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Efficient biosynthesis of γ-decalactone in ionic liquids by immobilized whole cells of Yarrowia lipolytica G3-3.21 on attapulgite

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Abstract

In this study, the biosynthesis of γ-decalactone (GDL) was successfully conducted in an ionic liquid (IL)-containing cosolvent system using immobilized cells of Yarrowia lipolytica G3-3.21 on attapulgite (ATG). We found the immobilized Y. lipolytica G3-3.21 cells in N-butyl-pyridinium tetrafluoroborate ([BPy]BF4) solution gave the highest activity of C16-Acyl-CoA oxidase and the maximum yield of GDL. The optimum immobilization conditions for the highest yield of GDL were 20 g/L of ATG, 1.5 % of CaCl2 and 2 % of sodium alginate (NaAlg). The optimal [BPy]BF4 content, buffer pH, reaction temperature, shaking speed, castor oil and glucose contents were 7.5 %, 26 °C, 150 rpm, 100 g/L and 10 %, respectively. Under the optimized conditions, the GDL yield was up to 8.05 g/L. After ten times of reuse, the GDL yield was 7.51 g/L, corresponding to 93.3 % of that obtained in the first batch, suggesting a good reusability and potential for industrial applications.

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Acknowledgments

This work was financially supported by the Industrial Technology Program of Huaian, People’s Republic of China (HAG09040).

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Authors and Affiliations

  1. School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian, 223003, Jiangsu, People’s Republic of China

    Yuping Zhao & Changxing Jiang

  2. Laboratory of Brewing Microbiology and Applied Enzymology, School of Biotechnology, Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214036, Jiangsu, People’s Republic of China

    Yuping Zhao & Yan Xu

  3. Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaiyin Institute of Technology, Huaian, 223003, Jiangsu, People’s Republic of China

    Yuping Zhao & Changxing Jiang

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  1. Yuping Zhao
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  2. Yan Xu
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Correspondence to Yuping Zhao.

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Zhao, Y., Xu, Y. & Jiang, C. Efficient biosynthesis of γ-decalactone in ionic liquids by immobilized whole cells of Yarrowia lipolytica G3-3.21 on attapulgite. Bioprocess Biosyst Eng 38, 2045–2052 (2015). https://doi.org/10.1007/s00449-015-1431-6

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