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Ectomycorrhizae of Tuber huidongense and T. liyuanum with Castanea mollissima and Pinus armandii

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Abstract

Tuber huidongense and T. liyuanum are common commercial white truffles in China that belong to the Rufum and Puberulum groups of the genus Tuber, respectively. Their mycorrhizae were successfully synthesized with two native trees—Castanea mollissima and Pinus armandii—under greenhouse conditions. The identities of the mycorrhizae were confirmed through internal transcribed spacer (ITS) sequence analyses, and their morphological characteristics were described. All of the obtained mycorrhizae have an interlocking pseudoparenchymatous mantle, which is a typical feature of truffle mycorrhizae. The mycorrhizae of T. huidongense on the two trees have hyaline branched emanating hyphae, similar to the documented mycorrhizae of the Rufum group. The unramified, spiky, and hyaline cystidia on the mycorrhizae of T. liyuanum with both C. mollissima and P. armandii further confirmed that this characteristic is constant for the mycorrhizae of the Puberulum group. The successful mycorrhizal syntheses on the two nut-producing trees will be of economic importance in the cultivation of the two truffles.

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References

  • Agerer R (ed) (1987–2008) Colour atlas of ectomycorrhizae. Einhorn Verlag, Schwäbisch Gmünd, 1st–13th

  • Benucci GMN, Bonito G, Baciarelli Falini L, Bencivenga M (2012) Mycorrhization of Pecan trees (Carya illinoinensis) with commercial truffle species: Tuber aestivum Vittad. and Tuber borchii Vittad. Mycorrhiza 22:383–392

    Article  PubMed  Google Scholar 

  • Blaschke H (1988) Tuber puberulum. In: Agerer R (ed) Color atlas of ectomycorrhizae: pl. 22. Einhorn Verlag + Druck GmbH, Schwäbisch Gmünd

  • Bonito G, Gryganskyi AP, Trappe JM, Vilgalys R (2010) A global meta-analysis of Tuber ITS rDNA sequences: species diversity, host associations and long-distance dispersal. Mol Ecol 19(22):4994–5008

    Article  CAS  PubMed  Google Scholar 

  • Bonito G, Trappe JM, Donovan S, Vilgalys R (2011) The Asian black truffle Tuber indicum can form ectomycorrhizas with North American host plants and complete its life cycle in nonnative soils. Fungal Ecol 4(1):83–93

    Article  Google Scholar 

  • Chen BT (2003) Report on inoculation and infection of truffles and establishment of truffle plantation. Guizhou Sci Technol 31(4):10–14

    Google Scholar 

  • Comandini O, Pacioni G (1997) Mycorrhizae of Asian black truffles. Tuber himalayense and T. indicum. Mycotaxon 63:77–86

    Google Scholar 

  • Deng XJ, Chen J, Yu FQ, Liu PG (2009) Notes on Tuber huidongense (Tuberaceae, Ascomycota), an endemic species from China. Mycotaxon 109:189–199

    Article  Google Scholar 

  • Deng XJ, Liu PG, Liu CY, Wang Y (2013) A new white truffle species, Tuber panzhihuanense from China. Mycol Prog 12:557–561

    Article  Google Scholar 

  • Deng XJ, Yu FQ, Liu PG (2014) Contribution to confirmed and synthesized on mycorrhizae of Tuber indicum s.l. with two dominated and subalpine broadleaf trees in Southwestern China. Am J Plant Sci 5:3269–3279

    Article  Google Scholar 

  • Di Massimo G, Bencivenga M, Tedeschini E, García-Montero LG, Manjón JL (1998) Nuova specie di Tuber importata dall´oriente. Micol Ital 27:13–18

    Google Scholar 

  • Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure from small quantities of fresh leaf tissues. Phytochem Bull 19:11–15

    Google Scholar 

  • Fan L, Cao JZ (2012) Two new species of white truffle from China. Mycotaxon 121:297–304

    Article  Google Scholar 

  • Fan L, Cao JZ, Liu YY, Li Y (2011) Two new species of Tuber from China. Mycotaxon 116:349–354

    Article  Google Scholar 

  • Fan L, Cao JZ, Zheng ZH, Li Y (2012a) Tuber in China: T. microspermum and T. microspiculatum spp. nov. Mycotaxon 119:391–395

