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Influence of culture conditions towards optimal carotenoid production by Gordonia alkanivorans strain 1B

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Abstract

With the increasing awareness on the toxicity of several synthetic dyes, demand for pigments from natural sources, such as microbial carotenoids, has gained interest as a promising safe alternative colour additive. In this study, a surface response methodology based on the Doehlert distribution for two factors [% of glucose in a mixture of glucose + fructose (10 g/L total sugars), and sulfate concentration] was used towards the optimal carotenoids production by Gordonia alkanivorans strain 1B in the presence of light (400 lx). Time influence on pigment production by this bacterium was also evaluated, as well as the cell viability profile during longer incubation periods at optimal conditions. Indeed, the highest carotenoid production (2596–3100 μg/gDCW) was obtained when strain 1B was cultivated in the optimal conditions: glucose 10 g/L and sulfate ≥ 22 mg/L, in the presence of light for 19 days at 30 °C, 150 rpm. Flow cytometry showed that the highest production was somehow related with the cellular stress. These results highlight the great potential of strain 1B as a new hyperpigment producer to be exploited towards several applications.

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Acknowledgements

This work was financed by FEDER funds through POFC-COMPETE and by national funds through Fundação para a Ciência e a Tecnologia (FCT) in the scope of the project Carbon4Desulf—FCOMP-01-0124-FEDER-013932. Tiago P. Silva also acknowledges to FCT for his Ph.D. financial support (SFRH/BD/104977/2014).

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Authors and Affiliations

  1. LNEG–Instituto Nacional de Energia e Geologia, IP, Unidade de Bioenergia, Estrada do Paço do Lumiar, 22, 1649-038, Lisboa, Portugal

    Ana S. Fernandes, Susana M. Paixão, Tiago P. Silva, José C. Roseiro & Luís Alves

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  1. Ana S. Fernandes
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  2. Susana M. Paixão
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  3. Tiago P. Silva
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  4. José C. Roseiro
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  5. Luís Alves
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Correspondence to Susana M. Paixão or Luís Alves.

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Fernandes, A.S., Paixão, S.M., Silva, T.P. et al. Influence of culture conditions towards optimal carotenoid production by Gordonia alkanivorans strain 1B. Bioprocess Biosyst Eng 41, 143–155 (2018). https://doi.org/10.1007/s00449-017-1853-4

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  • DOI: https://doi.org/10.1007/s00449-017-1853-4

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