Low-calorie diets are effective for weight loss in patients undergoing benign upper gastrointestinal surgery: a systematic review and meta-analysis

Background Obesity may increase surgical complexity in patients undergoing abdominal surgery by limiting visualization and increasing the risk of peri-operative complications. A preoperative reduction in weight and liver volume may improve surgical outcomes. The aim of our study was to evaluate the efficacy of a low-calorie diet (LCD) versus a very low-calorie diet (VLCD) in reducing weight and liver volume prior to laparoscopic surgery. Methods A systematic search was conducted using the following inclusion criteria: obese patients undergoing preoperative weight loss using a VLCD or LCD, evaluation of liver volume reduction, and the use of an imaging modality before and after the diet. Results A total of 814 patients from 21 different studies were included in this systematic review and meta-analysis, with 544 female patients (66.8%) and a mean age range between 24 and 54 years old. There was a total mean weight loss of 6.42% and mean liver volume reduction of 16.7%. Meta-analysis demonstrated that a preoperative diet (LCD or VLCD) significantly reduced weight [SMD = − 0.68; 95% CI (− 0.93, − 0.42), I2 = 82%, p ≤ 0.01] and liver volume [SMD = − 2.03; 95% CI (− 4.00, − 0.06), I2 = 94%, p ≤ 0.01]. When assessed individually, a VLCD led to significant weight reduction [SMD = − 0.79; CI (− 1.24; − 0.34), p ≤ 0.01, I2 = 90%], as did an LCD [SMD = − 0.60; CI (− 0.90; − 0.29), p ≤ 0.01, I2 = 68%). Similarly, there was a significant reduction in liver volume following a VLCD [SMD = − 1.40; CI (− 2.77, − 0.03), p ≤ 0.01, I2 = 96%], and an LCD [SMD = − 2.66; CI (− 6.13, 0.81), p ≤ 0.01, I2 = 93%]. However, there was no significant difference between the two regimens. Conclusions Preoperative restrictive calorie diets are effective in reducing weight and liver volume prior to laparoscopic surgery. Whilst a VLCD was better than an LCD at reducing both weight and liver volume, the difference was not significant. Graphical abstract Supplementary Information The online version contains supplementary material available at 10.1007/s00464-024-11016-1.


Graphical abstract
Low-calorie diets are effective for weight loss in patients undergoing benign upper gastrointestinal surgery: a systematic review and meta-analysis Obesity is a major risk factor for many chronic diseases like cardiovascular disease, type 2 diabetes, and some cancers [1].In 2015, 8.4% of the burden of disease in Australia was attributed to being overweight and/or morbidly obese [1].Moreover, one in three Australians were found to be obese in 2017-2018 which is a 12% increase from 1995.Laparoscopic surgery in obese patients poses challenges in visualization of structures in the surgical field and threatens damage to surrounding structures.It is also associated with adverse peri-operative outcomes such as anastomotic leak, poor healing, and longer operating times [2,3].As well, fatty liver disease is highly prevalent in patients who are obese which can add to the surgical challenges mentioned above [4].
The aim of our study was to evaluate the efficacy of an LCD compared to a VLCD in reduction of weight and liver volume in patients prior to benign upper gastrointestinal surgery.Secondary aims included analyses of modality used for liver imaging, diet tolerance, and whether the use of a preoperative diet improved surgical outcomes.

Methods
This review and meta-analysis fulfilled the proposed suggestions of the Cochrane Handbook for Systematic Reviews and Interventions [29] and was written using the PRISMA 2020 systematic review and meta-analysis guidelines [30] (Supplementary Fig. 1).

Databases
A methodical search was conducted on five different databases (PubMed, OVIDSP [Medline, JBI, PsychInfo], CINAHL, Cochrane and Scopus) to recognize relevant primary studies.For the preliminary search, broad terms such as [very low-calorie diet], [low-calorie diet], [liver volume], [weight loss], and [pre-surgery] were used.Subsequently, the synonyms of the above terms were used to conduct the final search on 21 November, 2022 (Supplementary Table 1).Identified studies were entered into COVIDENCE for a second reviewer.In addition, the references of the collected articles were manually explored to identify other relevant studies.

