Abstract
Opisthorchis viverrini infection and the subsequent bile duct cancer it induces remains a significant public health problem in Southeast Asia. Opisthorchiasis has been reported to cause reduced plasma glucose levels among infected patients. The underlying mechanism for this phenomenon is unclear. In the present study, evidence is presented to support the hypothesis that O. viverrini exploits host cholangiocyte glucose transporters (GLUTs) in a similar manner to that of rodent intestinal nematodes, to feed on unabsorbed glucose in the bile for survival. GLUT levels in a cholangiocyte H69 cell line co-cultured with excretory-secretory products of O. viverrini were examined using qPCR and immunoblotting. GLUT 8 mRNA and expressed proteins were found to be downregulated in H69 cells in the presence of O. viverrini. This suggests that O. viverrini alters glucose metabolism in cells within its vicinity by limiting transporter expression resulting in increased bile glucose that it can utilize and potentially explains the previously reported anti-insulin effect of opisthorchiasis.
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Acknowledgements
We thank all the staffs and colleagues working at the Tropical Disease Research Centre, Khon Kaen University, for their support from the very beginning until accomplishment of this project.
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This research was supported by the Fundamental Fund of Khon Kaen University and the National Science, Research and Innovation Fund (NSRF).
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S.K., S.T., B.S., and S.S. conceived and designed the study. S.K., S.C., P.S., and S.S. conducted experiments. All authors contributed to data analysis and wrote the first draft of the manuscript. S.K. and S.S. edited and finalized the manuscript.
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The protocols were approved by the KKU Animal Ethics Committee (IACUC-KKU-43/66) and KKU Human Ethics Committee (HE661490).
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Key findings
• The underlying mechanism for opisthorchiasis reduced plasma glucose levels among infected patients is unclear.
• Opisthorchis viverrini excretory-secretory products suppress GLUT8 of cholangiocytes.
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Kha, S., Chaiyadet, S., Saichua, P. et al. Opisthorchis viverrini excretory-secretory products suppress GLUT8 of cholangiocytes. Parasitol Res 123, 161 (2024). https://doi.org/10.1007/s00436-024-08184-3
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DOI: https://doi.org/10.1007/s00436-024-08184-3