Abstract
A diverse set of parasites and pathogens affects productivity and survival of Apis mellifera honeybees. In beekeeping, traditional control by antibiotics and molecules of synthesis has caused problems with contamination and resistant pathogens. In this research, different Laurus nobilis extracts are tested against the main honeybee pests through an integrated point of view. In vivo effects on bee survival are also evaluated. The ethanol extract showed minimal inhibitory concentration (MIC) values of 208 to 416 μg/mL, having the best antimicrobial effect on Paenibacillus larvae among all substances tested. Similarly, this leaf extract showed a significant antiparasitic activity on Varroa destructor, killing 50 % of mites 24 h after a 30-s exposure, and on Nosema ceranae, inhibiting the spore development in the midgut of adult bees ingesting 1 × 104 μg/mL of extract solution. Both ethanol extract and volatile extracts (essential oil, hydrolate, and its main component) did not cause lethal effects on adult honeybees. Thus, the absence of topical and oral toxicity of the ethanol extract on bees and the strong antimicrobial, microsporicidal, and miticidal effects registered in this study place this laurel extract as a promising integrated treatment of bee diseases and stimulates the search for other bioactive phytochemicals from plants.
References
Alippi M, Ringuelet J, Cerimele E, Re M, Henning C (1996) Antimicrobial activity of some essential oil against Paenibacillus larvae, the causal organism of American foulbrood disease. J Herbs Spices Med Plants 4:9–16. doi:10.1300/J044v04n02_03
Antúnez K, Harriet J, Gende L, Maggi M, Eguaras M, Zunino P (2008) Efficacy of natural propolis extract in the control of American Foulbrood. Vet Microbiol 131:324–331. doi:10.1016/j.vetmic.2008.04.011
Bidlack W (2000) Phytochemicals as bioactive agents. Technomic, Lancaster
Bogdanov S (2006) Contaminants of bee products. Apidologie 37:1–18. doi:10.1051/apido:2005043
Brenner R, Focks D, Arbogast R, Weaver D, Shuman D (1998) Practical use of spatial analysis in precision targeting for integrated pest management. Am Entomol 44(2):79–101
Cantwell G (1970) Standard methods for counting nosema spores. Am Bee J 100(6):222–223
Cushnie T, Lamb A (2005) Antimicrobial activity of flavonoids. Int J Antimicrob Agents 26:343–356. doi:10.1016/j.ijantimicag.2005.09.002
Cutler H, Cutler S (1999) Biologically active natural products: agrochemicals. CRC, London
Damiani N, Gende L, Fernández N, Fritz R, Eguaras M, Marcangeli J (2008) Actividad antimicrobiana frente a Paenibacillus larvae y toxicidad sobre Varroa destructor y Apis mellifera del aceite esencial de laurel (Laurus nobilis). 2nd Congreso Argentino de Apicultura, Mar del Plata
Damiani N, Gende L, Bailac P, Marcangeli J, Eguaras M (2009) Acaricidal and insecticidal activity of essential oils on Varroa destructor (Acari: Varroidae) and Apis mellifera (Hymenoptera: Apidae). Parasitol Res 106(1):145–152. doi:10.1007/s00436-009-1639-y
Damiani N, Fernández N, Maldonado L, Álvarez A, Eguaras M, Marcangeli J (2010) Bioactivity of propolis from different geographical origins on Varroa destructor (Acari: Varroidae). Parasitol Res 107(1):31–37. doi:10.1007/s00436-010-1829-7
Damiani N, Gende L, Maggi M, Palacios S, Marcangeli J, Eguaras M (2011) Repellent and acaricidal effects of botanical extracts on Varroa destructor. Parasitol Res 108:79–86. doi:10.1007/s00436-010-2043-3