Criteria for selection
Inclusion criteria were the following: (1) primary articles written in English with full text available, (2) studies that prescribed a very low-calorie diet or low-calorie diet to achieve preoperative weight loss and liver volume reduction in patients planned for benign upper gastrointestinal surgery, (3) studies that included an objective measure of weight reduction (weight loss or body mass index (BMI) loss) and liver volume reduction (whole liver, left liver lobe, or visceral fat of liver) with an imaging modality (magnetic resonance imaging [MRI], computed tomography [CT], or ultrasound [US]), and (4) studies published between 1995 and 2022 with a sample size of at least 10 patients.Studies identified as reviews, non-peer-reviewed articles, conference abstracts, letters to editors, animal studies, using alternative methods of weight loss prior to surgery, including patients who underwent non-benign surgeries (i.e.cancer patients) were excluded.Following the systematic review, any studies missing weight reduction (in kg) and/or liver volume reduction (mL) raw values were excluded from the meta-analysis.

Study quality assessment: individual study risk of bias and publication bias
Two independent reviewers applied the Cochrane risk of bias tool [31] for randomized controlled trials (RCTs) and an adapted version of the methodological quality checklist outlined by Downs and Black [32] for non-RCTs to evaluate individual study risk of bias.For the Cochrane risk of bias tool, studies were classified as low risk (≥ 4 lowrisk domains), moderate risk (≥ 3 unclear risk domains), and high risk [(≥ 2 high-risk domains) (adapted from a similar systematic review [6])] based on their risk of bias.Following the modified Downs and Black checklist, a score between 20 and 22 was considered excellent, between 15 and 19 was considered good, between 11 and 14 was considered fair, and less than 11 was considered a poor-quality study (adapted from similar systematic review [6]).Any discrepancies between the two reviewers were subsequently discussed with the senior author and resolved.Publication bias was assessed with funnel plot asymmetry [33].

Data extraction
Data extraction was completed by one author and subsequently reviewed by a second investigator.Extracted data were entered into a pre-determined global data entry table which contained the following headings: authors, location, publication year, type of study, sample size, breakdown of sample size by sex (if available), diet used, calories, duration of diet, baseline mean weight/BMI, change in mean weight/ BMI after diet (in kg or kg/m 2 ), baseline mean liver volume, change in mean liver volume (in mL), methods of liver imaging (liver, left liver lobe, visceral fat of liver), and modality used (MRI, CT, US).Moreover, any secondary outcomes included in the collected studies were also compiled, such as tolerance of diet and view of surgical field.

Data synthesis and statistical analysis
Primary outcomes for this study included weight reduction and liver volume reduction from baseline to the patients' scheduled benign upper gastrointestinal surgery.Secondary outcomes comprised the modality of liver imaging, surgical outcomes, and diet tolerance.Weight reduction and liver volume reduction were recorded in kg and mL, respectively.Studies that reported BMI change, baseline weight, and weight post diet were calculated by multiplying the baseline BMI and post-diet BMI with the height 2 (if available).If studies reported median and confidence intervals, the median was considered equivocal to the mean and the IQR was divided by 1.35 to calculate the standard deviation.
The meta-analysis was undertaken with Review Manager 5.4.1 (RevMan).Standardized mean differences (SMD) with a 95% confidence interval (CI) were calculated and the statistical heterogeneity between studies was assessed using I 2 statistics.Hedge's g statistic was used to calculate the combined effect of preoperative diet on weight loss and liver volume reduction to account for the varied sample sizes amongst studies.To capture the impact of study heterogeneity, a random-effects model was applied for all studies and a p value of < 0.05 was deemed significant.A meta-regression analysis was performed to explore the impact of various factors on overall estimates.In addition, an influence analysis with a leave-one-out strategy was used to assess the influence of each study on the final combined outcome.The trim and fill method was used to address publication bias.

