role of Peganum harmala extract on experimental skin wound healing. The investigation was carried out on 30 male Sprague-Dawley rats in the same conditions. Two uniform 7-mm diameter skin defect were created on the back of each animal by skin punch (total of 60 wounds). The extract was then applied once daily on half of the wounds. The animals were sacrificed on day 10 for histopathologic and biomechanical examinations. P. harmala extract significantly increased the number of fibroblasts and capillary buds and also decreased the epithelial gap which showed the better healing in treatment group. On the other hand, the improvement of biomechanical indices in treatment group revealed a significant increase in tensile strength of the wounds. It can be concluded that P. harmala is an effective herbal remedy in wound healing
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Babcock DT, Brock AR, Fish GS, Wang Y, Perrin L, Krasnow MA, Galko MJ (2008) Circulating blood cells function as a surveillance system for damaged tissue in Drosophila larvae. Proc Natl Acad Sci USA
Berrougui H, Herrera-Gonzalez MD, Marhuenda E, Ettaib A, Hmamouchi M (2002) Relaxant activity of methanolic extract from seeds of Peganum harmala on isolated rat aorta. Therapie 57:236–241
Berrougui H, Martin-Cordero C, Khalil A, Hmamouchi M, Ettaib A, Marhuenda E, Herrera MD (2006) Vasorelaxant effects of harmine and harmaline extracted from Peganum harmala L. seeds in isolated rat aorta. Pharmacol Res 54:150–157. doi:10.1016/j.phrs.2006.04.001
Derakhshanfar A, Mirzaei M (2008) Effect of Peganum harmala (wild rue) extract on experimental ovine malignant theileriosis: pathological and parasitological findings. Onderstepoort J Vet Res 75:67–72
El-Saad El-Rifaie M (1980) Peganum harmala: its use in certain dermatoses. Int J Dermatol 19:221–222. doi:10.1111/j.1365-4362.1980.tb00305.x
Lamchouri F, Settaf A, Cherrah Y, Zemzami M, Lyoussi B, Zaid A, Atif N, Hassar M (1999) Antitumour principles from Peganum harmala seeds. Therapie 54:753–758
Lamchouri F, Settaf A, Cherrah Y, El Hamidi M, Tligui N, Lyoussi B, Hassar M (2002) Experimental toxicity of Peganum harmala seeds. Ann Pharm Fr 60:123–129
Manske RH, Holmes HL (1952) The Alkaloids—Chemistry and Physiology vol. II. Academic, New York
Mirzaie M, Nosratabadi SJ, Derakhshanfar A, Sharif I (2007) Antileishmanial activity of Peganum harmala extract on the in vitro growth of Leishmania major promastigotes in comparison to a trivalent antimony drug. VETERINARSKI ARHIV 77:365
Shahverdi AR, Monsef-Esfahani HR, Nickavar B, Bitarafan L, Khodaee S, Khoshakhlagh N (2005) Antimicrobial activity and main chemical composition of two smoke condensates from Peganum harmala seeds. Z Naturforsch 60(C):707–710
Shi CC, Chen SY, Wang GJ, Liao JF, Chen CF (2000) Vasorelaxant effect of harman. Eur J Pharmacol 390:319–325. doi:10.1016/S0014-2999(99)00928-0
Shi CC, Liao JF, Chen CF (2001) Comparative study on the vasorelaxant effects of three harmala alkaloids in vitro. Jpn J Pharmacol 85:299–305. doi:10.1254/jjp. 85.299
Acknowledgement
The authors appreciate Professor David Weaver for his kind cooperation in revising the final version of the manuscript.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Derakhshanfar, A., Oloumi, M.M. & Mirzaie, M. Study on the effect of Peganum harmala extract on experimental skin wound healing in rat: pathological and biomechanical findings. Comp Clin Pathol 19, 169–172 (2010). https://doi.org/10.1007/s00580-009-0848-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00580-009-0848-1