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Urinary aquaporin-2 in children with acute pyelonephritis

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Abstract

Children with acute pyelonephritis develop polyuria and have reduced maximum urinary concentration capacity. We studied whether these abnormalities are associated with altered urinary excretion of the water channel aquaporin-2 (AQP2) in the renal collecting duct. AQP2 is the main target for antidiuretic action of arginine vasopressin (AVP), and the urinary excretion of this protein is believed to be an index of AVP signaling activity in the kidney. Children with acute pyelonephritis, aged 5–14 years, were examined for urinary flow rate, creatinine clearance, unchallenged urine osmolality, and urinary ion excretion. Urinary excretion of AQP2 was measured by dot immunoblotting technique. Studies were performed in the acute phase of pyelonephritis, in the same children after treatment, and in control patients. At the onset of pyelonephritis, urinary flow rate and solute excretion were increased, but the urinary osmolality was unchanged. The urinary level and urinary excretion of AQP2 was increased in acute pyelonephritis and decreased after treatment. Excretion of aquaporin-3 was unchanged, suggesting that the increase in AQP2 urinary excretion was not due to a shedding of collecting duct cells. The results suggest that a mechanism proximal to the collecting duct may be responsible for the polyuria observed in children with acute pyelonephritis. Increased urinary AQP2 levels suggest that a compensatory activation of apical plasma membrane targeting of AQP2 may occur in pyelonephritis.

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Acknowledgements

The study was supported by grants from INTAS, the Russian Foundation for Basic Research, the Swedish Medical Research Council, the Swedish Heart-Lung Foundation, and the Märta and Gunnar V. Philipsons Foundation.

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Authors and Affiliations

  1. Laboratory of Renal Physiology, I.M. Sechenov Institute of Evolutionary Physiology and Biochemistry, St. Petersburg, Russia

    Elena A. Rodionova, Elena I. Shakhmatova, Natalia Prutskova & Yuri Natochin

  2. Department of Pediatrics, St. Petersburg State Pediatric Medical Academy, St. Petersburg, Russia

    Alla A. Kuznetsova

  3. Department of Cell Biology, Institute of Anatomy, University of Aarhus, Aarhus, Denmark

    Søren Nielsen

  4. Nordic Center of Excellence for Research in Water Imbalance Related Disorders (WIRED); Department of Woman and Child Health, Karolinska Institutet, Stockholm, Sweden

    Ulla Holtbäck & Marina Zelenina

  5. Laboratory of Physiological Genetics, Institute of Cytology and Genetics, Novosibirsk, Russia

    Marina Zelenina

  6. Q2:09 Astrid Lindgren Children’s Hospital, 171 76 , Stockholm, Sweden

    Marina Zelenina

Authors
  1. Elena A. Rodionova
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  2. Alla A. Kuznetsova
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  3. Elena I. Shakhmatova
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  4. Natalia Prutskova
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  5. Søren Nielsen
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  6. Ulla Holtbäck
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  7. Yuri Natochin
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  8. Marina Zelenina
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Correspondence to Marina Zelenina.

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Rodionova, E.A., Kuznetsova, A.A., Shakhmatova, E.I. et al. Urinary aquaporin-2 in children with acute pyelonephritis. Pediatr Nephrol 21, 361–367 (2006). https://doi.org/10.1007/s00467-005-2101-8

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  • DOI: https://doi.org/10.1007/s00467-005-2101-8

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