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Genomic characterization of Rim2 / Hipa elements reveals a CACTA-like transposon superfamily with unique features in the rice genome

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Abstract

The availability of huge amounts of rice genome sequence now permits large-scale analysis of the structure and molecular characteristics of the previously identified transposase-encoding Rim2 (also called Hipa) element, which is transcriptionally activated by infection with the fungal pathogen Magnaporthe grisea and by treatment with the corresponding fungal elicitor. Based on genomic cloning and data mining from 230 Mb of rice genome sequence, 347 Rim2 elements, with an average size of 5.8 kb, were identified. This indicates that an estimated total of 600–700 Rim2 elements are present in the whole genome. Rim2 insertions occur non-randomly on the chromosomes, as visualized by fluorescence in situ hybridization. The elements harbor 16-bp terminal inverted repeats with the core sequence CACTG, 16-bp sub-terminal repeats, internal variable regions, 3-bp target sequence duplications in the flanking regions, and genes coding for Rim2 proteins (the putative transposase) and hydroxyproline-rich glycoproteins. High levels of insertion into genic regions are observed for members of this family, and the transposition history of the family can be deduced from the high level of shared sequences and analysis of repeat target sites of the elements. Phylogenetic analysis indicates that the putative RIM2 proteins fall into a subgroup distinct from the TNP2-like subgroup of transposases. Southern hybridization with genomic DNA from monocotyledonous and dicotyledonous plants demonstrates that the RIM2-coding sequence is unique to the Oryza genome. Our results demonstrate that the Rim2 elements from rice belong to a distinct superfamily of CACTA-like elements with evolutionary diversity.

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Acknowledgements

We thank Pamela Ronald for providing the rice BACs, and Susan R. Wessler, Thomas Bureau and Antoni Rafalski for useful comments on this work. This work was funded by NSFC grants (30125030, 90208010), a CAS grant (KSCX2-SW-301-02) and a MOST of China grant (2001AA222321) to Z.H.

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Authors and Affiliations

  1. SHARF and National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, 200032, Shanghai, China

    G.-D. Wang, G. Wu, Q. Li & Z.-H. He

  2. Biotechnology Institute, Zhejiang University, 310029, Hangzhou, China

    P.-F. Tian, D.-B. Li & Z.-H. He

  3. Department of Biology, East China Normal University, 200062, Shanghai, China

    G.-D. Wang

  4. Department of Horticulture, University of Wisconsin, Madison, WI 53706, USA

    Z.-K. Cheng & J.-M. Jiang

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  1. G.-D. Wang
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  2. P.-F. Tian
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  3. Z.-K. Cheng
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  4. G. Wu
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  5. J.-M. Jiang
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  6. D.-B. Li
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  7. Q. Li
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  8. Z.-H. He
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Correspondence to Z.-H. He.

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Communicated by M.-A. Grandbastien

The first two authors contributed equally to this work

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Wang, GD., Tian, PF., Cheng, ZK. et al. Genomic characterization of Rim2 / Hipa elements reveals a CACTA-like transposon superfamily with unique features in the rice genome. Mol Genet Genomics 270, 234–242 (2003). https://doi.org/10.1007/s00438-003-0918-z

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