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Carbon nanoparticles-based hydrogel nanocomposite induces bone repair in vivo

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Abstract

The main objective of the current study is to fabricate a 3D scaffold using alginate hydrogel implemented with carbon nanoparticles (CNPs) as the filler. The SEM imaging revealed that the scaffold possesses a porous internal structure with interconnected pores. The swelling value of the scaffolds (more than 400%) provides a wet niche for bone cell proliferation and migration. The in vitro evaluations showed that the scaffolds were hemocompatible (with hemolysis induction lower than 5%) and cytocompatible (inducing significant proliferative effect (cell viability of 121 ± 4%, p < 0.05) for AlG/CNPs 10%). The in vivo studies showed that the implantation of the fabricated 3D nanocomposite scaffolds induced a bone-forming effect and mediated bone formation into the induced bone defect. In conclusion, these results implied that the fabricated NFC-integrated 3D scaffold exhibited promising characteristics beneficial for bone regeneration and can be applied as the bone tissue engineering scaffold.

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Data availability

The datasets used or analyzed during the current study are available from the corresponding author on reasonable request.

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Author notes

  1. Zheng Zhou and Ao Zhou have contributed equally to this work.

Authors and Affiliations

  1. Department of Orthopaedic Surgery, Yangzhou Hongquan Hospital, Yangzhou, 225200, China

    Zheng Zhou

  2. Department of Bone and Soft Tissue Oncology, Cancer Hospital Affiliated to Chongqing University, Chongqing, 400020, China

    Ao Zhou

  3. Medical Laboratories Techniques Department, Al-Mustaqbal University College, Hilla, 51001, Babylon, Iraq

    Abduladheem Turki Jalil

  4. Department of Biophysics, College of Applied Sciences, University of Anbar, Ramadi, Iraq

    Marwan Mahmood Saleh

  5. Medical Laboratory Technology Department, College of Medical Technology, The Islamic University, Najaf, Iraq

    Marwan Mahmood Saleh

  6. Center for Joint Surgery, Southwest Hospital, Army Medical University, Chongqing, 400038, China

    Chengjun Huang

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  1. Zheng Zhou
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  2. Ao Zhou
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  3. Abduladheem Turki Jalil
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Zhou, Z., Zhou, A., Jalil, A.T. et al. Carbon nanoparticles-based hydrogel nanocomposite induces bone repair in vivo. Bioprocess Biosyst Eng 46, 577–588 (2023). https://doi.org/10.1007/s00449-022-02843-4

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