Dhaliwal G, Arora R (2001) Role of phytochemicals in integrated pest management. In: Koul O, Dhaliwal G (eds) Phytochemical biopesticides. Harwood Academic, Amsterdam, pp 97–117
Di Leo LP, Retta D, Tkacik E, Ringuelet J, Coussioa J, van Baren C, Bandoni A (2009) Essential oil and by-products of distillation of bay leaves (Laurus nobilis L.) from Argentina. Ind Crop Prod 30(2):259–264. doi:10.1016/j.indcrop.2009.04.005
Eguaras M, Ruffinengo S (2006) Estrategias para el control de Varroa. Editorial Martin, Mar del Plata
EPPO (2010) EPPO standards PP 1/170 (4): side-effects on honeybees. EPPO Bull 40(3):313–319
Ertürk Ö (2006) Antibacterial and antifungal activity of ethanolic extracts from eleven spice plants. Biologia 61(3):275–278. doi:10.2478/s11756-006-0050-8
Evans J (2003) Diverse origins of tetracycline resistance in the honey bee bacterial pathogen Paenibacillus larvae. J Invertebr Pathol 83:46–50. doi:10.1016/S0022-2011(03)00039-9
Floris I, Carta C, Moretti M (1996) Activités in vitro de plusieurs huiles essentielles sur Bacillus larvae White et essai au rucher. Apidologie 27(2):111–119. doi:10.1051/apido:19960206
Gende L, Floris I, Fritz R, Eguaras M (2008a) Antimicrobial activity of cinnamon (Cinnamomum zeylanicum) essential oil and its main components against Paenibacillus larvae. Bull Insectol 61:1–4
Gende L, Principal J, Maggi M, Palacios S, Fritz R, Eguaras M (2008b) Melia azedarach extract and essential oils of Cinnamomun zeylanycum, Mentha piperita and Lavandula officinalis as a control of Paenibacillus larvae. Zootec Trop 26(2):151–156
Gende L, Maggi M, Van Baren C, Di Leo LA, Bandoni A, Fritz R, Eguaras M (2010) Antimicrobial and miticide activity investigation of the Eucalyptus globulus essential oil isolated from different Argentinean geographic areas. Span J Agric Res 8(3):642–650. doi:10.5424/1260
Genersch E (2010) Honey bee pathology: current threats to honey bees and beekeeping. Appl Microbiol Biotechnol 87(1):87–97. doi:10.1007/s00253-010-2573-8
Guleria S, Tiku A (2009) Botanicals in pest management: current status and future perspectives. In: Peshin R, Dhawan A (eds) Integrated pest management: innovation-development process, vol 1. Springer, Netherlands, pp 317–329. doi:10.1007/978-1-4020-8992-3_12
He X-G (2000) On-line identification of phytochemical constituents in botanical extracts by combined high-performance liquid chromatographic-diode array detection-mass spectrometric techniques. J Chromatogr A 880:203–232. doi:10.1016/S0021-9673(00)00059-5
Hokwerda H, Bos R, Tattje D, Malingre T (1982) Composition of essential oils of Laurus nobilis L. nobilis var. angustifolia and Laurus azorica. Planta Med 44:116–119. doi:10.1055/s-2007-971415
Isman M, Machial C (2006) Pesticides based on plant essential oils: from traditional practice to commercialization. In: Rai M, Carpinella M (eds) Naturally occurring bioactive compounds, 1st edn. Elsevier, Amsterdam, pp 29–44
Jacobson M (1989) Botanical pesticides, past present and future. In: Arnason J (ed) Insecticides of plant origin. Proc Am Chem Soc, Washington, pp 1–10
Jones W, Kingkorn A (2006) Extraction of plant secondary metabolites. In: Sarker S, Latif Z, Gray A (eds) Natural products isolation, methods in biotechnology, 2nd edn. Humana, New Jersey, pp 323–335
Kayser O, Kiderlen A, Croft S (2003) Natural products as antiparasitic drugs. Parasitol Res 90:S55–S62. doi:10.1007/s00436-002-0768-3
Klein A, Vaissiere B, Cane J, Steffan-Dewenter I, Cunningham S, Kremen C, Tscharntke T (2007) Importance of pollinators in changing landscape for world crops. Proc R Soc B 274:303–313. doi:10.1098/rspb.2006.3721
