Abstract
Nosema bombycis, an obligate intracellular parasite, is a single-celled eukaryote known to infect various tissues of silkworms, leading to the manifestation of pebrine. Trehalase, a glycosidase responsible for catalyzing the hydrolysis of trehalose into two glucose molecules, assumes a crucial role in thermal stress tolerance, dehydration, desiccation stress, and asexual development. Despite its recognized importance in these processes, the specific role of trehalase in N. bombycis remains uncertain. This investigation focused on exploring the functions of trehalase 3 in N. bombycis (NbTre3). Immunofluorescence analysis of mature (dormant) spores indicated that NbTre3 primarily localizes to the spore membrane or spore wall, suggesting a potential involvement in spore germination. Reverse transcription–quantitative polymerase chain reaction results indicated that the transcriptional level of NbTre3 peaked at 6 h post N. bombycis infection, potentially contributing to energy storage for proliferation. Throughout the life cycle of N. bombycis within the host cell, NbTre3 was detected in sporoplasm during the proliferative stage rather than the sporulation stage. RNA interference experiments revealed a substantial decrease in the relative transcriptional level of NbTre3, accompanied by a certain reduction in the relative transcriptional level of Nb16S rRNA. These outcomes suggest that NbTre3 may play a role in the proliferation of N. bombycis. The application of the His pull-down technique identified 28 proteins interacting with NbTre3, predominantly originating from the host silkworm. This finding implies that NbTre3 may participate in the metabolism of the host cell, potentially utilizing the host cell’s energy resources.
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We are grateful to all who provided the means for us to access free software, which we have used and cited in this article. We thank all partners and lab members for kindly help and criticism. We also thank Shanghai Applied Protein Technology Co., Ltd. for protein identification by LC-MS/MS.
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MM, ML, QH, YX, XY, BK and YZ: investigation and methodology development; MM and ML: validation and data processing; QW, XT, ZS, YZ and GZ: conceptualization and visualization; MM: writing original draft; YZ: review and editing; GZ: project administration and funding acquisition.
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Ma, M., Ling, M., Huang, Q. et al. Functional characterization of Nosema bombycis (microsporidia) trehalase 3. Parasitol Res 123, 59 (2024). https://doi.org/10.1007/s00436-023-08082-0
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DOI: https://doi.org/10.1007/s00436-023-08082-0