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Expression of lymphatic endothelium-specific hyaluronan receptor LYVE-1 in the developing mouse kidney

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Abstract

Our knowledge of the embryonic development of the lymphatic vessels within the kidney is limited. The aim of this study was to establish the time of appearance and the distribution of intra-renal lymphatic vessels in the developing mouse kidney by using the lymphatic marker, LYVE-1. Kidneys from embryonic day 12 (E12) to E18, from neonates at post-natal day 1 (P1) to P21, and from adults were studied. In the adult mouse kidney, LYVE-1 was expressed mainly in the lymphatic endothelial cells (LECs) and in a subset of endothelial cells in the glomerular capillaries. However, in the developing mouse kidney, LYVE-1 was also expressed transiently in F4/80+/CD11b– immature macrophages/dendritic cells and in the developing renal vein. LYVE-1+ lymphatic vessels connected with extra-renal lymphatics were detected in the kidney at E13. F4/80+/CD11b–/LYVE-1+ immature macrophages/dendritic cells appeared prior to the appearance of LYVE-1+ renal lymphatic vessels and were closely intermingled or even formed part of the lymphatic vascular wall. Prox1 was expressed only in the LYVE-1+ LECs from fetus to adult-hood, but not in LYVE-1+ endothelial cells of the developing renal vein and macrophages/dendritic cells. Thus, lymphatic vessels of the kidney might originate by extension of extra-renal lymphatics through an active branching process possibly associated with F4/80+/CD11b–/LYVE-1+ macrophages/dendritic cells.

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Acknowledgments

The authors are grateful to Dr. Craig C. Tisher and Kirsten M. Madsen (University of Florida) for advice during this study and also thank Hong-Lim Kim for technical assistance.

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Authors and Affiliations

  1. Department of Anatomy and MRC for Cell Death Disease Research Center, College of Medicine, The Catholic University of Korea, 505, Banpo-Dong, Seocho-Ku, Seoul, 137-701, Korea

    Hyun-Wook Lee, Yu-Mi Kim, Eun-Young Park, Jin-Sun Hwang, Wan-Young Kim & Jin Kim

  2. Department of Internal Medicine and Cell Death Disease Research Center, College of Medicine, The Catholic University of Korea, Seoul, Korea

    Chul-Woo Yang

  3. Department of Histology and Embryology, Binzhou Medical University, Binzhou, China

    Yan-Xia Qin & Guan-Hua Huo

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  1. Hyun-Wook Lee
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  2. Yan-Xia Qin
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Correspondence to Jin Kim.

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Hyun-Wook Lee and Yan-Xia Qin contributed equally to this work. Some of this work has been published in abstract form (J Am Soc Nephrol 17:TH-PO644, 2006) and presented at the Renal Week 2006 Meeting, San Diego, Calif., USA, November 16–19, 2006.

This work was supported by the Korea Science and Engineering Foundation (R13-2002-005-03001-0) through the Medical Research Center for Cell Death Disease Research Center at The Catholic University of Korea.

The authors declare no conflicts of interest.

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Lee, HW., Qin, YX., Kim, YM. et al. Expression of lymphatic endothelium-specific hyaluronan receptor LYVE-1 in the developing mouse kidney. Cell Tissue Res 343, 429–444 (2011). https://doi.org/10.1007/s00441-010-1098-x

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