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Leishmania mexicana promastigotes secrete a protein tyrosine phosphatase

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Abstract

Leishmania mexicana is an intracellular protozoan parasite that infects macrophages and dendritic cells and causes a chronic cutaneous disease. Although many enzymatic activities have been reported in this parasite, the presence of kinases and phosphatases has been poorly studied. These enzymes control the phosphorylation and dephosphorylation of proteins. Specifically, protein tyrosine kinases phosphorylate tyrosine residues and protein tyrosine phosphatases (PTPases) dephosphorylate tyrosine residues. PTPase activities have been reported as pathogenic factors in various infectious microorganisms such as viruses, bacteria, and parasites. Also, it has been shown that the induction of one or more PTPase activities in macrophages represents an important pathogenicity factor in Leishmania. Recently, we reported a membrane-bound PTPase activity in promastigotes of Leishmania major. In the present work, we give evidence that promastigotes of L. mexicana are able to secrete a PTPase into the culture medium. Two antibodies: one monoclonal against the catalytic domains of the human placental PTPase 1B and a polyclonal rabbit anti-recombinant protein Petase7 from Trypanosoma brucei cross-reacted with a 50-kDa molecule. The anti-human PTPase 1B antibody depleted the enzymatic activity present in the conditioned medium. The pattern of sensitivity and resistance to specific PTPase and serine/threonine inhibitors showed that this enzyme is a protein tyrosine phosphatase.

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Acknowledgements

We thank José Delgado Domínguez, Joselín Hernández, and Marco Gudiño for their excellent technical assistance; Norbert Bakalara for the gift of antibodies polyclonal rabbit anti-recombinant protein Petase 7 from T. brucei. This work was supported by grants IN221606, IN212409 from DGAPA-UNAM, and 45052-M from CONACyT. Escalona-Montaño AR was recipient of a CONACyT fellowship and is indebted to Posgrado en Ciencias Biológicas, Facultad de Ciencias, Universidad Nacional Autónoma de México.

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Authors and Affiliations

  1. Departamento de Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, Dr. Balmis 148, Colonia Doctores, México D.F., 06726, México

    Alma R. Escalona-Montaño, Daniel Pardavé-Alejandre, Rocely Cervantes-Sarabia, Laila Gutiérrez-Kobeh, Ingeborg Becker-Fauser & Maria M. Aguirre-García

  2. Laboratorio de Farmacología, Subdirección de Investigación Básica, Instituto Nacional de Cancerología, México D.F., México

    Patricia García-López

  3. Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, México D.F., México

    Manuel Gutiérrez-Quiroz

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  1. Alma R. Escalona-Montaño
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  2. Daniel Pardavé-Alejandre
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  3. Rocely Cervantes-Sarabia
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  4. Patricia García-López
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  8. Maria M. Aguirre-García
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Correspondence to Maria M. Aguirre-García.

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Escalona-Montaño, A.R., Pardavé-Alejandre, D., Cervantes-Sarabia, R. et al. Leishmania mexicana promastigotes secrete a protein tyrosine phosphatase. Parasitol Res 107, 309–315 (2010). https://doi.org/10.1007/s00436-010-1863-5

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  • DOI: https://doi.org/10.1007/s00436-010-1863-5

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