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Effect of glutathione-modulating compounds on hydrogen-peroxide-induced cytotoxicity in human glioblastoma and glioma cell lines

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Abstract

The relation between the intracellular glutathione (GSH) concentration and hydrogen-peroxide(H2O2)-induced cytotoxicity was investigated. The intracellular GSH concentration in human glioblastoma (T98G, U87MG) and glioma (KG1C) cell lines was one or two orders of magnitude higher than that in a human myelogenous leukemic cell line (HL-60), which showed higher sensitivity to H2O2. Pretreatment of these cell lines with l-buthionine-[S,R]-sulfoximine, which significantly reduced the intracellular GSH concentration, increased their sensitivity against H2O2, whereas pretreatment with N-acetyl-l-cysteine, which did not significantly change the intracellular GSH concentration, only marginally protected the cells from the cytotoxic effect of H2O2. The results suggest that drug sensitivity of tumor cells can be modified by glutathione-modulating compounds.

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Authors and Affiliations

  1. Department of Neurosurgery, School of Medicine, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142, Japan Tel.: (+81) 03 3784 8605; Fax: (+81) 03 3784 8432, , , , , , JP

    Masataka Iida, Shigeki Sunaga, Nobuo Hirota & Kiyoshi Matsumoto

  2. Department of Biochemistry, School of Medicine, Showa University, Tokyo, Japan, , , , , , JP

    Nobuyuki Kuribayashi, Hiroshi Sakagami & Minoru Takeda

Authors
  1. Masataka Iida
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  2. Shigeki Sunaga
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  3. Nobuo Hirota
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  4. Nobuyuki Kuribayashi
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  5. Hiroshi Sakagami
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  6. Minoru Takeda
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  7. Kiyoshi Matsumoto
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Additional information

Received: 5 March 1997 / Accepted: 20 August 1997

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Iida, M., Sunaga, S., Hirota, N. et al. Effect of glutathione-modulating compounds on hydrogen-peroxide-induced cytotoxicity in human glioblastoma and glioma cell lines. J Cancer Res Clin Oncol 123, 619–622 (1997). https://doi.org/10.1007/s004320050115

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  • DOI: https://doi.org/10.1007/s004320050115

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