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Population dynamics of methanogens and methane formation associated with different loading rates of organic acids along with ammonia: redundancy analysis

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Abstract

In anaerobic processes, the population dynamics of methanogens in the methanogenic stage were monitored along with hydraulic retention times (HRTs) shift. Decreasing HRTs increased the loading rates of volatile fatty acids (VFAs) and ammonia. Methanomicrobiales (MMB) began to be dominant at longer than 12.5 days HRT, Methanosarcinales (MSL) were dominant at 8, 10, and 12.5 days HRT, and Methanobacteriales (MBT) were dominant at shorter than 6 days HRT. Increased loading rates of VFAs and ammonia increased MBT, decreased MMB, and had no significant effect on MSL. Maximal daily methane production was observed at 1.57 L/L when MSL copy numbers also reached 3.60 × 107 copy/mL as a peak, which were expressed as positive correlation between DMA and MSL. No sooner had methane yield (MY) increased from 1.15 to 1.32 L/g VSremoved along with HRT reduction from 25 to 22.5 days, then MY gradually decreased from 1.32 to 0.04 L/g VSremoved.

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Fig. 1
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Abbreviations

AD:

Anaerobic digestion

CCA:

Canonical correspondence analysis

COD:

Chemical oxygen demand

CSTRs:

Continuously stirred tank reactors

DMP:

Daily methane production

HRT:

Hydraulic retention time

MBT:

Methanobacteriales

MCC:

Methanococcales

MMB:

Methanomicrobiales

MSL:

Methanosarcinales

MY:

Methane yield

RDA:

Redundancy analysis

TVFAs:

Total volatile fatty acids

VFAs:

Volatile fatty acids

VS:

Volatile solids

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Acknowledgments

This work was supported by the New & Renewable Energy Technology R&D program of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) grant funded by the Korean Ministry of Knowledge Economy (Grant No. 20103020090050) and also was supported by the Advanced Biomass R&D Center (ABC) of Global Frontier Project funded by the Ministry of Science, ICT and Future Planning (ABC-2010-0029728).

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Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering, KAIST, Daejeon, 305-701, Republic of Korea

    Woong Kim

  2. Infrastructure and Environment Division, School of Engineering, University of Glasgow, Glasgow, G12 8LT, UK

    Seung Gu Shin

  3. School of Environmental Science and Engineering, Pohang University of Science and Technology, Pohang, Gyeongbuk, 790-784, South Korea

    Kyungjin Cho, Gyuseong Han & Seokhwan Hwang

Authors
  1. Woong Kim
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  2. Seung Gu Shin
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  3. Kyungjin Cho
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  4. Gyuseong Han
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  5. Seokhwan Hwang
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Corresponding author

Correspondence to Seokhwan Hwang.

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W. Kim and S. G. Shin contributed equally to this work.

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Kim, W., Shin, S.G., Cho, K. et al. Population dynamics of methanogens and methane formation associated with different loading rates of organic acids along with ammonia: redundancy analysis. Bioprocess Biosyst Eng 37, 977–981 (2014). https://doi.org/10.1007/s00449-013-1061-9

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  • DOI: https://doi.org/10.1007/s00449-013-1061-9

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