Results
A total of 1679 studies were found using the search terms described in the Methods section.In addition, 12 studies were added from reference lists of relevant reviews.All studies were uploaded onto COVIDENCE (a web-based streamlined collaborative platform for systematic reviews) and screened by two independent reviewers.After 211 duplicates were removed, titles and abstracts of 1468 studies were screened for relevance; 1406 studies were excluded.Fulltext review of 68 studies was conducted to finally include 21 studies for the systematic review and 20 studies for the meta-analysis (Fig. 1).
Of the 8 RCTs, two studies had a low risk of internal bias [11,25] whilst the remainder had a moderate risk of bias [8,14,17,19,22,26].Three of the moderate-risk studies [14,17,22] included randomization bias, one study contained bias in 'deviation from intended' [26], and the remaining two studies [8,19] contained detection bias (Supplementary Fig. 2).All of the RCTs were open label which increased their risk of bias for 'allocation concealment' and 'random sequence generation' categories (Suppl Fig. 2).
Of the non-RCT studies, one study was deemed excellent quality [12], eleven studies were rated as 'good' quality [9,10,15,16,20,23,24,27,28], and one study was rated as a 'fair' quality study [21].Lewis et al. [21] demonstrated high risk of bias in 'reporting', 'internal validity', and 'selection bias' (Suppl Table 2).Overall, all non-RCT studies lacked blinding of participants and assessors except Fris et al. [18].Finally, twelve of the thirteen studies did not calculate sufficient power to detect a clinically important effect, introducing a high risk of bias in 'power' (Supplementary Table 2).
Asymmetry was evident in the meta-analysis funnel plots which denoted a publication bias in the spectrum of studies published in this area (Fig. 2).Studies that demonstrated lower effects (low standard mean difference) of preoperative diet on weight reduction were more likely to be published compared to studies that showed a higher effect.However, the meta-analysis funnel plot for preoperative diet effect on liver volume reduction showed a more symmetrical distribution of studies which demonstrated a comparatively lower publication bias.The trim and fill method failed to adjust for publication bias in our meta-analysis as the adjusted effect size remained similar to the original estimate.

Preoperative diet
A variety of preoperative diets with a multitude of constituents and supplements were used by the studies included in this systematic review (Table 2).Many of the studies (twelve out of twenty-one studies) used an LCD formulation prior to surgery [8, 10, 11, 13-16, 19, 20, 25-27].Another six studies used a very low-calorie diet [12, 17-19, 21, 22], and the final five studies used novel diet regimens, i.e.VLCKD [23], KMED [24], MPED [28], BCM [26], and low carb [9].One study compared the effects of VLCD with LCD [19] and another compared BCM to Optifast® [26].The median duration for the diets was 4 weeks (range: 10-84 days) (Table 2).Diets varied in their calories, consistency, and composition.Sixteen studies prescribed a liquid diet [8, 10-15, 17-23, 26, 27], five studies prescribed a normal consistency diet [9, 17, 24,25,28], and one study did not include any description on the consistency of their diet [16].Of the liquid consistency diets, ten diets were solely liquid, and eight diets were a combination of liquid and normal consistency, with one study comparing the effects of liquid and normal consistency diet on weight loss [17].