Lennette S, Balows R, Hansler L, Shadony E (1987) Manual de Microbiología Clínica, 4tath edn. Panamericana, Buenos Aires
Lima M, Maia I, de Sousa B, de Morais S, Freitas S (2006) Effect of stalk and leaf extracts from Euphorbiaceae species on Aedes aegypti (Diptera, Culicidae) larvae. Rev Inst Med Trop S Paulo 48:211–214. doi:10.1590/S0036-6652006000400007
Lindberg C, Melathopoulos A, Winston M (2000) Laboratory evaluation of miticides to control Varroa jacobsoni (Acari: Varroidae), a honey bee (Hymenoptera: Apidae) parasite. J Econ Entomol 93(2):189–198. doi:10.1603/0022-0493-93.2.189
Maggi M, Ruffinengo S, Damiani N, Sardella N, Eguaras M (2009) First detection of Varroa destructor resistance to coumaphos in Argentina. Exp Appl Acarol 47(4):317–320. doi:10.1007/s10493-008-9216-0
Maggi M, Ruffinengo S, Negri P, Eguaras M (2010a) Resistance phenomena to amitraz from populations of the ectoparasitic mite Varroa destructor of Argentina. Parasitol Res 107(5):1189–1192. doi:10.1007/s00436-010-1986-8
Maggi M, Ruffinengo S, Gende L, Sarlo E, Eguaras M, Bailac P, Ponzi M (2010b) Laboratory evaluations of Syzygium aromaticum (L.) Merr. et Perry essential oil against Varroa destructor. J Essent Oil Res 22(2):119–122. doi:10.1080/10412905.2010.9700278
Maggi M, Gende L, Russo K, Fritz R, Eguaras M (2011) Bioactivity of Rosmarinus officinalis essential oil extracted from plant material with different drying treatment against Paenibacillus larvae and Varroa destructor. Nat Prod Res 25(4):397–406. doi:10.1080/14786419.2010.481261
Maistrello L, Lodesani M, Costa C, Leonardi F, Marani G, Caldon M, Mutinelli F, Granato A (2008) Screening of natural compounds for the control of nosema disease in honey bees (Apis mellifera). Apidologie 39:436–444. doi:10.1051/apido:2008022
Martel A, Zeggane S, Aurieres C, Drajnudel P, Faucon J, Aubert M (2007) Acaricide residues in honey and wax after treatment of honey bee colonies with Apivar (R) or Asuntol (R) 50. Apidologie 38:534–544. doi:10.1051/apido:2007038
Medici S, Sarlo E, Porrini M, Braunstein M, Eguaras M (2012) Genetic variation and widespread dispersal of Nosema ceranae in Apis mellifera colonies of Argentina. Parasitol Res 110:859–864. doi:10.1007/s00436-011-2566-2
Mohamed A, El-Emary G, Ali H (2010) Influence of some citrus essential oils on cell viability, glutathione-S-transferase and lipid peroxidation in Ehrlich ascites carcinoma cells. J Am Sci 6(10):820–826
Morse R, Flottum K (1997) Honey bee pests, predators, and diseases. A.I. Root Company, Medina
Nanasombat S, Lohasupthawee P (2005) Antibacterial activity of crude ethanolic extracts and essential oils of spices against Salmonellae and other Enterobacteria. KMITL Sci Tech J 5(3):527–538
Ong E (2004) Extraction methods and chemical standardization of botanicals and herbal preparations. J Chromatogr B 812:23–33. doi:10.1016/j.jchromb.2004.07.041
Ozek T, Bozan B, Baser K (1998) Supercritical CO2 extraction of volatile components from leaves of Laurus nobilis L. Chem Nat Compd 34:668–671. doi:10.1007/BF02336090
Patrakar R, Mansuriya M, Patil P (2012) Phytochemical and pharmacological review on Laurus nobilis. Int J Pharm Chem Sci 1(2):595–602
Pohorecka K (2004) Laboratory studies on the effect of standardized Artemisia absinthium L. extract on Nosema apis infection in the worker Apis mellifera. J Apic Sci 48(2):131–136
