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Improving arachidonic acid accumulation in Mortierella alpina through B-group vitamin addition

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Abstract

To improve the arachidonic acid (ARA) accumulation in Mortierella alpina, a mixed B-group vitamin addition strategy was developed. The ARA titer reached up to 10.0 g/L, 1.7-fold of the control. At the same time, the highest specific activities of key enzymes involved in ARA biosynthesis, including malic enzyme, glucose-6-phosphate dehydrogenase and ATP: citrate lyase, were 63.3, 38.6 and 53.7% higher than the control, respectively. The possible vitamin triggered improved ARA accumulation mechanism was thus elucidated that B-group vitamins could function as the cofactors of the key enzymes involved in ARA biosynthesis, or precursors for the formation of NADPH and acetyl-CoA which were crucial for ARA synthesis, and strengthened the related metabolic flux.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (Nos. 20936002; 21006049), the National Basic Research Program of China (No. 2011CBA00802), the National Key Technology Support Program of China (No. 2011BAD23B03), and the Priority Academic Program Development of Jiangsu Higher Education Institutions. H. Huang was supported by the Fok Ying Tung Education Foundation (No. 123014), and the Program for New Century Excellent Talents in University from the Ministry of Education of China (No. NCET–09–0157).

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Authors and Affiliations

  1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Nanjing, 210009, People’s Republic of China

    Yan Zeng, Xiao-Jun Ji, Shu-Mei Chang, Zhi-Kui Nie & He Huang

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  1. Yan Zeng
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  2. Xiao-Jun Ji
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  5. He Huang
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Correspondence to He Huang.

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Zeng, Y., Ji, XJ., Chang, SM. et al. Improving arachidonic acid accumulation in Mortierella alpina through B-group vitamin addition. Bioprocess Biosyst Eng 35, 683–688 (2012). https://doi.org/10.1007/s00449-011-0648-2

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