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Acrolichanus auriculatus (Digenea, Allocreadiidae): distribution of sensory papillae, musculature and FMRFamide-like immunoreactivity in adult worms

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Abstract

The muscle system and the FMRFamide immunopositive components of the nervous system of flatworms Acrolichanus auriculatus (Digenea, Allocreadiidae), an intestine parasite of acipenserid fishes, were studied for the first time by immunocytochemical, histochemical methods and confocal laser scanning microscopy. Scanning electron microscopy in A. auriculatus revealed a consistent pattern in the number and arrangement of ciliated and unciliated sensory papillae along the digenean body. Morphological data demonstrated the presence of the circular, longitudinal and diagonal muscles in different body regions of A. auriculatus. The musculature of the oral and ventral suckers, the digestive system, as well as the muscles extending radially from the genital pore are described. The study revealed the presence of the FMRFamide immunopositive staining in the central and peripheral nervous systems of A. auriculatus: in the neurons and neurites of the brain, brain commissure, in three pairs of the longitudinal nerve cords and commissures. FMRFamide immunopositive nerve elements innervate the attachment organs and the compartments of the digestive, reproductive and excretory systems.

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The data presented in this study can be available upon personal request to the corresponding authors.

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Acknowledgements

Authors wish to thank Yashin VA and Kuchin AV for technical assistance at the Optical Microscopy and Spectrophotometry Core Facilities of Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences” (Pushchino, Moscow Region).

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The authors did not receive support from any organization for the submitted work.

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  1. Natalia D. Kreshchenko and Nadezhda B. Terenina have contributed equally to this work and should both be considered as first authors.

Authors and Affiliations

  1. Institute of Cell Biophysics of Russian Academy of Sciences, Institutskaya Str., 3, 142290, Pushchino, Moscow Region, Russia

    Natalia D. Kreshchenko & Grigorii V. Kuznetsov

  2. Center of Parasitology, A.N. Severtsov Institute of Ecology and Evolution of Russian Academy of Sciences, Leninsky Prosp., 33, 119071, Moscow, Russia

    Nadezhda B. Terenina, Ekaterina L. Voropaeva, Natalia V. Mochalova & Sergey O. Movsesyan

  3. Papanin Institute for Biology of Inland Waters, Russian Academy of Sciences, 152742, Borok, Yaroslavl Province, Russia

    Larisa G. Poddubnaya

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  1. Natalia D. Kreshchenko
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  2. Nadezhda B. Terenina
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  3. Larisa G. Poddubnaya
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Contributions

Natalia D. Kreshchenko: investigation; supervision; visualization; writing-original draft, English translation; Nadezhda B. Terenina: data curation; supervision; validation; writing-original draft; Larisa G. Poddubnaya: SEM investigation; methodology, writing-original draft; Ekaterina L. Voropaeva: methodology; resources; Natalia V. Mochalova: investigation; methodology; Grigorii V. Kuznetsov: investigation, microscopy; Sergey O. Movsesyan: conceptualization; methodology; writing-original draft. All authors reviewed the manuscript.

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Correspondence to Natalia D. Kreshchenko.

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Kreshchenko, N.D., Terenina, N.B., Poddubnaya, L.G. et al. Acrolichanus auriculatus (Digenea, Allocreadiidae): distribution of sensory papillae, musculature and FMRFamide-like immunoreactivity in adult worms. Zoomorphology (2024). https://doi.org/10.1007/s00435-024-00665-4

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