Skip to main content

Advertisement

SpringerLink
Log in

Molecular characterization of Cryptosporidium in children in Oyo State, Nigeria: implications for infection sources

  • Short Communication
  • Published:
Parasitology Research Aims and scope Submit manuscript

Abstract

A study was conducted to detect and identify Cryptosporidium spp. in 43 children from Oyo State, Nigeria. Using nested polymerase chain reaction, 11.6% of the children were identified as positive for Cryptosporidium spp. Restriction fragment length polymorphism analysis and DNA sequencing of the PCR products showed the presence of three subtype families of Cryptosporidium hominis (two isolates of Ia and one isolate of Ib) and Cryptosporidium parvum (two isolates of IIc), all anthroponotic in nature. This study identified a high diversity of Cryptosporidium subtypes and clearly suggested that anthroponotic rather than zoonotic transmission played a more important role in the epidemiology of Cryptosporidium in the studied area.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Alves M, Xiao L, Sulaiman IM, Lal AA, Matos O, Antunes F (2003) Subgenotype analysis of Cryptosporidium isolates from humans, cattle, and zoo ruminants in Portugal. J Clin Microbiol 41:2744–2747

    Article  PubMed  CAS  Google Scholar 

  • Cama VA, Ross JM, Crawford S, Kawai V, Chavez-Valdez R, Vargas D, Vivar A, Ticona E, Navincopa M, Williamson J, Ortega Y, Gilman RH, Bern C, Xiao L (2003) Differences in clinical manifestations among Cryptosporidium species and subtypes in HIV-infected persons. J Infect Dis 169(5):684–691

    Google Scholar 

  • Cama VA, Bern C, Roberts J, Cabrera L, Sterling CR, Ortega Y, Gilman RH, Xiao L (2008) Cryptosporidium species and subtypes and clinical manifestations in children, Peru. Emerg Infect Dis 14:1567–1574

    Article  PubMed  Google Scholar 

  • Hunter PR, Thompson RC (2005) The zoonotic transmission of Giardia and Cryptosporidium. Int J Parasitol 35:1181–1190

    Article  PubMed  Google Scholar 

  • Mallon ME, MacLeod A, Wastling JM, Smith H, Tait A (2003) Multilocus genotyping of Cryptosporidium parvum type 2: population genetics and sub-structuring. Infect Genet Evol 3:207–218

    Article  PubMed  CAS  Google Scholar 

  • Molloy SF, Smith HV, Kirwan P, Nichols RAB, Asaolu SO, Connelly L, Holland CV (2010) Identification of a high diversity of Cryptosporidium species genotypes and subtypes in a pediatric population in Nigeria. Am J Trop Med Hyg 82:608–613

    Article  PubMed  Google Scholar 

  • Sulaiman IM, Hira PR, Zhou L, Al-Ali FM, Al-Shelahi FA, Shweiki HM (2005) Unique endemicity of cryptosporidiosis in children in Kuwait. J Clin Microbiol 43:2805–2809

    Article  PubMed  Google Scholar 

  • Xiao L (2010) Molecular epidemiology of cryptosporidiosis: an update. Experimental Parasitol 124:80–89

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by the John D. and Catherine T. MacArthur Foundation Overseas Training Grant from the University of Ibadan (Nigeria) and the Centers for Disease Control and Prevention, Atlanta, GA, USA. We thank Theresa Dearen for technical assistance. This study received approval from the University of Ibadan/University College Hospital Ethics Committee with the assigned number UI/EC/10/0129. The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the Centers for Disease Control and Prevention.

Author information

Authors and Affiliations

  1. Department of Veterinary Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria

    Adekunle Bamidele Ayinmode & Benjamin Olakunle Fagbemi

  2. Division of Foodborne, Waterborne, and Environmental Diseases, Centers for Disease Control and Prevention, Atlanta, GA, 30333, USA

    Adekunle Bamidele Ayinmode & Lihua Xiao

Authors
  1. Adekunle Bamidele Ayinmode
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Benjamin Olakunle Fagbemi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lihua Xiao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Adekunle Bamidele Ayinmode.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ayinmode, A.B., Fagbemi, B.O. & Xiao, L. Molecular characterization of Cryptosporidium in children in Oyo State, Nigeria: implications for infection sources. Parasitol Res 110, 479–481 (2012). https://doi.org/10.1007/s00436-011-2531-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00436-011-2531-0

Keywords

Search

Navigation