Abstract
Green nanoparticle synthesis has been achieved using environmentally acceptable plant extract and ecofriendly reducing and capping agents. The present study was carried out to establish the larvicidal activity of synthesized silver nanoparticles (AgNPs) using leaf extract of Nerium oleander (Apocynaceae) against the first to fourth instar larvae and pupae of malaria vector, Anopheles stephensi (Diptera: Culicidae). Nanoparticles are being used in many commercial applications. It was found that aqueous silver ions can be reduced by the aqueous extract of the plant parts to generate extremely stable silver nanoparticles in water. The results were recorded from UV–Vis spectrum, Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and energy-dispersive X-ray (EDX) spectroscopy analysis. The production of the AgNPs synthesized using leaf extract of N. oleander was evaluated through a UV–Vis spectrophotometer in a wavelength range of 200 to 700 nm. This revealed a peak at 440 nm in N. oleander leaf extracts, indicating the production of AgNPs. The FTIR spectra of AgNPs exhibited prominent peaks at 509.12 cm−1 (C–H bend alkenes), 1,077.05 cm−1 (C–O stretch alcohols), 1,600.63 cm−1 (N–H bend amines), 2,736.49 and 2,479.04 cm−1 (O–H stretch carboxylic acids), and 3,415.31 cm−1 (N–H stretching due to amines group). An SEM micrograph showed 20–35-nm-size aggregates of spherical- and cubic-shaped nanoparticles. EDX showed the complete chemical composition of the synthesized nanoparticles of silver. Larvicidal activity of aqueous leaf extract of N. oleander and synthesized AgNPs was carried out against Anopheles stephensi, and the results showed that the highest larval mortality was found in the synthesized AgNPs against the first to fourth instar larvae and pupae of Anopheles stephensi with the following values: LC50 of instar larvae 20.60, 24.90, 28.22, and 33.99 ppm; LC90 of instar larvae 41.62, 50.33, 57.78, and 68.41 ppm; and LC50 and LC90 of pupae 39.55 and 79.10 ppm, respectively. The aqueous leaf extract exhibited larval toxicity against the first to fourth instar larvae and pupae of Anopheles stephensi with the following values: LC50 of instar larvae 232.90, 273.71, 318.94, and 369.96 ppm; LC90 of instar larvae 455.95, 563.10, 639.86, and 730.30 ppm; and LC50 and LC90 of pupae 426.01 and 805.13 ppm, respectively. The chi-square value was significant at p < 0.05 level. The possible larvicidal activity may be due to penetration of nanoparticles through a membrane. The results could suggest that the use of plant N. oleander to synthesize silver nanoparticles is a rapid, environmentally safer, and greener approach for mosquito control. This could lead us to a new possibility in vector-control strategy.
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References
Abbott WS (1925) A method of computing the effectiveness of insecticides. J Eco Ento 18:265–267
Abhijit D (2011) Alstonia scholaris R.Br. Apocynaceae: phytochemistry and pharmacology: a concise review. J Appl Pharma Sci 01(06):51–57
Agalya Priyadarshini K, Murugan K, Panneerselvam C, Ponarulselvam S, Jiang-Shiou H, Nicoletti M (2012) Biolarvicidal and pupicidal potential of silver nanoparticles synthesized using Euphorbia hirta against Anopheles stephensi Liston (Diptera: Culicidae). Parasitol Res 111:997–1006
Ahmad A, Mukherjee P, Mandal D, Senapati S, Khan MI, Kumar R, Sastry M (2003) Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum. Colloids Surf B Biointerfaces 28:313–318