    Article  Google Scholar 

  • Fan L, Hou CL, Cao JZ (2012b) Tuber sinoalbidum and T. polyspermum—new species from China. Mycotaxon 118:403–410

    Article  Google Scholar 

  • Fan L, Cao JZ, Li Y (2012c) Tuber microsphaerosporum and Paradoxa sinensis spp. nov. Mycotaxon 120:471–475

    Article  Google Scholar 

  • Fan L, Cao JZ, Yu J (2013a) Tuber in China: T. sinopuberulum and T. vesicoperidium spp. nov. Mycotaxon 121:255–263

    Article  Google Scholar 

  • Fan L, Cao JZ, Li Y (2013b) A reassessment of excavated Tuber species from China based on morphology and ITS rDNA sequence data. Mycotaxon 124:155

    Article  Google Scholar 

  • Fan L, Feng S, Cao JZ (2014) The phylogenetic position of Tuber glabrum sp. nov. and T. sinomonosporum nom. nov., two Paradoxa-like truffle species from China. Mycol Prog 13:241–246

    Article  Google Scholar 

  • García-Montero LG, Díaz P, Di Massimo G, García-Abril A (2010) A review of research on Chinese Tuber species. Mycol Prog 9:315–335

    Article  Google Scholar 

  • Gardes M, Bruns TD (1993) ITS primers with enhanced specificity for basidiomycetes—application to the identification of mycorrhizae and rusts. Mol Ecol 2:113–118

    Article  CAS  PubMed  Google Scholar 

  • Geng LY, Wang XH, Yu FQ, Deng XJ, Tian XF, Shi XF, Xie XD, Liu PG, Shen YY (2009) Mycorrhizal synthesis of Tuber indicum with two indigenous hosts, Castanea mollissima and Pinus armandii. Mycorrhiza 19:461–467

    Article  PubMed  Google Scholar 

  • Giomaro G, Zambonelli A, Sisti D, Cecchini M, Evangelista V, Stocchi V (2000) Anatomical and morphological characterization of mycorrhizas of five strains of Tuber borchii Vittad. Mycorrhiza 10:107–114

    Article  Google Scholar 

  • Guerin-Laguette A, Cummings N, Hesom-Williams N, Butler R, Wang Y (2013) Mycorrhiza analyses in New Zealand truffières reveal frequent but variable persistence of Tuber melanosporum in co-existence with other truffle species. Mycorrhiza 23:87–98

    Article  PubMed  Google Scholar 

  • Halász K, Bratek Z, Szego D, Rudnoy S, Racz I, Lasztity D, Trappe JM (2005) Tests of species concepts of the small, white, European group of Tuber spp. based on morphology and rDNA ITS sequences with special reference to Tuber rapaeodorum. Mycol Prog 4:281–290

    Article  Google Scholar 

  • He XY, Li HM, Wang Y (2004) Tuber zhongdianense sp. nov. from China. Mycotaxon 90:213–216

    Google Scholar 

  • Hu BF, Zhu ZR, Yuan XM, Yi XY, Zhang XM, Wang L, Fang S, Yang S, Liao WB (2004) Mycorrhizal synthesis of Tuber indicum and its effects on promoting seedling growth and preventing diseases. Guizhou Sci Technol 32(2):19–24

    Google Scholar 

  • Hu HT, Wang Y, Hu BY (2005) Cultivation of Tuber formosanum on limed soil in Taiwan. NZ J Crop Hortic Sci 33:363–366

    Article  Google Scholar 

  • Hu BF, Yin XY, Zhu ZR, Yuan XM, Yang AM, Jin TX, Hu G, Liao WB, Yang P (2006) Study on the inoculation technology of Tuber sp. for the mycorrhizal seedlings. Guizhou for Sci Technol 34(2):15–18, 26

    CAS  Google Scholar 

  • Jeandroz S, Murat C, Wang YJ, Bonfante P, Tacon FL (2008) Molecular phylogeny and historical biogeography of the genus Tuber, the ‘true truffles’. J Biogeogr 35:815–829

