Abstract
The recessive ‘tall rice’ phenotype associated with the mutation eui (elongated upper-most internode) is an important agronomic trait that has been introduced into hybrid rice to eliminate panicle enclosure in all types of male-sterile lines and produce good-quality seeds in high yield and at low cost. Based on our previous Eui mapping data, we conducted fine-structure mapping and positional cloning of the gene using an F2 population comprising more than 5000 individuals derived from a cross of the near-isogenic lines 307T ( eui/eui) with the recurrent parent Zhenshan 97 ( Eui/Eui). In total 45 CAPS (cleaved amplified polymorphic sequences) markers located within an interval of 14.5 cM were analyzed in the subpopulation of 1298 homozygous recessive plants. The resulting high-resolution map defined a 98-kb interval containing the Eui locus flanked by the markers M0387 and M01, and three markers were found to co-segregate with Eui. In order to facilitate the identification of the Eui gene, we used a transformation-competent artificial chromosome (TAC) vector to construct a set of contiguous TAC clones from the Nipponbare BACs (obtained from the Clemson University Genome Institute; CUGI) spanning this region. These clones can be used to streamline complementation testing. The markers tightly linked to the Eui locus can also be used in breeding male-sterile lines with the elongated uppermost internode.
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Acknowledgements
We thank Bin Han for providing the rice BACs and sequence information, Rod Wing for permission to use the BACs, Dabing Zhang, Xudong Zhu and Qingyao Shu for help in growing rice. This work was funded by grants (2001AA222321 and 2002AA2Z1003) from the Ministry of Science and Technology of China and a grant (30270136) from the National Science Foundation of China grant
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Communicated by R. Hagemann
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Xu, YH., Zhu, YY., Zhou, HC. et al. Identification of a 98-kb DNA segment containing the rice Eui gene controlling uppermost internode elongation, and construction of a TAC transgene sublibrary. Mol Genet Genomics 272, 149–155 (2004). https://doi.org/10.1007/s00438-004-1019-3
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DOI: https://doi.org/10.1007/s00438-004-1019-3