Abstract
Maltobionic acid (MBA) can be applied to various fields such as food, cosmetics, and pharmaceutical industries. In this study, whole-cell biocatalysis for MBA production was performed using recombinant Pseudomonas taetrolens homologously expressing quinoprotein glucose dehydrogenase (GDH). Various reaction parameters such as temperature, cell density, and cell harvest time, were optimized for improving MBA production. Under the optimized reaction conditions using pure maltose as a substrate, the MBA production titer, yield, and productivity of whole-cell biocatalyst (WCB) were 200 g/L, 95.6%, and 18.18 g/L/h, respectively, which were the highest compared to those reported previously. Productivity, a key factor for industrial MBA production, obtained from whole-cell biocatalysis in this study, was enhanced by approximately 1.9-fold compared to that obtained in our previous work (9.52 g/L/h) using the fermentation method. Additionally, the WCB could be reused up to six times without a significant reduction in MBA productivity, indicating that the WCB is very robust. Although MBA productivity (8.33 g/L/h) obtained from high-maltose corn syrup (HMCS) as a substrate was 45.8% of that using pure maltose, HMCS can be a better substrate for commercial MBA production because its price is only 1.1% of that of pure maltose. The results of this study using a WCB to convert maltose into MBA may support the development of a potential industrial process for more economically effective MBA production in the future.
Similar content being viewed by others
Abbreviations
- MBA:
-
Maltobionic acid
- GDH:
-
Quinoprotein glucose dehydrogenase
- WCB:
-
Whole-cell biocatalyst
References
Mehtio T, Toivari M, Wiebe MG, Harlin A, Penttila M, Koivula A (2016) Production and applications of carbohydrate-derived sugar acids as generic biobased chemicals. Crit Rev Biotechnol 36:904–916
Cañete-Rodríguez AM, Santos-Dueñas IM, Jiménez-Hornero JE, Ehrenreich A, Liebl W, García-García I (2016) Gluconic acid: properties, production methods and applications—an excellent opportunity for agro-industrial by-products and waste bio-valorization. Process Biochem 51:1891–1903
Sarenkova I, Ciprovica I (2018) The current status and future perspectives of lactobionic acid production : a review. Res Rural Dev 1:233–239
Cardoso T, Marques C, Dagostin JLA, Masson ML (2019) Lactobionic acid as a potential food ingredient: recent studies and applications. J Food Sci 84:1672–1681
Oh Y-R, Eom GT (2021) Identification of a lactose-oxidizing enzyme in Escherichia coli and improvement of lactobionic acid production by recombinant expression of a quinoprotein glucose dehydrogenase from Pseudomonas taetrolens. Enzyme Microb Technol 148:109828
Alonso S, Rendueles M, Díaz M (2013) Bio-production of lactobionic acid: current status, applications and future prospects. Biotechnol Adv 31:1275–1291
Oh Y-R, Jang Y-A, Hong SH, Eom GT (2020) Purification and characterization of a malate: quinone oxidoreductase from pseudomonas taetrolens capable of producing valuable lactobionic acid. J Agric Food Chem 68:13770–13778
Suehiro D, Okada M, Fukami K, Nakagawa T, Hayakawa T (2019) Maltobionic acid enhances intestinal absorption of calcium and magnesium in rats. Biosci Biotechnol Biochem 83:1766–1773
Fukami K, Kawai K, Takeuchi S, Harada Y, Hagura Y (2016) Effect of water content on the glass transition temperature of calcium maltobionate and its application to the characterization of non-arrhenius viscosity behavior. Food Biophys 11:410–416
Suehiro D, Kawase H, Uehara S, Kawase R, Fukami K, Nakagawa T, Shimada M, Hayakawa T (2020) Maltobionic acid accelerates recovery from iron deficiency-induced anemia in rats. Biosci Biotechnol Biochem 84:393–401
Green BA, Yu RJ, Van Scott EJ (2009) Clinical and cosmeceutical uses of hydroxyacids. Clin Dermatol 27:495–501
Suehiro D, Nishio A, Kawai J, Fukami K, Ohnishi M (2020) Effects of corn syrup solids containing maltobionic acid (maltobionic acid calcium salt) on bone resorption in healthy Japanese adult women: a randomized double-blind placebo-controlled crossover study. Food Sci Nutr 8:1030–1037
Fukami K, Suehiro D, Ohnishi M (2020) <i>In Vitro</i> utilization characteristics of maltobionic acid and its effects on bowel movements in healthy subjects. J Appl Glycosci 67:1–9
Tanabe K, Okuda A, Ken F, Yamanaka N, Nakamura S, Oku T (2020) Metabolic fate of newly developed nondigestible oligosaccharide, maltobionic acid, in rats and humans. Food Sci Nutr 8:3610–3616