Reduction in weight and liver volume
All studies reported a reduction in weight after consumption of the preoperative diet prescribed (Table 3).Mean baseline weight was 121.5 kg ± 16 kg and mean post-diet weight was 114 kg ± 16 kg.The mean %weight loss post diet was 6.42%.The mean baseline liver volume was 1,644 mL ± 403 mL with a post-diet mean liver volume of 1,328 mL ± 305 mL.The resulting average %loss in liver volume equated to 16.7% (Table 4).
A multivariate meta-regression analysis was performed to explore the impact of diet composition, diet duration, and research design on the final results.No significant difference was found in heterogeneity between the meta-regression analysis and the standard random-effects meta-analysis model (I 2 = 81.6% for weight, I 2 = 99.7% for liver volume), suggesting that these factors did not significantly account for the differences in true effect sizes.Similarly, an influence analysis was done to evaluate the impact of each study on the  [23] 117.4 109.9 6.30 Edholm et al. [14] 121.3 113.9 6.10 Colles et al. [12] 139.8 125 10.60 Ekici and Ferhatoglu [16] 124.4 118.9 4.42 Brody et al. [10] 122.7 118.9 3.10 Collins et al. [13] a 56 kg/m 2 49 kg/m 2 12.30 Benjaminov et al. [9] 129.19 124.45 3.67 Schiavo et al. [24] 131.9 119.65 9.29 Fris [18] 125.275 120.07 4.10 Abidin et al. [25] 112.54 110.33 1.96 Faria et al. [17] 114.46 110.7 3.49 Faria et al. [17] 107 overall combined outcome.The SMDs for weight and liver volume reduction remained the same regardless of which study was omitted from the analysis.The combined effects (− 0.67 for weight reduction and − 2.03 for liver volume reduction) remained consistent when using the leave-oneout strategy.

Discussion
Whilst our meta-analysis showed that a VLCD was more effective in both weight and liver volume reduction prior to benign upper gastrointestinal surgery, an LCD also resulted in significant weight and liver volume reduction, and there was no significant difference between the two diet regimens.This is an important finding as LCDs are often better tolerated than VLCDs, increasing patient compliance.Amongst the five different diet regimens seen in our systematic review, a VLCKD demonstrated the greatest efficacy in reducing weight per week of the diet used [23].This is not surprising given VLCKDs portend less than 400 kcal per day [3].The highest weight loss overall was not associated with the longest duration of the preoperative diet, nor the lowest calories consumed [13].Collins et al. demonstrated excellent reduction in baseline weight with an LCD set at 800 kcal/day, just over the threshold of a VLCD.That said, patients stayed on this diet for 9 weeks, longer than many of the other studies set at 4 weeks or less.Although counterintuitive, the findings of this study are supported by previous primary studies [18,21] and reviews [2,5] where a minimum period of two weeks for a preoperative diet was sufficient to achieve significant weight loss in patients.
Other findings deserve mention.First, restricted calorie diets had excellent tolerance amongst patients with only 17 out of 814 patients declining to participate due to diet intolerance (2.1%) (Table 1, Figs. 3, and 4).This is supported by recent reviews investigating the effects of preoperative diets on weight loss prior to bariatric surgery [3,5].Second, a significant decrease in liver function across most studies supports the notion that restrictive calorie diets reverse liver steatosis [17,22,23,26,38].Even though preoperative weight loss may provide an intra-operative surgical benefit, only five of 21 studies discussed better access to the hiatus, reduced surgical time, and easier liver retraction [9,13,14,17,21].In terms of liver volume reduction, the greatest reductions (43% and 47%) were seen in studies that used a liquid LCD for 4 weeks [10] and an MPED for 8 weeks [28], respectively.This again was neither the longest duration nor the lowest number of calories consumed as the study used a nutritional supplement (Nuvista) rather than a meal replacement.These studies demonstrated liver volume loss of more than double that seen in other studies.It is possible that the authors' measurement of liver volume with ultrasound was inaccurate as they used left liver lobe measurement only.This may not represent whole liver volume.A similar study showed only a 20% liver volume reduction [24].
Two studies reported liver volume reduction occurred during the first two weeks [15] and up to 4 weeks [20].Surprisingly, the second study demonstrated increased liver volumes from weeks 4 to 6 even though the diet regimen did not change [20]; this could have been due to diet noncompliance.One study reported that 90% of their patients achieved maximal liver volume loss within 3 weeks of an LCD [27].
Many different imaging techniques were used in our systematic review ranging from MRI, CT, and ultrasound.Although no obvious trends emerged, MRI seemed the most accurate for predicting overall liver volume [8,12,14,15,21,[25][26][27].This finding was supported by a recent review completed by van Wissen et al. [2].CT is also very good at gauging liver volume; however, it has the drawbacks of expense and radiation exposure [35].Finally, ultrasound has been shown to accurately measure degree of liver steatosis rather than volume [2,35].Ultrasound measurements of various segments of liver are operator dependent, and most studies do not use a validated method of calculating liver volume [10, 11, 16-18, 22-24, 28].A recent study by Childs et al. has shown that ultrasound is just as accurate in the measurement of liver volume compared to MRI [27], using a validated technique and equation [36,37].
There was great heterogeneity (weight reduction I 2 = 82%, liver volume reduction I 2 = 94%) in the meta-analysis amongst the studies which can be attributed to differences in baseline weight, number of calories consumed, duration of diet, consistency of diet, and whether the preoperative diet was administered as a supplement or replacement.Moreover, the imaging methods used also differed between studies.
Limitations of this systematic review include the heterogeneity of weight loss diets, unconventional presentation of data, lack of raw values, and lack of operator findings.For these reasons, the risk of interpretation bias was increased.Notable strengths of this study are use of PRISMA guidelines to conduct the review, use of PICO [patient, intervention, comparison, and outcome] formula to outline primary aims, use of two independent reviewers for data screening/collection/study appraisals, and the creation of standardized data collection tables to reduce individual reviewer bias.

