Abstract
Cephenemyia stimulator and Oestrus ovis are two important parasitic bot flies (Oestridae) species causing myiasis, with a potential negative impact on the welfare of the host. Using next-generation sequencing approach and bioinformatics tools, a large panel of possible microsatellites loci was obtained in both species. Primer pairs were designed for 15 selected microsatellite loci in C. stimulator and other 15 loci in O. ovis for PCR amplification. Loci amplification and analysis were performed in four populations of each species. The results demonstrated that all selected loci were polymorphic, with the number of alleles ranging from 2 to 6 per locus in C. stimulator and 3 to 13 per locus in O. ovis. This is the first time to describe these microsatellite loci for C. stimulator and O. ovis. These two sets of microsatellite markers could be further used for biogeographic and population genetics studies.
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Acknowledgements
We would like to thank the two anonymous reviewers for the comments and suggestions that have helped to improving the manuscript. This work and the research activities of GAM are backed by the Fédération Nationale des Chasseurs (France), Fédération Départementale des Chasseurs de l’Ariège and Hautes-Pyrénées (France) (project FNC-PSN-PR16-2014). This study was backed by the Fédération Nationale des Chasseurs (France) (project: FNC-PSN-PR5-2013), the National Institute for The Agriculture and Food Research and Technology (INIA), Madrid (project: FAU2008-00010-00-00) and by Jaén University (project: RFC/PP2008/UJA_68_16_22). The activities of the researchers are partially funded by the Andalusian Plan for Scientific Research (PAI) (research groups RNM-118 and BIO-220). The authors are indebted to P. Prieto, M. Sarasa, E. Faure and R. Velarde for their help in obtaining samples for this study. The research activities included in this work all comply with Andalusian and Spanish laws regarding animal experimentation and welfare.
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Aleix-Mata, G., Expósito, M., Ruiz-Ruano, F.J. et al. Development and characterization of 15 novel polymorphic microsatellite loci for two important bot flies (Diptera, Oestridae) by next-generation sequencing. Parasitol Res 119, 2829–2835 (2020). https://doi.org/10.1007/s00436-020-06824-y
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DOI: https://doi.org/10.1007/s00436-020-06824-y