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Molecular characterization of Cryptosporidium isolates from human and bovine using 18s rRNA gene in Shahriar county of Tehran, Iran

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Abstract

Cryptosporidiosis is a widespread cause of diarrheal diseases of humans, young calves, and many mammals. Although in recent years molecular investigations on Cryptosporidium have increased, no data are available on Iran in this regard. Two species of Cryptosporidium are known to infect human beings; Cryptosporidium hominis which shows anthroponotic transmission among humans and Cryptosporidium parvum which shows zoonotic transmission between animals and humans. Cryptosporidium oocysts, morphologically identified as C. parvum, were isolated from 24 human and 35 bovine cases in Shahriar (suburb of Tehran, Iran), and genotyped by means of a Nested-polymerase chain reaction/restriction fragment length polymorphism analysis of the 18s rRNA gene. The isolates from bovine samples gave zoonotic or 2 genotype (C. parvum), and DNA profiles of human isolates gave two distinct genotypes, namely an anthroponotic or 1 (C. hominis) and zoonotic genotype or 2 (C. parvum).

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Acknowledgements

This study was funded by Tarbiat Modares University. The authors wish to thank Dr. Ghafari, Dr. Sharifi, and the personnel of Imam Sadjad and Tamin Ejtemaei Hospitals and the Veterinary Organization of Tehran for their assistance.

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Authors and Affiliations

  1. Parasitology Department, School of Medical Science, Tarbiat Modares University, Tehran, Iran

    Majid Pirestani, Javid Sadraei, Abdolhossein Dalimi asl, Mahdi Zavvar & Hossein Vaeznia

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  1. Majid Pirestani
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  2. Javid Sadraei
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  3. Abdolhossein Dalimi asl
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  4. Mahdi Zavvar
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  5. Hossein Vaeznia
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Correspondence to Javid Sadraei.

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Pirestani, M., Sadraei, J., Dalimi asl, A. et al. Molecular characterization of Cryptosporidium isolates from human and bovine using 18s rRNA gene in Shahriar county of Tehran, Iran. Parasitol Res 103, 467–472 (2008). https://doi.org/10.1007/s00436-008-1008-2

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  • DOI: https://doi.org/10.1007/s00436-008-1008-2

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