Abstract
Effects of phytohormones including indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and 1-naphthalentacetic acid (NAA) on mycelial growth of medicinal mushroom Phellinus linteus were investigated. Under the optimal IAA, IBA and NAA concentration of 1.0, 1.5 and 5.0 mg/l, the maximal mycelial diameter reached 8.6 ± 0.4, 7.3 ± 2.6 and 9.0 ± 1.0 mm, respectively. The mycelial biomass and exopolysaccharide (EPS) production with addition of 5.0 mg/l NAA in a shake flask were 6.24 ± 0.18 g/l at 168 h and 0.86 ±0.01 g/l at 192 h, which were enhanced by 15.98 and 56.36% compared to the control, respectively. However, the molecular weight and infrared spectrum of EPS were coincident with the control. Results indicated that NAA at the proper concentration was beneficial in stimulating mycelial growth and EPS biosynthesis, whereas it could not alter the molecular structure of EPS.
References
Baker JR, Kim JS, Park SY (2008) Composition and proposed structure of a water-soluble glycan from the Keumsa Sangwhang mushroom (Phellinus linteus). Fitoterapia 79:345–350
Moradali MF, Mostafavi H, Ghods S, Hedjaroude GA (2007) Immunonodulating and anticancer agents in the realm of macromycetes fungi (macrofungi). Int Immunopharmacol 7:701–724
Zhang M, Cui SW, Cheung PCK, Wang Q (2007) Antitumor polysaccharides from mushrooms: a review on their isolation process, structural characteristics and antitumor activity. Trends Food Sci Tech 18:4–19
Li G, Kim D, Kim T, Park B, Park H, Park J, Na M, Kim H, Hong N, Lim K (2004) Protein-bound polysaccharide from Phellinus linteus induces G2/M phase arrest and apoptosis in SW480 human colon cancer cells. Cancer Lett 216:175–181
Kim GY, Lee JY, Lee JO, Ryu CH, Choi BT, Jeong YK, Lee KW, Jeong SC, Choi YH (2006) Partial characterization and immunostimulatory effect of a novel polysaccharide–protein complex extracted from Phellinus linteus. Biosci Biotech Biochem 70:1218–1226
Sliva D, Kawasaki J, Stanley G, Harvey K, Slivova V (2006) Phellinus linteus inhibits growth and invasive behavior of breast cancer cells through the suppression of Akt signaling. FASEB J 20:559–560
Hwang HJ, Kim SW, Choi JW, Yun JW (2003) Production and characterization of exopolysaccharides from submerged culture of Phellinus linteus KCTC 6190. Enzyme Microb Technol 33:309–319
Zou X, Guo X, Sun M (2009) pH control strategy in a shaken minibioreactor for polysaccharide production by medicinal mushroom Phellinus linteus and its anti-hyperlipemia activity. Bioprocess Biosyst Eng 32:277–281
Tang YJ, Zhong JJ (2002) Fed-batch fermentation of Ganoderma lucidum for hyperproduction of polysaccharide and ganoderic acid. Enzyme Microb Technol 31:20–28
Otsuji K, Honda Y, Sugimura Y, Takei A, Tejima T (1995) Production of polysaccharides by plant cell culture and their applications to cosmetics. Plant Tissue Culture Lett 12:8–15
Sharma N, Chandel KPS, Srivastava VK (1991) In vitro propagation of coleus forskohlii Briq., a threatened medicinal plant. Plant Cell Rep 10:67–70
Lal N, Ahuja PS (1989) Propagation of Indian Rhubarh (Rheum emodi Wall.) using shoot-tip and leaf explant culture. Plant Cell Rep 8:493–496
Gill R, Malhotra PK, Gosal SS (2006) Direct plant regeneration from cultured young leaf segments of sugarcane. Plant Cell Tissue Organ Cult 84:100205–100209
Deng GC, Hou SM, Tian DM, Zhang YY, Zhang X, Gu JL, Zhang SL (2006) Separation and determination of selenium polysaccharide in enriched-Se Cordyceps militaris. Spectrosc Spect Anal 26:522–525
Miller GE (1959) Use of DNS a reagent for determination of reducing sugars. Anal Chem 31:426–428
Reski R (2007) Small molecules on the move: homeostasis, crosstalk, and molecularaction of phytohormones. Plant Biol 8:277–280
Kim YS, Hahn EJ, Yeung EC, Paek KY (2003) Lateral root development and saponin accumulation as affected by IBA or NAA in adventitious root cultures of Panax ginseng C.A. Meyer. In Vitro Cell Dev Biol Plant 39:245–249
Jonathan SG, Fasidi IO (2003) Studies on Psathyerella atroumbonata (Pegler), a Nigerian edible fungus. Food Chem 81:481–484
Zhong JJ (1998) Production of ginseng saponin and polysaccharide by cell cultures of Panax notoginseng and Panax ginseng. Appl Biochem Biotechnol 75:261–268
Wu MJ, Jiang DZ, Liu TM, Zhang LP (2006) Structural analysis of water-soluble polysaccharide PIP1 extracted from the cultured mycelium of Phellinus igniarius. Chem Res Chinese Univ 22:701–711
Rodriges S, Lona MFL, Franco T (2005) The effect of maltose on dextran yield and molecular weight distribution. Bioprocess Biosyst Eng 28:9–14
Shu CH, Lung MY (2004) Effect of pH on the production and molecular weight distribution of exopolysaccharide by Antrodia camphorata in batch cultures. Process Biochem 39:931–937
Acknowledgments
Financial support was received from Chongqing Natural Science Foundation Program (Contract No. CSTC, 2005BB1101), and the Opening Fund of Chongqing Key Laboratory of Luminescence and Real-time Analysis (Project No. CSTC, 2006CA8006).
Author information
Authors and Affiliations
Corresponding authors
Additional information
An erratum to this article can be found at http://dx.doi.org/10.1007/s00449-009-0344-7
Rights and permissions
About this article
Cite this article
Guo, X., Zou, X. & Sun, M. Effects of phytohormones on mycelial growth and exopolysaccharide biosynthesis of medicinal mushroom Pellinus linteus . Bioprocess Biosyst Eng 32, 701–707 (2009). https://doi.org/10.1007/s00449-009-0326-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00449-009-0326-9