Porrini M (2013) Formulaciones bacterianas y sustancias alternativas para el control de la parasitosis causada por Nosema spp. (Microspora, Nosematidae) en colonias de Apis mellifera (Hymenoptera, Apidae). PhD Thesis, Universidad Nacional de Mar del Plata, Argentina
Porrini M, Fernández N, Garrido P, Gende L, Medici S, Eguaras M (2011) In vivo evaluation of antiparasitic activity of plant extracts on Nosema ceranae (Microsporidia). Apidologie 42(6):700–707. doi:10.1007/s13592-011-0076-y
Porrini M, Garrido P, Eguaras M (2013) Individual feeding of honey bees: modification of the Rinderer technique. J Apicult Res, (in press)
Ruffinengo S, Eguaras M, Floris I, Faverin C, Bailac P, Ponzi M (2005) LD50 and repellent effects of essential oils from Argentinean wild plant species on Varroa destructor. J Econ Entomol 98:651–655. doi:10.1603/0022-0493-98.3.651
Santoyo S, Lloría R, Jaime L, Ibanez E, Senorans F, Reglero G (2006) Supercritical fluid extraction of antioxidant and antimicrobial compounds from Laurus nobilis L. chemical and functional characterization. Eur Food Res Technol 222:565–571. doi:10.1007/s00217-005-0027-9
Sarker S, Latif Z, Gray A (2006) Natural products isolation, methods in biotechnology, 2nd edn. Humana, New Jersey
Schmahl G, Al-Rasheid K, Abdel-Ghaffar F, Klimpel S, Mehlhorn H (2010) The efficacy of neem seed extracts (Tre-san®, MiteStop®) on a broad spectrum of pests and parasites. Parasitol Res 107:261–269. doi:10.1007/s00436-010-1915-x
Semmler M, Abdel-Ghaffar F, Al-Rasheid K, Mehlhorn H (2009) Nature helps: from research to products against blood-sucking arthropods. Parasitol Res 105:1483–1487. doi:10.1007/s00436-009-1634-3
Simić M, Kundaković T, Kovačević N (2003) Preliminary assay on the antioxidative activity of Laurus nobilis extracts. Fitoterapia 74:613–616. doi:10.1016/S0367-326X(03)00143-6
Simić A, Soković D, Ristić M, Grujić-Jovanović S, Vukojević J, Marin P (2004) The chemical composition of some Lauraceae essential oils and their antifungal activities. Phytother Res 18(9):713–717. doi:10.1002/ptr.1516
Umpiérrez M, Santos E, Mendoza Y, Altesor P, Rossini C (2013) Essential oil from Eupatorium buniifolium leaves as potential varroacide. Parasitol Res 112:3389–3400. doi:10.1007/s00436-013-3517-x
vanEngelsdorp D, Lengerich E, Spleen A, Dainat B, Cresswell J, Baylis K, Nguyen B, Soroker V, Underwood R, Human H, Le Conte Y, Saegerman C (2013) Standard epidemiological methods to understand and improve Apis mellifera health. In: Dietemann V, Neumann P (Eds) The COLOSS BEEBOOK: Volume II: Standard methods for Apis mellifera pest and pathogen research. J Apicult Res 52(4). doi:10.3896/IBRA.1.52.1.08
Zeković Z, Lepojević Ž, Mujić I (2009) Laurel extracts obtained by steam distillation, supercritical fluid and solvent extraction. J Nat Prod 2:104–109
Acknowledgments
We would like to thank to E. Tkacik for providing the plant material and volatile extracts. We thank Kenneth Coonrod for grammatical corrections. This study was financially supported by a CONICET grant (Innova-T Fundation) to ND, PICT Project no. 1625 (ANPCYT) to LBG, and Exa Project no. EXA579/12 (UNMDP) to ME, Argentina.
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Damiani, N., Fernández, N.J., Porrini, M.P. et al. Laurel leaf extracts for honeybee pest and disease management: antimicrobial, microsporicidal, and acaricidal activity. Parasitol Res 113, 701–709 (2014). https://doi.org/10.1007/s00436-013-3698-3
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DOI: https://doi.org/10.1007/s00436-013-3698-3