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Immunological characteristics of CD103+CD8+ Tc cells in the liver of C57BL/6 mouse infected with plasmodium NSM

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Abstract

CD103 is an important marker of tissue-resident memory T cells (TRM) which play important roles in fighting against infection. However, the immunological characteristics of CD103+ T cells are not thoroughly elucidated in the liver of mouse infected with Plasmodium. Six- to eight-week-old C57BL/6 mice were infected with Plasmodium yoelii nigeriensis NSM. Mice were sacrificed on 12–16 days after infection and the livers were picked out. Sections of the livers were stained, and serum aspartate aminotransferase (AST) and alanine transaminase (ALT) levels were measured. Moreover, lymphocytes in the liver were isolated, and the expression of CD103 was determined by using qPCR. The percentage of CD103 on different immune cell populations was dynamically observed by using flow cytometry (FCM). In addition, the phenotype and cytokine production characteristics of CD103+CD8+ Tc cell were analyzed by using flow cytometry, respectively. Erythrocyte stage plasmodium infection could result in severe hepatic damage, a widespread inflammatory response and the decrease of CD103 expression on hepatic immune cells. Only CD8+ Tc and γδT cells expressed higher levels of CD103 in the uninfected state.CD103 expression in CD8+ Tc cells significantly decreased after infection. Compared to that of CD103− CD8+ Tc cells, CD103+ CD8+ Tc cells from the infected mice expressed lower level of CD69, higher level of CD62L, and secreted more IL-4, IL-10, IL-17, and secreted less IFN-γ. CD103+CD8+ Tc cells might mediate the hepatic immune response by secreting IL-4, IL-10, and IL-17 except IFN-γ in the mice infected with the erythrocytic phase plasmodium, which could be involved in the pathogenesis of severe liver damage resulted from the erythrocytic phase plasmodium yoelii nigeriensis NSM infection.

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Data availability

You can email the corresponding author for more details on the method and data of this paper. The datasets used and/or analyzed during the current study available from the corresponding author on reasonable request.

Abbreviations

TRM:

Tissue-resident memory T cells

AST:

Aspartate aminotransferase

ALT:

Alanine transaminase

qPCR:

Quantitative PCR

P. falciparum :

Plasmodium falciparum

P. vivax :

Plasmodium vivax

S. japonicum :

Schistosoma japonicum

S. japonicum :

Schistosoma japonicum

PBS:

Phosphate Buffered Saline

PMA:

Phorbol 12-myristate 13-acetate

FCM:

Flow cystometry

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Funding

This project was funded by Key Discipline of Guangzhou Education Bureau (Basic Medicine) (201851839), the Natural Science Foundation of Guangdong Province (2020A1515010251, 2021A1515011032), Guangzhou Science and Technology Project (202002030082), and the Open Foundation Key Laboratory of Tropical Diseases Control (Sun Yatsen University), Ministry of Education (2021kfkt03), and Plan on enhancing scientific research in GMU (02–410-2302269XM), Guangdong Basic and Applied Basic Research Foundation (2021A1515220034), Guangdong Medical Scientific Research Foundation (B2023344).

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Author notes

  1. Feihu Shi, Shanni Tang, Dianhui Chen and Feng Mo contributed equally to this work.

Authors and Affiliations

  1. Department of Infectious Diseases, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China

    Feihu Shi, Shanni Tang, Dianhui Chen, Feng Mo & Xingfei Pan

  2. China Sino-French Hoffmann Institute, Guangzhou Medical University, Guangzhou, China

    Feihu Shi, Shanni Tang, Dianhui Chen, Feng Mo, Jiajie Li, Chao Fang, Haixia Wei, Junmin Xing, Lin Liu, Yumei Gong, Zhengrong Tan, Ziqi Zhang, Shan Zhao & Jun Huang

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  1. Feihu Shi
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Contributions

FS, SN, DC, and FM performed the in vitro cellular test. JL and CF performed histological experiment. HW conceived the study. JX and LL prepared parasite and animal. YG and ZT analyzed the results. XP, SZ, and JH contributed to the writing of the paper. All authors read and approved the final manuscript.

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Correspondence to Xingfei Pan, Shan Zhao or Jun Huang.

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This experimental study has been reviewed and approved by ethics committee of Tarbiat Modares University (IR.MODARES.REC.1398.220). All experiments were performed in accordance with relevant guidelines and regulations. All donors signed a written informed consent.

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Shi, F., Tang, S., Chen, D. et al. Immunological characteristics of CD103+CD8+ Tc cells in the liver of C57BL/6 mouse infected with plasmodium NSM. Parasitol Res 122, 2513–2524 (2023). https://doi.org/10.1007/s00436-023-07950-z

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