Abstract
The afp gene encoding the antifungal protein (AFP) of Aspergillus giganteus has a prototypical alkaline gene expression pattern, which suggests that the gene might be under the control of the ambient pH-dependent zinc-finger transcription factor PacC. This notion is corroborated by the presence in the upstream region of afp of two putative PacC binding sites, afpP1 and afpP2, which are specifically recognised by the PacC protein of A. nidulans in vitro. However, in this report we provide several lines of evidence to show that pH-dependent up-regulation of afp is not mediated by transcriptional activation through PacC. (1) The temporal expression pattern of the A. giganteus pacC gene does not parallel the accumulation of the afp mRNA during cultivation. (2) Inactivation of afpP1 and afpP2 did not reduce promoter activity under alkaline conditions, as determined from the relative wild-type and mutant afp::lacZ reporter activities in A. nidulans. (3) Reporter activities in acidity- and alkalinity-mimicking mutant strains are inconsistent with a positive role for PacC in afp expression. (4) In A. giganteus, the pH-dependent increase in afp mRNA and AFP levels can be completely prevented by the calcineurin inhibitor FK506, suggesting that the calcineurin signalling pathway might control the in vivo activation of the afp promoter by alkaline pH.
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Acknowledgements
We would like to thank Susanne Engelhardt, Barbara Walewska and Lily Stanton for technical assistance. We are grateful to acknowledge Eduardo Espeso and Miguel Peñalva for kindly providing us with plasmids and for helpful suggestions and discussions. Work in the Imperial College laboratory was supported by the Wellcome Trust (Grant No. 067878) and by a grant to V.M. from the Berliner Programm zur Förderung der Chancengleichheit von Frauen in Forschung und Lehre.
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Meyer, V., Spielvogel, A., Funk, L. et al. Alkaline pH-induced up-regulation of the afp gene encoding the antifungal protein (AFP) of Aspergillus giganteus is not mediated by the transcription factor PacC: possible involvement of calcineurin. Mol Genet Genomics 274, 295–306 (2005). https://doi.org/10.1007/s00438-005-0002-y
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DOI: https://doi.org/10.1007/s00438-005-0002-y