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Using DNA barcoding to link cystacanths and adults of the acanthocephalan Corynosoma australe of the Southeastern Pacific Ocean (off Peru coast)

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Abstract

The objective of this study is to use DNA barcoding to link cystacanths and adults belonging to the acanthocephalans Corynosoma australe found in the Southeastern Pacific Ocean off the coast central from Peru. We sampled three species of commercial fish (Paralichthys adspersus (Steindachner), Paralabrax humeralis (Valenciennes), and Cheilodactylus variegatus (Valenciennes)) and two South American sea lions, Otaria byronia, stranded on the beaches of the city of Huacho and Barranca, Lima province. A total of 509 acanthocephalan larvae were found in the body cavity of 95 fish (prevalence 54.28%, total mean intensity 8.64). A total of 127 adult worms were found in the large intestine from two South American sea lions (P= 100%, MI= 63.5). A total of 203 larvae from P. humeralis were isolates (P=65.71%; MI= 8.83; MA=5.8), 235 (P=54.29%; MI= 12.37; MA= 6.71) from C. variegatus, and 71 (P=42.86%; MI= 4.73; MA= 2.03) from P. adspersus. All adult and larval specimens were morphologically identified as C. australe. They were generated cytochrome c oxidase subunit 1 (cox1) gene sequences of specimens and were compared with available data from GenBank. Molecular phylogenetic analysis supported our morphological identification, where the Peruvian isolates formed a clade with other isolates of C. australe from other countries of the American continent. Of the sequences obtained, two haplotypes were detected and were not identical with previous reports. Based on both DNA barcoding and morphological analyses, our finding represents the first molecular data of C. australe from Peru and the report of Cheilodactylus variegatus as a new paratenic host on the central coast, extending the knowledge and distribution range of this acanthocephalan in Southeastern Pacific Ocean.

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Data availability

The obtained sequences are deposited in GenBank under the accession numbers MZ920052-MZ920067.

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Acknowledgements

The present study is supported by Faculty of Biological Sciences, National University of San Marcos, Peru. The authors extend their appreciations to members of Biomedical Sciences Research Institute, Universidad Ricardo Palma, Peru, Laboratory of Parasitology in helping to complete this study.

Funding

We want to thank UNMSM for financing the project with internal funds with R.R. 05753-R-21 and code B21102151, and the Universidad Ricardo Palma (URP) for financing.

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  1. Aarón Mondragón-Martínez

    Present address: Research and Development Department, Natural Environment, Pedro Benvenuto 381 Street, San Miguel, Lima, Peru

Authors and Affiliations

  1. Laboratory of Biology and Molecular Genetics, Faculty of Biological Sciences, Universidad Ricardo Palma, Santiago de Surco, Lima, Peru

    Aarón Mondragón-Martínez & Lidia Cruz-Neyra

  2. Laboratory of Parasitology in Wildlife and Zoonoses, Faculty of Biological Sciences, National University of San Marcos, Lima, Peru

    Aarón Mondragón-Martínez, Martín Dávila-Rios, Rosa Martínez-Rojas, Enrique García-Candela, Estrellita Rojas De-Los-Santos, Abraham Delgado-Escalante & J.R. Sanchez-Venegas

  3. Research and Development Department, Natural Environment, Pedro Benvenuto 381 Street, San Miguel, Lima, Peru

    Estrellita Rojas De-Los-Santos & Abraham Delgado-Escalante

  4. Laboratory of Parasitology, Faculty of Biological Sciences, Universidad Ricardo Palma, Santiago de Surco, Lima, Peru

    Martín Dávila-Rios, Rosa Martínez-Rojas, Juan C. Ramos Gorbeña & Miguel Dávila-Robles

  5. Instituto de Investigaciones en Ciencias Biomédicas (INICIB), Universidad Ricardo Palma, Santiago de Surco, Lima, Peru

    Eduardo A. Pulido-Murillo

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Contributions

Aarón Mondragón-Martínez (AMM), Rosa Martínez-Rojas (RMR), and Eduardo A. Pulido-Murillo (EDPM): designed the study. AMM, RMM, Martín Dávila-Rios (MDR), and Estrellita Rojas De-Los-Santos (ERS): field work. MDR, Miguel Dávila-Robles (MDR), and Juan C. Ramos-Gorbeña (JCRG): performed laboratory analyses. Lidia Cruz-Neyra (LCN), RMR, and J. R Sanchez-Venegas (JRSV): performed statistical analyses. EDPM and Enrique Garcia-Candela (EGC): performed bioinformatics analyses. AMM, EDPM, and EGC: prepared Figs. 1–3 and Tables 1–2. AMM, RMR, MDR, and LCN: wrote the first draft of the manuscript. All authors revised the manuscript. All authors read and approved the final version of the manuscript.

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Correspondence to Aarón Mondragón-Martínez.

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This study was performed in agreement with the recommendations of all applicable institutional, national, and international guidelines for the care and use of animals. The responsible conduct of the research was approved by the university council no. 0689-2020 of the “Universidad Ricardo Palma.”

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Mondragón-Martínez, A., Dávila-Rios, M., Martínez-Rojas, R. et al. Using DNA barcoding to link cystacanths and adults of the acanthocephalan Corynosoma australe of the Southeastern Pacific Ocean (off Peru coast). Parasitol Res 122, 1883–1892 (2023). https://doi.org/10.1007/s00436-023-07889-1

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