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Protection of trocar sites from gallbladder cancer implantation by sodium hyaluronate shill carboxymethylcellulose–based bioresorbable membrane (Seprafilm) in a nurine model

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Abstract

Background: The risk of port site metastasis in laparoscopic surgery for cancer patients is a problem that has yet to be resolved. We examined the protective effect of a sodium hyaluronate–based bioresorbable membrane (Seprafilm) on tumor cell implantation at laparoscopic trocar sites. Methods: Four 2-mm trocar sites were created in nude mice, and the peritoneal wounds were covered with different-sized pieces of Seprafilm. The protective effect of Seprafilm on the implantation of GB-d1 (a human gallbladder cancer cell line) at the trocar sites was assessed after 7 days. In addition, the effects of sodium hyaluronate and Seprafilm on the growth and motility of GB-d1 were examined in vitro. Results: Seprafilm significantly decreased the incidence of implantation compared with the control group. Histologically, Seprafilm was observed on days 1 and 3, as a sheet of gel that covered the injured peritoneum and muscle layer. In an invasion assay using Seprafilm, no cells were found to infiltrate through the gel sheet. Conclusion: Seprafilm protects peritoneal wounds by physically covering the injured peritoneum. Therefore, if Seprafilm were attached to the injured peritoneum after laparoscopic surgery for cancer patients, it might reduce port site metastasis.

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References

  1. Y Aoki H Shimura H Li K Mizumoto K Date M Tanaka (1999) ArticleTitleA model of port-site metastases of gallbladder cancer: the influence of peritoneal injury and its repair on abdominal wall metastases. Surgery 125 553–559 Occurrence Handle10.1067/msy.1999.98046 Occurrence Handle1:STN:280:DyaK1M3mtFegsg%3D%3D Occurrence Handle10330945

    Article  CAS  PubMed  Google Scholar 

  2. A Breborowicz H Rodela J Karon L Martis DG Oreopoulos (1997) ArticleTitleIn vitro simulation of the effect of peritoneal dialysis solution on mesothelial cells. Am J Kidney Dis 29 404–409 Occurrence Handle1:STN:280:ByiC1MzmtlY%3D Occurrence Handle9041217

    CAS  PubMed  Google Scholar 

  3. JW Burns MJ Colt LS Burgress KC Skinner (1997) ArticleTitlePreclinical evaluation of Seprafilm™ bioresorbable membrane. Eur J Surg 577 IssueIDSuppl 40–48

    Google Scholar 

  4. EB David (1997) ArticleTitleThe role of Seprafilm™ bioresorbable membrane in adhesion prevention. Eur J Surg 577 IssueIDSuppl 49–55

    Google Scholar 

  5. GS diZerega (1994) ArticleTitleContemporary adhesion prevention. Fertil Steril 61 219–235

    Google Scholar 

  6. T Fujisaki Y Tanaka K Fujii S Mine K Saito S Yamada U Yamashita T Irimura S Eto (1999) ArticleTitleCD44 stimulation induces integrin-mediated adhesion of colon cancer cell lines to endothelial cells by up-regulation of integrins and c-Met and activation of integrins. Cancer Res 59 4427–4434 Occurrence Handle1:CAS:528:DyaK1MXlvVSmsbw%3D Occurrence Handle10485493

    CAS  PubMed  Google Scholar 

  7. PJ Hewett ML Texler D Anderson G King BE Chatterton (1999) ArticleTitleIn vivo real-time analysis of intraperitoneal radiolabeled tumor cell movement during laparoscopy. Dis Colon Rectum 42 868–875 Occurrence Handle1:STN:280:DyaK1Mzjslaguw%3D%3D Occurrence Handle10411432

    CAS  PubMed  Google Scholar 

  8. G Hillerdal U Lindqvist A Engstrom-Laurent (1991) ArticleTitleHyaluronan in pleural effusions and in serum. Cancer 67 2410–2414 Occurrence Handle1:STN:280:By6C1M7kslU%3D Occurrence Handle2013045

    CAS  PubMed  Google Scholar 

  9. SC Hubbard JW Burns (2002) ArticleTitleEffects of a hyaluronan-based membrane (Seprafilm) on intraperitoneally disseminated human colon cancer cell growth in a nude mouse model. Dis Colon Rectum 45 334–344 Occurrence Handle12068190

    PubMed  Google Scholar 

  10. InstitutionalAuthorNameJapan Society for Endoscopic Surgery (1998) ArticleTitleThe questionnaire about endoscopic surgery: the 4th total result report. J Jpn Soc Endosc Surg 3 510–583