    Article  Google Scholar 

  • Katoh K, Toh H (2008) Recent developments in the MAFFT multiple sequence alignment program. Brief Bioinform 9(4):286–298

    Article  CAS  PubMed  Google Scholar 

  • Kovács GM, Jakucs E (2006) Morphological and molecular comparison of white truffle ectomycorrhizae. Mycorrhiza 16:567–574

    Article  PubMed  Google Scholar 

  • Lancellotti E, Iotti M, Zambonelli A, Franceschini A (2014) Characterization of Tuber borchii and Arbutus unedo mycorrhizas. Mycorrhiza 24:481–486

    Article  PubMed  Google Scholar 

  • Larsson E, Sundberg H (2011) Lyophyllum shimeji, a species associated with lichen pine forest in northern Fennoscandia. Mycosciense 52:289–295

    Article  Google Scholar 

  • Lefevre CK, Hall IR (2001) The status of truffle cultivation: a global perspective. Acta Hort 556:513–520

    Article  Google Scholar 

  • Li SH, Zheng LY, Liu CY, Wang Y, Lin L, Zhao YC, Zhang XL, Yang M, Xiong HK, Qing Y, Wang L, Zhou DQ (2014) Two new truffles species, Tuber alboumbilicum and Tuber pseudobrumale from China. Mycol Prog 13:1157–1163

    Google Scholar 

  • Lin Q, Han D, Liu CY (2008) Natural resources of truffles and analysis on the industry development in western Panzhihua. Panzhihua SciTech Inf 33(1):36–41

    Google Scholar 

  • Liu CY, Yang M, Tang P, Li XL, Xiao YJ, Zhen LY, Wang Y (2014) Research on mycorrhizal seedling cultivation and microstructure of Tuber panzhihuanense. Edible Fungi China 33(4):14–16

    Google Scholar 

  • Mabru D, Dupre C, Douet JP, Leroy P, Ravel C, Ricard JM, Medina B, Castroviejo M, Chevalier G (2001) Rapid molecular typing method for the reliable detection of Asiatic black truffle (Tuber indicum) in commercialized products: fruiting bodies and mycorrhizal seedlings. Mycorrhiza 11:89–94

    Article  CAS  Google Scholar 

  • Rauscher T, Chevalier G (1996) Tuber rufum. In: Agerer (ed) Colour atlas of ectomycorrhizae, Plate 89. Einhorn Verlag, Schwäbisch Gmünd

  • Rauscher T, Agerer R, Chevalier G (1995) Ektomykorrhizen von Tuber melanosporum, Tuber mesentericum und Tuber rufum (Tuberales) an Corylus avellana. Nova Hedwigia 61:281–322

    Google Scholar 

  • Rey JF (2001) Creation et origine de la commercialisation de la truffe de Chine en France et dans le monde. Proceedings du Ve Congrès International Science et Culture de la Truffe, Aix-en-Provence, 4–6 Mars 1999. Edited by Corvoisier M, Olivier JM, Chevalier G. Federation Française des trufficulteurs, Paris, pp 517–518

  • Swofford DL (2002) PAUP*.—phylogenetic analysis using parsimony (*and other methods), version 4.0b10. Sunderland: Sinauer Associates

  • Wang Y, Hall IR (2001) Tuber sinense and other species from Southwest China. Proceedings du Ve Congrès International Science et Culture de la Truffe, Aix-en-Provence, 4–6 March 1999. Edited by Corvoisier M, Olivier JM, Chevalier G. Federation Française des trufficulteurs, Paris, pp 115–116

  • Wang Y, He XY (2002) Tuber huidongense sp. nov. from China. Mycotaxon 83:191–194

    Google Scholar 

  • Wang Y, Moreno G, Riousset LJ, Manjón JL, Riousset G, Fourré G, Di Massimo G, García-Montero LG, Díez J (1998) Tuber pseudoexcavatum sp. nov.—a new species from China commercialized in Spain, France and Italy with additional comments on Chinese truffles. Cryptogam Mycol 19(1–2):113–120

    Google Scholar 

  • Wang YJ, Tan ZM, Murat C, Jeandroz S, Le Tacon F (2007) Molecular taxonomy of Chinese truffles belonging to the Tuber rufum and Tuber puberulum groups. Fungal Divers 24:301–328.

