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Effects of turbulence fluctuation intensity in bioreactor of sewage treatment on physical and chemical properties of biofilms

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Abstract

The flow velocity plays an important role in the growth and characteristics of biofilm in the bioreactor as well as its treatment efficiency, which has been a hot research topic. In a sewage treatment bioreactor, the type of flow is usually turbulence. According to the methods of Reynolds decomposition, the flow velocity of turbulence consists of the time-mean velocity and fluctuation velocity, which usually exist simultaneously in real flow. However, the current research on the influence of turbulence flow velocity mainly focuses on the time-mean velocity, while the fluctuation velocity has not been reported because of very difficult to control. To this end, in this paper, a laboratory oscillating-grid turbulence (OGT) bioreactor with zero time-mean velocity and only fluctuation velocity was designed. In this bioreactor, the fluctuation velocity could be easily manipulated by varying the operational parameters of the grid. Based on the numerical simulation of Gas–liquid two-phase flow, the distributions of fluctuation velocity and corresponding turbulence fluctuation intensity, gas holdup, and Reynolds stress were obtained. After that, the effects of the turbulent fluctuation intensity on the biofilm thickness, density, and composition of extracellular polymeric substances (EPS) were studied experimentally. The results showed that turbulent fluctuation had a significant effect on the physical and chemical properties of biofilms, and the fluctuation velocity promoted the increase in the biofilm density and the content of protein and carbohydrates in EPS. This study was intended to provide theoretical support for the design and operation optimization of bioreactors.

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Authors and Affiliations

  1. Key Laboratory of Education Ministry of the Three Gorges Reservoir Region’s Eco-Environment, Chongqing University, Chongqing, 400045, China

    Chao Luo, Lisha Guo, Shi Zeng & Tianyu Long

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  1. Chao Luo
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  2. Lisha Guo
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  3. Shi Zeng
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  4. Tianyu Long
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Correspondence to Tianyu Long.

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Luo, C., Guo, L., Zeng, S. et al. Effects of turbulence fluctuation intensity in bioreactor of sewage treatment on physical and chemical properties of biofilms. Bioprocess Biosyst Eng 44, 1865–1874 (2021). https://doi.org/10.1007/s00449-021-02566-y

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