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Biodiversity, isolation and genome analysis of sulfamethazine-degrading bacteria using high-throughput analysis

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Abstract

Sulfamethazine (SM2) is one of the sulfonamide antibiotics that is frequently detected in aquatic environment. Given the complex structure of SM2 and its potential threat to the environment, it is necessary to determine the degradation behavior of high-concentration SM2. The mechanisms of community structure and diversity of activated sludge were analyzed. A novel SM2-degrading strain YL1 was isolated which can degrade SM2 with high concentration of 100 mg L−1. Strain YL1 was identified as Paenarthrobacter ureafaciens and there was also a significant increase in the genus during acclimation. Additional SM2 metabolic mechanisms and genomic information of YL1 were analyzed for further research. The succession of the community structure also investigated the effect of SM2 on the activated sludge. This result not only advances the current understanding of microbial ecology in activated sludge, but also has practical implications for the design and operation of the environmental bioprocesses for treatment of antimicrobial-bearing waste streams.

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Acknowledgements

The author would like to thank Prof. Yuanyuan Qu (Dalian University of Technology, Dalian, China) for her thoughtful review of this manuscript. This work was financially funded by the National Natural Science Foundation of China (No. 51478140).

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Authors and Affiliations

  1. State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin, 150090, China

    Lan Yu, Yingning Wang, Xiaoli Su, Yuanzhi Fu & Fang Ma

  2. College of Energy and Environmental Engineering, Hebei University of Engineering, Handan, 056038, China

    Haijuan Guo

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  1. Lan Yu
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Yu, L., Wang, Y., Su, X. et al. Biodiversity, isolation and genome analysis of sulfamethazine-degrading bacteria using high-throughput analysis. Bioprocess Biosyst Eng 43, 1521–1531 (2020). https://doi.org/10.1007/s00449-020-02345-1

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