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Penicillin acylase immobilization depending on macromolecular crowding and catalysis in aqueous–organic medium

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Abstract

To enhance penicillin acylase (PA) performance, it was immobilized in mesocellular silica foams (MCFs) depended on macromolecular crowding and applied to catalysis in non-aqueous medium. Ficoll 70, dextran 10,000, dextran 40,000 and bovine serum albumin were co-assembled with PA. It was observed that specific activity of PA assembled in MCFs with dextran 10,000 in 80% cyclohexane (v/v) was 233.2 U/mg, 200% as that of PA assembled in MCFs in 80% cyclohexane and 323.5% as that of free PA in full aqueous medium. As content of alkane increased, activity of PA in MCFs with macromolecules varied slightly. In addition, PA co-immobilized with dextran 10 in MCFs retained 58.2% of its initial activity after heating at 50°C for 4 h, 1.2 times higher than that of PA immobilized alone in MCFs. The results showed that macromolecular crowding was favorable for immobilization of PA and its catalysis in suitable aqueous–organic medium.

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Acknowledgments

This work was financially supported by the National High Technology Research and Development Program of China (863 Program, No. 2006AA02Z211), National Natural Science Foundation of China (20376034), Natural Science Foundation of Jiangsu Province of China (BK2006181) and Foundation of Jiangsu Province of China for College Postgraduate Students in Innovation Engineering (2007).

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Authors and Affiliations

  1. Department of Chemistry and Materials Science, Chaohu University, 238000, Chaohu, People’s Republic of China

    Jianyue Xue

  2. Research Center for Biomedicine and Health, Hangzhou Normal University, 310036, Hangzhou, People’s Republic of China

    Anming Wang

  3. National Engineering Research Center for Biotechnology, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, 210009, Nanjing, People’s Republic of China

    Anming Wang, Cheng Zhou & Shubao Shen

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  1. Jianyue Xue
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  2. Anming Wang
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Correspondence to Shubao Shen.

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Xue, J., Wang, A., Zhou, C. et al. Penicillin acylase immobilization depending on macromolecular crowding and catalysis in aqueous–organic medium. Bioprocess Biosyst Eng 32, 765–772 (2009). https://doi.org/10.1007/s00449-009-0301-5

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  • DOI: https://doi.org/10.1007/s00449-009-0301-5

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