    Google Scholar 

  11. H Li H Shimura Y Aoki K Date K Matsumoto T Nakamura M Tanaka (1998) ArticleTitleHepatocyte growth factor stimulates the invasion of gallbladder carcinoma cells in vitro. Clin Exp Metastasis 16 74–82 Occurrence Handle1:CAS:528:DyaK1cXhvVyitLs%3D Occurrence Handle9502079

    CAS  PubMed  Google Scholar 

  12. RL Marquet MP van den Tol J Jeekell (2001) ArticleTitleSodium hyaluronate enhances colorectal tumour cell metastatic potential in vitro and in vivo. Br J Surg 88 246–250 Occurrence Handle10.1046/j.1365-2168.2001.01664.x Occurrence Handle1:STN:280:DC%2BD3M7kt1WisA%3D%3D Occurrence Handle11167875

    Article  CAS  PubMed  Google Scholar 

  13. SJ Neuhaus DI Watson T Ellis AM Rofe GG Jamieson (2000) ArticleTitleThe effect of immune enhancement and suppression on the development of laparoscopic port site metastases. Surg Endosc 14 439–443 Occurrence Handle10.1007/s004640000157 Occurrence Handle1:STN:280:DC%2BD3cvitlWhsA%3D%3D Occurrence Handle10858467

    Article  CAS  PubMed  Google Scholar 

  14. I Oikonomakis SD Wexner P Gervaz SY You M Secic P Giamundo (2002) ArticleTitleSeprafilm: a retrospective preliminary evaluation of the impact on short-term oncologic outcome in colorectal cancer. Dis Colon Rectum 45 1376–1380 Occurrence Handle12394438

    PubMed  Google Scholar 

  15. O Oksala T Salo R Tammi L Hakkinen M Jalkanen P Inki H Larjava (1995) ArticleTitleExpression of proteoglycans and hyaluronan during wound healing. J Histochem Cytochem 43 125–135 Occurrence Handle1:CAS:528:DyaK2MXjtlekurs%3D Occurrence Handle7529785

    CAS  PubMed  Google Scholar 

  16. H Shimura K Date K Matsumoto T Nakamura M Tanaka (1995) ArticleTitleInduction of invasive growth in a gallbladder cancer cell line by hepatocyte growth factor in vitro. Jpn J Cancer Res 86 662–669 Occurrence Handle1:CAS:528:DyaK2MXnsV2kt7k%3D Occurrence Handle7559084

    CAS  PubMed  Google Scholar 

  17. Y Shirai K Yoshida K Tsukada T Muto (1992) ArticleTitleInapparent carcinoma of the gallbladder: an appraisal of a radical second operation after simple cholecystectomy. Ann Surg 215 326–331 Occurrence Handle1:STN:280:By2B3MvpvVA%3D Occurrence Handle1558412

    CAS  PubMed  Google Scholar 

  18. LN Tseng FJ Berends P Wittich ND Bouvy RL Marquet G Kazemier HJ Bonjer (1998) ArticleTitlePort-site metastases: impact of local tissue trauma and gas leakage. Surg Endosc 12 1377–1380

    Google Scholar 

  19. RA Underwood JS Wu MP Wright MB Ruiz SM Pfister JM Connett JW Fleshman (1999) ArticleTitleSodium hyaluronate carboxymethylcellulose–based bioresorbable membrane (Seprafilm)—does it affect tumor implantation at abdominal wound sites? Dis Colon Rectum 42 614–618 Occurrence Handle1:STN:280:DyaK1M3nsVCrsg%3D%3D Occurrence Handle10344683

    CAS  PubMed  Google Scholar 

  20. P Ziprin PF Ridgway D Peck A Darzi (2001) ArticleTitleHyaluronan inhibits the adherence of a colon adenocarcinoma cell line to the mesothelium in vitro. Br J Surg 88 (Suppl.) 56

    Google Scholar 

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Authors and Affiliations

  1. First Department of Surgery, Fukuoka University School of Medicine, Nanakuma 7-45-1, Jonan-ku, Fukuoka, 814-0180, Japan

    T. Sasaki, H. Shimura, T. Tanaka, K. Nakashima, K. Matsuo & S. Ikeda

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  1. T. Sasaki
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  2. H. Shimura
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  3. T. Tanaka
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  4. K. Nakashima
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  5. K. Matsuo
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  6. S. Ikeda
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Correspondence to T. Sasaki.

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Sasaki, T., Shimura, H., Tanaka, T. et al. Protection of trocar sites from gallbladder cancer implantation by sodium hyaluronate shill carboxymethylcellulose–based bioresorbable membrane (Seprafilm) in a nurine model . Surg Endosc 18, 246–251 (2004). https://doi.org/10.1007/s00464-003-8128-7

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  • DOI: https://doi.org/10.1007/s00464-003-8128-7

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