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Diversity of CD97 in smooth muscle cells

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Abstract

CD97, an epidermal growth factor (EGF)-TM7 receptor, is not restricted to hematopoetic and carcinoma cells but is also found on smooth muscle cells (SMC). We have examined its location and biochemical structure in various normal and tumorigenic SMC-containing tissues. SMC of the urinary bladder, lung bronchi and bronchioles, myometrium, and gastrointestinal tract were immunohistologically stained by using monoclonal antibodies (mabs) to the CD97 stalk region (CD97stalk). Mabs directed against an N-glycosylation-dependent epitope within the EGF-domains (CD97EGF) did not bind to normal SMC. Vascular SMC, which was also CD97EGF-negative, showed further CD97 heterogeneity. Only a few, if any, SMC from the aorta or elastic arteries of the systemic circulation were positive for CD97 mRNA and therefore also for CD97stalk. CD97stalk-positive SMC were slightly more numerous in muscular and peripheral arteries. In contrast, most venous SMC expressed CD97stalk. A comparison with other SMC molecules revealed a similar but not identical staining pattern for CD97stalk and desmin. Further CD97 heterogeneity was observed during SMC transformation. All leiomyomas (n=5) and nine out of 21 leiomyosarcomas were positive for both CD97stalk and CD97EGF. As expected, CD97EGF-positive SMC tumors expressed partly N-glycosylated CD97. Seven out of 21 leiomyosarcomas were completely devoid of CD97. Thus, CD97 showed variable expression in vascular and biochemical modification in tumorigenic SMC, suggesting that the function of the molecule is specific for the SMC subtype.

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Acknowledgements

We thank Dr. J. Neuhaus (Department of Urology, University of Leipzig) and Dr. M. Steinert (Städtisches Krankenhaus Martha-Maria Halle-Dölau, Halle, Germany) for providing bladder and lung tissues.

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Authors and Affiliations

  1. Department of Surgery, Faculty of Medicine Research Laboratories, Center of Surgery, University of Leipzig, Liebigstrasse 20, 04103, Leipzig, Germany

    Gabriela Aust, Elke Wandel, Doreen Sittig & Manja Wobus

  2. Department of Pathology, University of Rostock, 18055, Rostock, Germany

    Carsten Boltze

  3. Institute of Pathology, University of Leipzig, 04103, Leipzig, Germany

    Alexander Schütz & Lars-Christian Horn

Authors
  1. Gabriela Aust
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  2. Elke Wandel
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  3. Carsten Boltze
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  4. Doreen Sittig
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  5. Alexander Schütz
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  6. Lars-Christian Horn
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  7. Manja Wobus
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Corresponding author

Correspondence to Gabriela Aust.

Additional information

This study was supported by a joint grant from the German Research Council (DFG; project AU 132/3-1) and by the Interdisziplinary Center of Clinical Research (IZKF) Leipzig at the Faculty of Medicine, University of Leipzig (project D6). E. Wandel is a fellow of the IZKF.

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Aust, G., Wandel, E., Boltze, C. et al. Diversity of CD97 in smooth muscle cells. Cell Tissue Res 324, 139–147 (2006). https://doi.org/10.1007/s00441-005-0103-2

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