  • Wang Y (2008) Truffle research and cultivation in China. Proceedings 3º Congresso Internazionale di Spoleto sul Tartufo, Spoleto, 25– 28 Novembre 2008. Edited by Università Degli Studi di Perugia. Comunità Montana dei Monti Martani e del Serano, Spoleto, p 6

  • Wang Y, Liu PG, Chen J, Hu HT (2008). China—a newly emerging truffle-producing nation. Actes du Colloque La Culture de la Truffe dans le Monde, Brive-La-Gallarde, 2 February 2007. Edited by Reynal B, Chevalier G. Georges Lachaise, Brive-LaGallarde, pp 35–44

  • Wang XH (2013) Truffle Cultivation in China. In: Zambonelli A, Bonito GM (eds) Edible Ectomycorrhizal Mushrooms, Soil Biology, Berlin, Heidelberg: Springer Verlag. 34:227–240

  • Wang XH, Benucci GMN, Xie XD, Bonito G, Leisola M, Liu PG, Shamekh S (2013) Morphological, mycorrhizal and molecular characterization of Finnish truffles belonging to the Tuber anniae species-complex. Fungal Ecol 6:269–280

    Article  Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic, New York, pp 315–322

    Google Scholar 

  • Xu AS (1999) Notes on the genus of Tuber from Tibet. Mycosystema 18:361–365

    Google Scholar 

  • Zambonelli A, Salomoni S, Pisi A (1993) Caratterizzazione anatomomorphologica delle micorrize di Tuber spp. Su Quercus pubescens Willd. Micol Ital 22:73–90

    Google Scholar 

  • Zambonelli A, Salomoni S, Pisi A (1995) Caratterizzazione anatomomorfologica delle micorrize di Tuber borchii, Tuber aestivum, Tuber mesentericum, Tuber brumale, Tuber melanosporum su Pinus pinea. Micol Ital 24:119–137

    Google Scholar 

  • Zambonelli A, Pisi AN, Tibiletti E, Penjor D (1996) Anatomical morphological characterization of Tuber indicum Cooke and Massee mycorrhizae on Pinus pinea L. and Quercus cerris L. Proceedings of First International Conference on Mycorrhizae, Berkeley, 4–8 August 1996. Edited by Allen M, Bledsoe C, Bruns T, Molina R, Read D, Smith J, Smith S, Unesta T. University of California, Berkeley, pp 131

  • Zambonelli A, Tibiletti E, Pisi A (1997) Caratterizzazione anatomo-morfologica delle micorrize di Tuber indicum Cooke & Massee su Pinus pinea L. and Quercus cerris L. Micol Ital 28(1):29–36

    Google Scholar 

  • Zambonelli A, Iotti M, Amicucci A, Pisi A (1999) Caratterizzazione anatomo-morfologica delle micorrize di Tuber maculatum Vittad. su Ostrya carpinifolia Scop. Micol Ital 28:29–35

    Google Scholar 

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Acknowledgments

The work was supported by the National Natural Science Foundation of China (Nos. 31370070 and 31460551) and the Joint Founds of the National Science Foundation of China and Yunnan Province Government (No. U1202262).

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Authors and Affiliations

  1. College of Resource and Environment, Yunnan Agricultural University, Kunming, 650100, People’s Republic of China

    Shan-Ping Wan, Li Tang & Yi Zheng

  2. Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, People’s Republic of China

    Shan-Ping Wan, Fu-Qiang Yu & Ran Wang

  3. Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, People’s Republic of China

    Pei-Gui Liu & Xiang-Hua Wang

  4. New Zealand Institute for Plant and Food Research Limited, Christchurch, 8140, New Zealand

    Yun Wang

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  1. Shan-Ping Wan
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  2. Fu-Qiang Yu
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  3. Li Tang
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  4. Ran Wang
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Correspondence to Xiang-Hua Wang or Yi Zheng.

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Wan, SP., Yu, FQ., Tang, L. et al. Ectomycorrhizae of Tuber huidongense and T. liyuanum with Castanea mollissima and Pinus armandii . Mycorrhiza 26, 249–256 (2016). https://doi.org/10.1007/s00572-015-0663-2

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