Abstract
Key message
We conducted molecular characterization of Nicaraguan Pinus tecunumanii populations using microsatellite markers. Populations possess considerable genetic variation but there are risks associated with inbreeding and population fragmentation.
Abstract
We carried out a molecular characterization of three natural populations of Pinus tecunumanii using nine microsatellite markers. All studied populations occur in Nicaragua, where the species has declined primarily due to human-influenced factors. The results showed that there is a high amount of genetic variation in populations (expected heterozygosities 0.775–0.841), populations do not show significant differentiation (mean F ST 0.0073), apparently due to frequent gene flow or a more continuous distribution and homogenous genetic composition in the past, and inbreeding is common in all populations (F IS 0.705–0.780). The Structure analysis revealed that there is no evident clustering pattern among P. tecunumanii individuals. Although all studied populations possess a considerable amount of genetic variation, risks associated with inbreeding and population fragmentation should be acknowledged and a conservation strategy developed to safeguard the genetic resources of P. tecunumanii.
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Acknowledgments
We would like to thank the authorities from the Nicaragua-Finland Agrobiotechnology Program (NIFAPRO), Finnish Ministry of Foreign Affairs, University of Helsinki and Embassy of Finland in Nicaragua for supporting this research project.
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The authors declare no conflict of interest.
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Communicated by F. Canovas.
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Tijerino, A., Korpelainen, H. Molecular characterization of Nicaraguan Pinus tecunumanii Schw. ex Eguiluz et Perry populations for in situ conservation. Trees 28, 1249–1253 (2014). https://doi.org/10.1007/s00468-014-1005-2
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DOI: https://doi.org/10.1007/s00468-014-1005-2