Ahmad N, Sharma S, Alam MK, Singh VN, Shamsi SF, Mehta BR, Fatma A (2010) Rapid synthesis of silver nanoparticles using dried medicinal plant of basil. Colloids Surf B Biointerfaces 81:81–86
Alder HL, Rossler EB (1977) Introduction to probability and statistics, 6th edn. Freeman, San Francisco, p 246
Amer A, Mehlhorn H (2006) Larvicidal effects of various essential oils against Aedes, Anopheles, and Culex larvae (Diptera, Culicidae). Parasitol Res 99:466–472
Anyaele OO, Amusan AAS (2003) Toxicity of hexanoic extracts of Dennettia tripetala (G. Baxer) on larvae of Aedes aegypti (L.). Afri J Biomed Res 6:49–53
Ascher KRS, Schmutterer H, Zebitz CPW, Naqvi SNH (1995) The Persian lilac or chinaberry tree: Melia azedarach L. In: Schmutterer H (ed) The neem tree: source of unique natural products for integrated pest management, medicine, industry and other purposes. VCH, Weinheim, pp 605–642
Aslani MR, Movassaghi AR, Mohri M, Abbasian A, Zarehpour M (2004) Clinical and pathological aspect of experiment oleander (Nerium oleander) toxicosis in sheep. Vet Res Commun 28(7):609–616
Benn T, Westerhoff P (2008) Nanoparticle silver released into water from commercially available sock fabrics. Environ Sci Technol 42:4133–4139
Breman JG, Martin AS, Mills A (2004) Conquering the intolerable burden of malaria: what’s new, what’s needed: a summary. AmJTrop Med Hyg 71(2):1–15
Burfield T, Reekie SL (2005) Mosquitoes, malaria and essential oils. Int J Aroma 15:30–41
Chen L, Evans JR (2009) Arched structures created by colloidal droplets as they dry. Langmuir 25:11299–11301
Cho K, Park J, Osaka T, Park S (2005) The study of antimicrobial activity and preservative effects of nanosilver ingredient. Electrochim Acta 51:956–960
Duran N, Marcato PD, Alves OL, Souza GI, Esposito E (2005) Mechanistic aspects of biosynthesis of silver nanoparticles by several Fusarium oxysporum strains. J Nanobiotechnol 13:3–8
Elechiguerra JL, Burt JL, Morones JR, Camacho-Bragado A, Gao X, Lara HH, Yacaman JM (2005) Interaction of silver nanoparticles with HIV-1. J Nanobiotechnol 29:3–6
Finney DJ (1971) Probit analysis. Cambridge University Press, London, pp 68–78
Garima Z, Amla B (2011) GC-MS analysis of the desert plants of Apocynaceae family: Nerium oleander L. and Thevetia peruviana (Pers.) Sghum. Int J Pharnac Res Develp 3(10):49–62
Huang J, Li Q, Sun D, Lu Y, Su Y, Yang X, Wang H, Wang Y, Shao W, He N, Hong J, Chen C (2007) Biosynthesis of silver and gold nanoparticles by novel sundried Cinnamomum camphora leaf. Nanotechnology 18:105104
Hynes BE, Bessen HA, Wightman WD (1985) Oleander tea: herbal draught of death. Ann Emerg Med 14:350–353
Kalyanasundaram M, Das PK (1985) Larvicidal and synergistic activity of plant extracts for mosquito control. Indian J Med Res 82:19–23
Krishnaraj C, Jagan EG, Rajasekar S, Selvakumar P, Kalaichelvan PT, Mohan N (2010) Synthesis of silver nanoparticles using Acalypha indica leaf extracts and its antibacterial activity against water borne pathogens. Colloids Surf B Biointerfaces 76:50–56
Kumar V, Yadav SK (2009) Plant-mediated synthesis of silver and gold nanoparticles and their applications. J ChemTechnol Biotechnol 84:151–157
Kumar V, Yadav SC, Yadav SK (2010) Syzygium cumini leaf and seed extract mediated biosynthesis of silver nanoparticles and their characterization. J Chem Technol & Biotech 85:1301–1309
Kundu S, Mandal M, Ghosh SK, Pal T (2004) Photochemical deposition of SERS active silver nanoparticles on silica gel. J Photochem Photobiol A Chem 162:625–663
Langford SD, Boor PJ (1996) Oleander toxicity: an examination of human and animal toxic exposures. Toxicology 109:1–13
