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Diagnostic value of glycoprotein band patterns of three serologic enzyme-linked immunoelectrotransfer blot assays for neurocysticercosis

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Abstract

The enzyme-linked immunoelectrotransfer blot (EITB) assay to detect antibodies in serum is a complementary tool for the diagnosis of neurocysticercosis (NCC). Presence of at least one glycoprotein band corresponding to a Taenia solium (T. solium) antigen indicates a positive result; however, EITB assays have multiple glycoprotein bands, and previous work has suggested that band patterns may have additional diagnostic value. We included 58 participants with a definitive diagnosis of NCC who received care at the Instituto Nacional de Neurología y Neurocirugía in Mexico City. Three different EITB tests were applied to participants’ serum samples (LDBio, France; US Centers for Disease Control and Prevention [CDC]; and Instituto de Diagnóstico y Referencia Epidemiológicos [InDRE]). There was substantial variability in specific glycoprotein band patterns among the three assays. However, in age- and sex-adjusted logistic regression models, the number of glycoprotein bands was positively associated with the presence of vesicular extraparenchymal cysts (InDRE adjusted odds ratio [aOR] 1.60 p < 0.001; CDC aOR 6.31 p < 0.001; LDBio aOR 2.45 p < 0.001) and negatively associated with the presence of calcified parenchymal cysts (InDRE aOR 0.63 p < 0.001; CDC aOR 0.25 p < 0.001; LDBio aOR 0.44 p < 0.001). In a sensitivity analysis also adjusting for cyst count, results were similar. In all three EITB serum antibody tests, the number of glycoprotein bands consistently predicted cyst stage and location, although magnitude of effect differed.

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Acknowledgments

Dr. Teresa Garate passed away before the submission of the final version of this manuscript.

Funding

This work was partially supported by the “Programa Iberoamericano de Ciencia y Tecnología para el Desarrollo, CYTED” [211RT0432] and by the “Programa de Investigación para el Desarrollo y la Optimización de Vacunas, Inmunomoduladores y Métodos Diagnósticos del IIB” (PROVACADI, Instituto de Investigaciones Biomédicas, UNAM, México).

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Authors and Affiliations

  1. CUNY Graduate School of Public Health and Health Policy and Institute for Implementation Science in Population Health, City University of New York, New York, NY, 10027, USA

    Matthew L. Romo

  2. School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, Hong Kong SAR

    Matthew L. Romo

  3. Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico City, Mexico

    Marisela Hernández & Edda Sciutto

  4. Administración Nacional de Laboratorios e Institutos de Salud (ANLIS) “Dr. Carlos Malbrán”, Buenos Aires, Argentina

    Osvaldo-Germán Astudillo & Gustavo Diego

  5. Instituto de Diagnóstico y Referencia Epidemiológicos (InDRE), Secretaria de Salud, Mexico City, Mexico

    Jorge Luis de-la-Rosa-Arana, Antonio Meza-Lucas & Ricardo García-Rodea

  6. Unidad de Neuroinflamación, Instituto de Investigaciones Biomédicas-Universidad Nacional Autónoma de México/Instituto Nacional de Neurología y Neurocirugía/Facultad de Medicina-Universidad Nacional Autónoma de México, Mexico City, Mexico

    Andrea Toledo

  7. Instituto Gulbenkian de Ciência (IGC), 2780-156, Oeiras, Portugal

    R. Michael E. Parkhouse

  8. Instituto de Salud Carlos III, Majadahonda, Madrid, Spain

    Teresa Garate

  9. Unidad de Neuroinflamación, Instituto de Investigaciones Biomédicas-Universidad Nacional Autónoma de México/Instituto Nacional de Neurología y Neurocirugía/Facultad de Medicina-Universidad Nacional Autónoma de México, Mexico City, Mexico

    Agnès Fleury

  10. Neurocysticercosis Clinic, Instituto Nacional de Neurología y Neurocirugía, Mexico City, Mexico

    Agnès Fleury

Authors
  1. Matthew L. Romo
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  2. Marisela Hernández
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  3. Osvaldo-Germán Astudillo
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  4. Gustavo Diego
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  5. Jorge Luis de-la-Rosa-Arana
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  6. Antonio Meza-Lucas
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  7. Ricardo García-Rodea
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  8. Andrea Toledo
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  9. R. Michael E. Parkhouse
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  10. Teresa Garate
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  11. Edda Sciutto
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  12. Agnès Fleury
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Contributions

This study was designed by AF and MR and implemented by RMEP, TG, ES, MH, OGA, GD, JLRA, AML, RGR, and AT. MLR and AF analyzed and interpreted the data. MLR drafted the first version of the manuscript. All authors critically revised the manuscript for intellectual content, read, and approved the final manuscript.

Corresponding authors

Correspondence to Matthew L. Romo or Agnès Fleury.

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The authors declare that they have no conflicts of interest.

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Section Editor: Sabine Specht

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Romo, M.L., Hernández, M., Astudillo, OG. et al. Diagnostic value of glycoprotein band patterns of three serologic enzyme-linked immunoelectrotransfer blot assays for neurocysticercosis. Parasitol Res 119, 2521–2529 (2020). https://doi.org/10.1007/s00436-020-06750-z

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