Abstract
To determine the presence of constitutive nitric oxide synthase (NOS-3) mRNA in the caput, corpus, cauda epididymis, and vas deferens of adult bull, semiquantitative reverse transcription-polymerase chain reaction was performed on total RNAs isolated from those tissues. Expression of NOS-3 gene was exhibited in the samples of vas deferens and three parts of epididymis (caput, corpus, and cauda). Of course, the NOS-3 gene was detected in the part of the corpus weakly and was significant in comparison to other parts. NOS-3 mRNA in the part of cauda was nonsignificantly more than in other parts of the epididymis. Expression of this gene was significantly elevated in the two parts of the epididymis (caput and cauda) when compared with the vas deferens samples. These data could be the evidence for a different contribution of NOS-produced NO in the functions of epididymal segments and vas deferens.
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Acknowledgments
We are grateful to Mr. Yazdani and Mr. Khosravi for their excellent help in performing this study. This study was supported by Islamic Azad University (Garmsar branch) Research Grant.
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Hassanpour, H., Nikbakht Brujeni, G.H. & Mohammad Zade, A. Different gene expression of the constitutive nitric oxide synthase (NOS-3) in bull epididymis and vas deferens. Comp Clin Pathol 18, 7–11 (2009). https://doi.org/10.1007/s00580-008-0756-9
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DOI: https://doi.org/10.1007/s00580-008-0756-9