Lokesh R, Leonard Barnabas E, Madhuri P, Saurav K, Sundar K (2010) Larvicidal activity of Trigonella foenum and Nerium oleander leaves against mosquito larvae found in Vellore City. Ind Curr Res J Biol Sci 2(3):154–160
Mahitha B, Deva Prasad Raju B, Dillip GR, Madhukar Reddy C, Mallikarjuna K, Manoj I, Priyanka S, Jayantha Rao K, John Sushma N (2011) Biosynthesis, characterization and antimicrobial studies of AgNPs extract from Bacopa monniera whole plant. Dig J Nanomat Biostruct 6(1):135–142
Marimuthu S, Rahuman AA, Rajakumar G, Santhoshkumar T, Kirthi AV, Jayaseelan C, Bagavan A, Zahir AA, Elango G, Kamaraj C (2011) Evaluation of green synthesized silver nanoparticles against parasites. Parasitol Res 10:2212–2224
Mittal PK, Adak T, Subbarao SK (2005) Inheritance of resistance to Bacillus sphaericus toxins in a laboratory selected strain of Anopheles stephensi (Diptera: Culicidae) and its response to Bacillus thuringiensis var. israelensis. Curr Sci 89:442–443
Naresh Kumar A, Murugan K, Rejeeth C, Madhiyazhagan P, Barnard DR (2011) Green synthesis of silver nanoparticles for the control of mosquito vectors of malaria, filariasis, and dengue. Vector-Borne Zoo Dis. doi:10.1089/vbz.2011.0661
Natarajan K, Selvaraj S, Murty VR (2010) Microbial production of silver nanoparticle. Digest J Nanomat and Biostruct 5:135–140
Nathan SS, Chung PG, Murugan K (2006) Combined effect of biopesticides on the digestive enzymatic profiles of Cnaphalocrocis medinalis (Guenée) (the rice leaf folder) (Insecta: Lepidoptera: Pyralidae). Ecotoxicol Environ Saf 64:82–89
Noginov MA, Zhu G, Bahoura M, Adegoke J, Small C, Ritzo BA, Drachev VP, Shalaev VM (2006) The effect of gain and absorption on surface plasmon in metal nanoparticles. Appl Phys B 86:458–460
Panneerselvam C, Ponarulselvam S, Murugan K (2011) Potential anti-plasmodial activity of synthesized silver nanoparticle using Andrographis paniculata Nees (Acanthaceae). Arch Appl Sci Res 3(6):208–217
Panneerselvam C, Murugan K, Kovendan K, Mahesh Kumar P (2012) Mosquito larvicidal, pupicidal, adulticidal, and repellent activity of Artemisia nilagirica (family: Compositae) against Anopheles stephensi and Aedes aegypti. Parasitol Res. doi:10.1007/s00436-012-3073-9
Peng Z, Beckett AN, Engler RJ, Hoffman DR, Ott NL, Simons FER (2004) Immune responses to mosquito saliva in 14 individuals with acute systemic allergic reactions to mosquito bites. J Allergy Clin Immunol 114:1189–1194
Piyarat SWK, Freed M, Roy S (1974) Biologically active plant extract for the control of mosquito larvae. Mosq News 34:398
Rajkumar G, Rahuman AA (2011) Larvicidal activity of synthesized silver nanoparticles using Eclipta prostrata leaf extract against filariasis and malaria vector. Acta Trop. doi:10.1016/j.actatropica.2011.03.003
Raut RW, Niranjan S, Kolekar Jaya R, Lakkakula Vijay D, Mendhulkar SB, Kashid (2010) Extracellular synthesis of silver nanoparticles using dried leaves of Pongamia pinnata (L) Pierre. Nano Micro Lett 2:106–113
Salunkhe RB, Patil SV, Patil CD, Salunke BK (2011) Larvicidal potential of silver nanoparticles synthesized using fungus Cochliobolus lunatus against Aedes aegypti (Linnaeus, 1762) and Anopheles stephensi Liston (Diptera; Culicidae). Parasitol Res. doi:10.1007/s00436-011-2328-10
Sap-Iam N, Homklinchan C, Larpudomlert R, Warisnoicharoen W, Sereemaspun A, Dubas ST (2010) UV irradiation induced silver nanoparticles as mosquito larvicides. J Appl Sci 10(23):3132–3136
Sathyavathi R, Balamurali Krishna M, Venugopal Rao S, Saritha R, Narayana Rao D (2010) Biosynthesis of silver nanoparticles using Coriandrum sativum leaf extract and their application in nonlinear optics. Adv Sci Lett 3:1–6
