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Molecular cytogenetic investigation of chromosome composition and transmission in sugarcane

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Abstract

Modern sugarcane cultivars (Saccharum spp., 2n = 100–120) are complex polyploids derived from interspecific hybridization performed a century ago between the sugar-producing species S. officinarum L. and the wild species S. spontaneum L. Using genomic in situ hybridization, we revealed that between 15 and 27.5% of the genome of modern cultivars is derived from S. spontaneum, including 10–23% of entire chromosomes from this wild species and 8–13% chromosomes derived from interspecific recombination. We confirmed the occurrence of 2n + n transmission in crosses and first backcrosses between these two species and demonstrated that this also can occur in crosses between S. officinarum and modern cultivars. We analysed five S. officinarum clones with more than 80 chromosomes and demonstrated that they were derived from interspecific hybridization supporting the classical view that this species is characterized by 2n = 80. We also illustrated the complementarities between molecular cytogenetics and genetic mapping approaches for analysing complex genomes.

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Notes

  1. Q165 is protected by plant breeder’s rights in Australia.

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Acknowledgments

The authors are grateful for funding contributions from the following organizations: Australian Sugar Research and Development Corporation; Australian Academy of Science; French Embassy in Canberra; Cooperative Research Centre for Sugar Industry Innovation through Biotechnology; BSES Limited, and CIRAD. We thank J.C. Glaszmann and N. Berding for critical reading of the manuscript.

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Authors and Affiliations

  1. BSES Limited, PMB 57, Mackay, QLD, 4741, Australia

    George Piperidis & Nathalie Piperidis

  2. Cooperative Research Centre for Sugar Industry Innovation through Biotechnology, The University of Queensland, 5th Floor, John Hines Building, Brisbane, QLD, 4072, Australia

    Nathalie Piperidis

  3. CIRAD, Centre de Coopération Internationale en Recherche Agronomique pour le Développement, UMR1098/DAP, TAA96/03 avenue Agropolis, 34398, Montpellier Cedex 5, France

    Angélique D’Hont

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  1. George Piperidis
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  2. Nathalie Piperidis
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  3. Angélique D’Hont
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Correspondence to George Piperidis.

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Communicated by C. Gebhardt.

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Piperidis, G., Piperidis, N. & D’Hont, A. Molecular cytogenetic investigation of chromosome composition and transmission in sugarcane. Mol Genet Genomics 284, 65–73 (2010). https://doi.org/10.1007/s00438-010-0546-3

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