Abstract
A morphophysiological study of tubular reabsorption and mechanisms of protein endocytosis in the kidney of frogs (Rana temporaria L.) during parasitic infection was carried out. Pseudoplasmodia and spores of myxosporidia, beforehand assigned to the genus Sphaerospora, were detected in Bowman’s capsules and in the lumen of individual renal tubules by light and electron microscopy. Remarkable morphological alteration and any signs of pathology in kidney tissue related to this myxosporean infection have not been noted. At the same time, significant changes in protein reabsorption and distribution of molecular markers of endocytosis in the proximal tubule (PT) cells in infected animals were detected by immunofluorescence confocal microscopy. In lysozyme injection experiments, the endocytosed protein and megalin expression in the infected PTs were not revealed. Tubular expression of cubilin and clathrin decreased, but endosomal recycling marker Rab11 increased or remained unchanged. Thus, myxosporean infection resulted in the alterations in lysozyme uptake and expression of the main molecular determinants of endocytosis. The inhibition of receptor-mediated clathrin-dependent protein endocytosis in amphibian kidneys due to myxosporidiosis was shown for the first time. Established impairment of the endocytic process is a clear marker of tubular cell dysfunction that can be used to assess the functioning of amphibian kidneys during adaptation to adverse environmental factors.
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All data generated or analyzed during this study are available from the corresponding author upon reasonable request.
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Acknowledgements
The microscopic studies were carried out on the basis of the Center for collective use of the IEPhB RAS.
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This study was financially supported by the State assignment to Sechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences (IEPhB RAS) (no. 075–0052-22–00).
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EVS: conceptualization, immunochemistry, visualization, data analysis, writing the original draft, review end editing. NPP: methodology, statistical analysis, data analysis, review and editing. All the authors read and approved the final manuscript.
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Seliverstova, E.V., Prutskova, N.P. Renal protein reabsorption impairment related to a myxosporean infection in the grass frog (Rana temporaria L.). Parasitol Res 122, 1303–1316 (2023). https://doi.org/10.1007/s00436-023-07830-6
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DOI: https://doi.org/10.1007/s00436-023-07830-6