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Mycogenesis of gold nanoparticles using a phytopathogen Alternaria alternata

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Abstract

The development of an eco-friendly and reliable process for the synthesis of gold nanomaterials (AuNPs) using microorganisms is gaining importance in the field of nanotechnology. In the present study, AuNPs have been synthesized by bio-reduction of chloroauric acid (HAuCl4) using the fungal culture filtrate (FCF) of Alternaria alternata. The synthesis of the AuNPs was monitored by UV–visible spectroscopy. The particles thereby obtained were characterized by UV, dynamic light scattering (DLS), X-ray diffraction (XRD), energy dispersive X-ray (EDX) analysis, Fourier transform infrared (FTIR) spectroscopy, atomic force microscopy (AFM) and transmission electron microscopy (TEM). Energy-dispersive X-ray study revealed the presence of gold in the nanoparticles. Fourier transform infrared spectroscopy confirmed the presence of a protein shell outside the nanoparticles which in turn also support their stabilization. Treatment of the fungal culture filtrate with aqueous Au+ ions produced AuNPs with an average particle size of 12 ± 5 nm. This proposed mechanistic principal might serve as a set of design rule for the synthesis of nanostructures with desired architecture and can be amenable for the large scale commercial production and technical applications.

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Abbreviations

FCF:

Fungal culture filtrate

DLS:

Dynamic light scattering

XRD:

X-ray diffraction

EDX:

Energy dispersive X-ray

FTIR:

Fourier transform infrared

AFM:

Atomic force microscopy

TEM:

Transmission electron microscopy

HAuCl4 :

Chloroauric acid

AuNPs:

Gold nanoparticles

References

  1. Parak WJ, Gerion D, Pellegrino T, Zanchet D, Micheel C, Williams CS, Boudreau R, Le Gros MA, Larabell CA, Alivisatos PA (2003) Biological applications of colloidal nano crystals. Nanotechnol 14:15–27

    Article  Google Scholar 

  2. Gao X, Yang L, Petros JA, Marshall FF, Simons JW, Nie S (2005) In vivo molecular and cellular imaging with quantum dots. Curr Opin Biotechnol 16:63–72

    Article  CAS  Google Scholar 

  3. Alivisatos PA (2004) The use of nanocrystals in biological detection. Nat Biotechnol 2:47–52

    Article  Google Scholar 

  4. Salata OV (2004) Applications of nanoparticles in biology and medicine. J Nanobiotechnol 2:3

    Article  Google Scholar 

  5. Prescher JA, Bertozzi CR (2005) Chemistry in living systems. Nat Chem biol 1:13–21

    Article  CAS  Google Scholar 

  6. Thrall JH (2004) Nanotechnology and Medicine. Radiol 230:315–318

    Article  Google Scholar 

  7. El-Sayed IH, Huang X, El-Sayed MA (2005) Surface plasmon resonance scattering and absorption of anti-EGFR antibody conjugated AuNPs in cancer diagnostics: applications in oral cancer. Nano Lett 5:829–834

    Article  CAS  Google Scholar 

  8. Das RK, Gogoi N, Bora U (2011) Green synthesis of gold nanoparticles using Nyctanthes arbortristis flower extract. Bioprocess Biosyst Eng 34:615–619

    Article  CAS  Google Scholar 

  9. Sun Y, Xia Y (2002) Shape-controlled synthesis of gold and silver nanoparticles. Science 298:2176–2179

    Article  CAS  Google Scholar 

  10. Selvakannan PR, Mandal S, Pasricha R, Adyanthaya SD, Sastry M (2002) One-step synthesis of hydrophobized gold nanoparticles of controllable size by the reduction of aqueous chloroaurate ions by hexadecylaniline at the liquid–liquid interface. Chem Commun 13:1334–1335

    Article  Google Scholar 

  11. Korbekandi H, Iravani S, Abbasi S (2009) Production of nanoparticles using organisms. Crit Rev Biotech 29:279–306

    Article  CAS  Google Scholar 

  12. Basu N, Bhattacharya R, Mukherjee P (2008) Protein-mediated autoreduction of gold salts to gold nanoparticles. Biomed Mater 3:34105

    Article  Google Scholar 

  13. Kim J, Rheem Y, Yoo B, Chong Y, Bozhilov KN, Kim D, Sadowsky MJ, Hur HG, Myung NV (2010) Peptide-mediated shape- and size-tunable synthesis of gold nanostructures. Acta Biomater 7:2681–2989

    Article  Google Scholar 

  14. Verma VC, Kharwar RN, Gange AC (2010) Biosynthesis of antimicrobial silver nanoparticles by the endophytic fungus Aspergillus clavatus. Nanomed 5:33–40