Shankar SS, Rai A, Ahmad A, Sastry M (2004) Rapid synthesis of Au, Ag, and bimetallic Au core Ag shell nanoparticles using Neem (Azadirachta indica) leaf broth. J Colloid Interface Sci 275:496–502
Shanthi R, Lakshmi G, Priyadarshini AM, Anandaraj L (2011) Phytochemical screening of Nerium oleander Linn. leaves and Momordica charantia leaves. Int Res J Pharm 2(1):131–135
Sharma SK, Upadhyay AK, Haque MA, Tyagi PK, Raghavendra K, Dash AP (2010) Wash-resistance and field evaluation of alphacypermethrin treated long-lasting insecticidal net (Interceptor) against malaria vectors Anopheles culicifacies and Anopheles fluviatilis in a tribal area of Orissa, India. Acta Trop 116(1):24–30
Shrivastava S, Dash D (2010) Label-free colorimetric estimation of proteins using nanoparticles of silver. Nano-Micro Lett 2:164–168
Snow RW, Guerra CA, Noor AM, Myint HY, Hay SI (2005) The global distribution of clinical episodes of Plasmodium falciparum malaria. Nature 434(7030):214–217
Song JY, Kim BS (2009) Rapid biological synthesis of silver nanoparticles using plant leaf extracts. Bioprocess Biosyst Eng 32:79–84
Soni N, Prakash S (2012) Efficacy of fungus mediated silver and gold nanoparticles against Aedes aegypti larvae. Parasitol Res 110:175–184
Soto-Blanco B, Fontenele-Neto JD, Silva DM, Reis PF, Nobrega JE (2006) Acute cattle intoxication from Nerium oleander pods. Trop Anim Health Prod 38:451–454
Stuart BH (2002) Polymer analysis. United Kingdom: John Wiley & Sons
Sundaravadivelan C, Nalini Padmanabhan M, Sivaprasath P, Kishmu L (2012) Biosynthesized silver nanoparticles from Pedilanthus tithymaloides leaf extract with anti-developmental activity against larval instars of Aedes aegypti L. (Diptera, Culicidae). Parasitol Res. doi:10.1007/s00436-012-3138-9
Turney K, Drake TJ, Smith JE, Tan W, Harriso WW (2004) Functionalized nanoparticles for liquid atmospheric pressure matrix-assisted laser desorption/ionization peptide analysis. Rapid Commun Mass Spectrom 18:2367–2374
Watt JM, Breyer-Brandwijk MG (1962) The medicinal and poisonous plants of Southern and Eastern Africa. E & S Livingstone Ltd., Edinburgh, pp 107–109
Wei H, Chen C, Han B, Wang E (2008) Enzyme colorimetric assay using unmodified silver nanoparticles. Anal Chem 80:7051–7055
White J (1987) Elapid snakes: Venom toxicity and action. In: Covacevich J, Davie P, Pearn J (eds) Toxic plants and animals: A guide for Australia. The Queensland Museum, Brisbane
Xu H, Käll M (2002) Morphology effects on the optical properties of silver nanoparticles. J Nanosci Nanotechnol 4:254–259
Zipcodezoo (2012) Nerium. In: Flora of China, vol. 16, p 173. Science Press (Beijing) and Missouri Botanical Garden Press
Acknowledgments
The authors would like to sincerely thank the Defence Research Development Organisation, Bharathiar University Centre for Life Science for the SEM and XRD analysis of silver nanoparticles and Dr. K. Sasikala, professor and head of the Department of Zoology, Bharathiar University, Coimbatore-641 046, India, for the laboratory facilities provided.
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Roni, M., Murugan, K., Panneerselvam, C. et al. Evaluation of leaf aqueous extract and synthesized silver nanoparticles using Nerium oleander against Anopheles stephensi (Diptera: Culicidae). Parasitol Res 112, 981–990 (2013). https://doi.org/10.1007/s00436-012-3220-3
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DOI: https://doi.org/10.1007/s00436-012-3220-3