Mirescu A, Prüße U (2007) A new environmental friendly method for the preparation of sugar acids via catalytic oxidation on gold catalysts. Appl Catal B 70:644–652
Zhang Z, Huber GW (2018) Catalytic oxidation of carbohydrates into organic acids and furan chemicals. Chem Soc Rev 47:1351–1390
Mao S, Liu Y, Hou Y, Ma X, Yang J, Han H, Wu J, Jia L, Qin H, Lu F (2018) Efficient production of sugar-derived aldonic acids by Pseudomonas fragi TCCC11892. RSC Adv 8:39897–39901
Kluyver AJ, de Ley J, Rijven A (1951) The formation and consumption of lactobionic and maltobionic acids byPseudomonas species. Antonie Van Leeuwenhoek 17:1–14
Oh YR, Jang YA, Lee SS, Kim JH, Hong SH, Han JJ, Eom GT (2020) Enhancement of lactobionic acid productivity by homologous expression of quinoprotein glucose dehydrogenase in Pseudomonas taetrolens. J Agric Food Chem 68:12336–12344
Oh Y-R, Jang Y-A, Hong SH, Eom GT (2020) High-level production of maltobionic acid from high-maltose corn syrup by genetically engineered Pseudomonas taetrolens. Biotechnology Reports 28:e00558
Keen NT, Tamaki S, Kobayashi D, Trollinger D (1988) Improved broad-host-range plasmids for DNA cloning in Gram-negative bacteria. Gene 70:191–197
Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428
Oh Y-R, Jang Y-A, Hong SH, Han JJ, Eom GT (2020) Efficient production of lactobionic acid using genetically engineered Pseudomonas taetrolens as a whole-cell biocatalyst. Enzyme Microb Technol 141:109668
Kim J-H, Jang Y-A, Seong S-B, Jang SA, Hong SH, Song JK, Eom GT (2020) High-level production and high-yield recovery of lactobionic acid by the control of pH and temperature in fermentation of Pseudomonas taetrolens. Bioprocess Biosyst Eng. https://doi.org/10.1007/s00449-020-02290-z
Jackson E, Ripoll M, Betancor L (2019) Efficient glycerol transformation by resting Gluconobacter cells. MicrobiologyOpen 8:e926
Wang J, Chae M, Sauvageau D, Bressler DC (2017) Improving ethanol productivity through self-cycling fermentation of yeast: a proof of concept. Biotechnol Biofuels 10:193
Abdel-Rahman MA, Hassan SE, Azab MS, Mahin AA, Gaber MA (2019) High improvement in lactic acid productivity by new alkaliphilic bacterium using repeated batch fermentation integrated with increased substrate concentration. Biomed Res Int 2019:7212870
Wang J, Chae M, Bressler DC, Sauvageau D (2020) Improved bioethanol productivity through gas flow rate-driven self-cycling fermentation. Biotechnol Biofuels 13:14
Carra S, Rodrigues DC, Beraldo NMC, Folle AB, Delagustin MG, de Souza BC, Reginatto C, Polidoro TA, da Silveira MM, Bassani VL, Malvessi E (2020) High lactobionic acid production by immobilized Zymomonas mobilis cells: a great step for large-scale process. Bioprocess Biosyst Eng. https://doi.org/10.1007/s00449-020-02323-7
Shaw J-F, Sheu J-R (1992) Production of high-maltose syrup and high-protein flour from rice by an enzymatic method. Biosci Biotechnol Biochem 56:1071–1073
Varela CA, Baez ME, Agosin E (2004) Osmotic stress response: quantification of cell maintenance and metabolic fluxes in a lysine-overproducing strain of Corynebacterium glutamicum. Appl Environ Microbiol 70:4222–4229
Wood JM (2015) Bacterial responses to osmotic challenges. J Gen Physiol 145:381–388
Lucht JM, Bremer E (1994) Adaptation of Escherichia coli to high osmolarity environments: osmoregulation of the high-affinity glycine betaine transport system ProU. FEMS Microbiol Rev 14:3–20
Funding
This work was supported in part by the R&D program of MOTIE/KEIT (10077291) and by the R&D program of KRICT (SS2142-10, BSF21-505) and by the R&D program of Ulsan-KRICT (US21-12, US21-12-01).
Author information
Authors and Affiliations
Contributions
YO: investigation, validation, data curation, writing—original draft. YJ: investigation, data curation, methodology. JKS: data curation, writing—original draft, and writing—review and editing. GTE: conceptualization, project administration, supervision, writing—original draft, writing—review and editing, funding acquisition, resources.
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Oh, YR., Jang, YA., Song, J.K. et al. Whole-cell biocatalysis using genetically modified Pseudomonas taetrolens for efficient production of maltobionic acid from pure maltose and high-maltose corn syrup. Bioprocess Biosyst Eng 45, 901–909 (2022). https://doi.org/10.1007/s00449-022-02708-w
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00449-022-02708-w