Conclusion
Our systematic review and meta-analysis showed that restrictive calorie diets, either VLCD or LCD, are effective in weight and liver volume reduction prior to benign upper gastrointestinal surgery.Whilst VLCDs are more effective in weight and liver volume reduction, there was no significant difference when compared to LCDs.Patient compliance and satisfaction may be improved with an LCD compared to a VLCD but, overall, we found excellent tolerance amongst patients.
A= liver size pre diet B= liver size post diet VLCD= <800kcal/d; LCD= 800-1500kcal/d Whilst a very low-calorie diet (VLCD) was more effective than a low-calorie diet (LCD) in both weight and liver volume reduction, an LCD also resulted in significant weight (Study B, above) and liver volume reduction.There was no significant difference between the two diet regimens.Chowdhury N, Hasnan S, Ullah S, Thompson SK.Surgical Endoscopy, 2024 Keywords Low-calorie diet • Very low-calorie diet • Weight loss • Liver volume • Bariatric surgery , of which 3 trials researched the effects of two different diets on weight and liver volume reduction.Contreras et al. compared VLCD with LCD [19], Faria et al. compared normal consistency VLCD with a liquid VLCD [17], and Lange et al. compared low-energy diet (BCM) to Optifast®

Fig. 1
Fig. 1 PRISMA flow diagram showing the study selection process

Fig. 2
Fig. 2 Funnel plots of meta-analysis for weight and liver volume reduction of included studies denoting publication bias

Fig. 3
Fig. 3 Forest plots of meta-analysis of weight (Study A) and liver volume (Study B) reduction from baseline to post-diet follow-up.A preoperative diet significantly reduced weight (Study A) and liver volume (Study B)

Fig. 4
Fig. 4 Forest plots of meta-analysis comparing weight reduction from baseline to post very low-calorie diet (VLCD) (Study A) and low-calorie diet (LCD) (Study B), and liver volume reduction from baseline to post VLCD (Study C) and LCD (Study D).Both VLCDs

Table 1
Overview of included studies and patient demographics

Table 2
Composition of preoperative diets

Table 3
Post-diet weight loss

Table 5
Imaging modalities usedUS ultrasound, CT computed tomography scan, MRI magnetic resonance imaging