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Molecular characterization of manganese peroxidases from white-rot fungus Polyporus brumalis

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Abstract

The cDNAs of six manganese-dependent peroxidases (MnPs) were isolated from white-rot fungus Polyporus brumalis. The MnP proteins shared similar properties with each other in terms of size (approximately 360–365 amino acids) and primary structure, showing 62–96 % amino acid sequence identity. RT-PCR analysis indicated that these six genes were predominantly expressed in shallow stationary culture (SSC) in a liquid medium. Gene expression was induced by treatment with dibutyl phthalate (DBP) and wood chips. Expression of pbmnp4 was strongly induced by both treatments, whereas that of pbmnp5 was induced only by DBP, while pbmnp6 was induced by wood chips only. Then, we overexpressed pbmnp4 in P. brumalis under the control of the GPD promoter. Overexpression of pbmnp4 effectively increased MnP activity; the transformant that had the highest MnP activity also demonstrated the most effective decolorization of Remazol Brilliant Blue R dye. Identification of MnP cDNAs can contribute to the efficient production of lignin-degradation enzymes and may lead to utilization of basidiomycetous fungi for degradation of lignin and numerous recalcitrant xenobiotics.

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Acknowledgments

This study was carried out with the grant funded by Korea Forest Research Institute.

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Authors and Affiliations

  1. Division of Wood Chemistry and Microbiology, Department of Forest Products, Korea Forest Research Institute, Seoul, 130-712, South Korea

    Sun-Hwa Ryu, Boyeong Kim & Myungkil Kim

  2. Department of Agricultural Biotechnology and Center for Food and Bioconvergence, Seoul National University, Seoul, 151-921, South Korea

    Sun-Hwa Ryu & Jin-Ho Seo

Authors
  1. Sun-Hwa Ryu
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  2. Boyeong Kim
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  4. Jin-Ho Seo
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Correspondence to Myungkil Kim or Jin-Ho Seo.

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Ryu, SH., Kim, B., Kim, M. et al. Molecular characterization of manganese peroxidases from white-rot fungus Polyporus brumalis . Bioprocess Biosyst Eng 37, 393–400 (2014). https://doi.org/10.1007/s00449-013-1004-5

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