    Article  CAS  Google Scholar 

  15. Huang P, Kong Y, Li Z, Gao F, Cui D (2010) Copper selenide nanosnakes: bovine serum albumin-assisted room temperature controllable synthesis and characterization. Nanoscale Res Lett 5:949–956

    Article  CAS  Google Scholar 

  16. Narayanan KB, Sakthivel N (2010) Biological synthesis of metal nanoparticles by microbes. Adv Colloid Interfac 156:1–13

    Article  CAS  Google Scholar 

  17. Chakraborty N, Banerjee A, Lahiri S, Panda A, Ghosh AN, Pal R (2009) Biorecovery of gold using cyanobacteria and an eukaryotic alga with special reference to nanogold formation–a novel phenomenon. J Appl Phycol 21:145–152

    Article  CAS  Google Scholar 

  18. Acharya K, Sarkar J, Deo SS (2009) Mycosynthesis of nanoparticles. In: Bhowmik PK, Basu SK, Goyal A (eds) Advances in biotechnology. Bentham Science, USA, pp 204–215

  19. Verma VC, Singh SK, Solanki R, Prakash S (2011) Biofabrication of anisotropic gold nanotriangles using extract of endophytic Aspergillus clavatus as a dual functional reductant and stabilizer. Nano Res Lett 6:16–23

    Article  Google Scholar 

  20. Sastry M, Ahmad A, Khan I, Kumar R (2003) Biosynthesis of metal nanoparticles using fungi and actinomycete. Curr Sci 85:162–170

    CAS  Google Scholar 

  21. Saha S, Sarkar J, Chattopadhyay D, Patra S, Chakraborty A, Acharya K (2010) Production of silver nanoparticles by a phytopathogenic fungus Bipolaris nodulosa and its antimicrobial activity. Dig J Nanomater Biostruct 5:887–895

    Google Scholar 

  22. Sarkar J, Chattopadhyay D, Patra S, Deo SS, Sinha S, Ghosh M, Mukherjee A, Acharya K (2011) Alternaria alternata mediated synthesis of protein capped silver nanoparticles and their genotoxic activity. Dig J Nanomat Biostruct 6:563–573

    Google Scholar 

  23. Sarkar J, Dey P, Saha S, Acharya K (2011) Mycosynthesis of selenium nanoparticles. Micro Nano Lett 6:599–602

    Article  CAS  Google Scholar 

  24. Corti CW, Holliday RJ, Thompson DT (2002) Developing new industrial applications for gold: gold nanotechnology. Gold Bull 35:111–117

    Article  CAS  Google Scholar 

  25. Corti CW, Holliday RJ (2004) Commercial aspects of gold applications: from materials science to chemical science. Gold Bull 37:20–26

    Article  CAS  Google Scholar 

  26. Cortie MB (2004) The weird world of nanoscale gold. Gold Bull 37:12–19

    Article  CAS  Google Scholar 

  27. Huang H, Yang X (2005) One-step shape control synthesis of gold nanoparticles stabilized by 3-thiopheneacetic acid. Coll Surf A: Physicochem Eng Asp 255:11–17

    Article  CAS  Google Scholar 

  28. Daniel M-C, Astruc D (2004) Gold nanoparticles: assembly, supramolecular chemistry, quantam-size related properties, and applications toward biology, catalysis, and nanotechnology. Chem Rev 104:293–346

    Article  CAS  Google Scholar 

  29. Mukherjee P, Ahmad A, Mandal D, Senapati S, Sainkar SR, Khan MI, Ramani R, Parischa R, Kumar PAV, Alam M, Sastry M, Kumar R (2001) Bioreduction of AuCl4 − Ions by the fungus, Verticillium sp. and surface trapping of the gold nanoparticles formed. Angew Chem Int Ed 40:3585–3588

    Article  CAS  Google Scholar 

  30. Mukherjee P, Senapati S, Mandal D, Ahmad A, Khan MI, Kumar R, Sastry M (2002) Extracellular synthesis of gold nanoparticles by the fungus Fusarium oxysporum. Chem Bio Chem 3:461–463

    CAS  Google Scholar 

  31. Bhambure R, Bule M, Shaligram N, Kamat M, Singhal R (2009) Extracellular biosynthesis of gold nanoparticles using Aspergillus niger–its characterization and stability. Chem Eng Technol 32:1036–1041

    Article  CAS  Google Scholar 

  32. Shankar SS, Ahmad A, Pasricha R, Sastry M (2003) Bioreduction of chloroaurate ions by geranium leaves and its endophytic fungus yields gold nanoparticles of different shapes. J Mater Chem 13:1822–1826

    Article  CAS  Google Scholar 

  33. Ahmad A, Satyajyoti S, Khan MI, Rajiv K, Sastry M (2005) Extra-/intracellular biosynthesis of gold nanoparticles by an alkalotolerant fungus, Trichothecium sp. J Biomed Nanotechnol 1:47–53

    Article  CAS  Google Scholar 

  34. Maiti CK, Sen S, Acharya R, Acharya K (2007) First report of Alternaria alternata causing leaf spot on Stevia rebaudiana. Plant Pathol 56:723

    Article  Google Scholar 

  35. Song JY, Jang H-K, Kim BS (2009) Biological synthesis of gold nanoparticles using Magnolia kobus and Diopyros kaki leaf extracts. Proc Biochem 44:1133–1138

    Article  CAS  Google Scholar 

  36. Sathyavathi R, Krishna MB, Rao SV, Saritha R, Rao DN (2010) Biosynthesis of silver nanoparticles using Coriandrum Sativum leaf extract and their application in nonlinear optics. Adv Sci Lett 3:01–06

    Article  Google Scholar 

  37. Renuga Devi TS, Gayathri S (2010) FTIR and FT-Raman spectral analysis of paclitaxel drugs. Int J Pharm Sci Rev Res 2:106–110

    CAS  Google Scholar 

  38. Pandey KK (1999) A study of chemical structure of soft and hardwood and wood polymers by ftir spectroscopy. J Appl Pol Sci 71:1969–1975

    Article  CAS  Google Scholar 

  39. Cai S, Singh BR (2004) A distinct utility of the amide III infrared band for secondary structure estimation of aqueous protein solutions using partial least squares methods. Biochem 43:2541–2549

    Article  CAS  Google Scholar 

  40. Singh AK, Talat M, Singh DP, Srivastava ON (2010) Biosynthesis of gold and silver nanoparticles by natural precursor clove and their functionalization with amine group. J Nanopart Res 12:1667–1675

    Article  CAS  Google Scholar 

  41. Sanghi R, Verma P (2009) Biomimetic synthesis and characterization of protein capped silver nanoparticles. Biores Technol 100:501–504

    Article  CAS  Google Scholar 

  42. Gole A, Dash C, Ramakrishnan V, Sainkar SR, Mandle AB, Rao M (2001) Pepsin-gold colloid conjugates: preparation, characterization, and enzymatic. Langmuir 17:1674–1679

    Article  CAS  Google Scholar 

  43. Ahmad A, Senapati S, Khan MI, Kumar R, Sastry M (2003) Extracellular biosynthesis of monodisperse gold nanoparticles by a novel extremophilic actinomycete, Thermomonospora sp. Langmuir 19:3550–3553

    Article  CAS  Google Scholar 

  44. Xie J, Lee JY, Wang DIC, Ting YP (2007) High-yield synthesis of complex gold nanostructures in a fungal system. J Phys Chem C 111:16858–16865

    Article  CAS  Google Scholar 

  45. Kumar SA, Abyaneh MK, Gosavi SW, Kulkarni SK, Ahmad A, Khan MI (2007) Sulfite reductase-mediated synthesis of gold nanoparticles capped with phytochelatin. Biotechnol Appl Biochem 47:191–195

    Article  CAS  Google Scholar 

  46. Kumar SA, Abyaneh MK, Gosavi SW, Kulkarni SK, Pasricha R, Ahmad A, Khan MI (2007) Nitrate reductase-mediated synthesis of silver nanoparticles from AgNO3. Biotechnol Lett 29:439–445

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors (Krishnendu Acharya and Dipankar Chattopadhyay) would like to thank Center for Research in Nanoscience and Nanotechnology, University of Calcutta (Sanction no. Conv/043/Nano Pr. 2009) for financial support and Anirban Chakraborty for carrying out AFM images and for AFM machine facility given by Raja Bazar Science College, University of Calcutta.

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Authors and Affiliations

  1. Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, Kolkata, 700019, India

    Joy Sarkar, Sandipan Ray & Krishnendu Acharya

  2. Department of Polymer Science and Technology, University College of Science and Technology, University of Calcutta, Kolkata, 700009, India

    Dipankar Chattopadhyay

  3. Transmission Electron Microscopy Section (Infectious Disease and Immunology), Indian Institute of Chemical Biology, Kolkata, 700032, India

    Aparna Laskar

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  1. Joy Sarkar
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  2. Sandipan Ray
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  5. Krishnendu Acharya
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Correspondence to Krishnendu Acharya.

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Sarkar, J., Ray, S., Chattopadhyay, D. et al. Mycogenesis of gold nanoparticles using a phytopathogen Alternaria alternata . Bioprocess Biosyst Eng 35, 637–643 (2012). https://doi.org/10.1007/s00449-011-0646-4

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  • DOI: https://doi.org/10.1007/s00449-011-0646-4

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