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Preliminary study of differentiating smears from cancerous and non-cancerous nasopharyngeal tissue using confocal Raman spectroscopy

  • Original Article – Clinical Oncology
  • Published:
Journal of Cancer Research and Clinical Oncology Aims and scope Submit manuscript

Abstract

Purpose

Current practice for diagnosing nasopharyngeal carcinoma (NPC) is based on invasive tissue biopsy. This study aims to explore the feasibility of using Raman spectroscopy to differentiate cancerous and non-cancerous nasopharyngeal tissue smears, expecting to realize minimal invasive diagnosis using smears from in vivo mucosa tissue by Raman spectroscopy.

Methods

Biopsy tissue smears were acquired from 74 patients with pathologically diagnosed nasopharyngeal diseases and measured using confocal Raman spectroscopy.

Results

Both fingerprint region and high wavenumber Raman spectra were acquired with distinguish features. Multivariate statistical analysis was used to differentiate cancerous and non-cancerous groups, achieving a diagnostic sensitivity of 87.2 and specificity of 85.7 % for differentiating NPC from nasopharyngeal non-cancerous smears.

Conclusions

This work indicates that the method has a unique advantage in microanalysis for tissue smears which may provide a promising minimal invasive (or noninvasive) diagnosing tool for cancer diagnosis.

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Acknowledgments

This work was supported by the Program for Changjiang Scholars and Innovative Research Team in University (IRT1115) and the National Natural Science Foundation of China (Nos. 61210016, 61178090, 61178083, and 81301253), National Natural Science Foundation of Fujian China (No. 2014J01227), the Project of Science Foundation of Ministry of Health and United Fujian Provincial Health and Education Project for Tackling the Key Research (No. WKJ-FJ-01), the Project of Fujian Provincial Education Department (No. JB13022), the Science and Technology Planning Project of Fuzhou (NO. 2011-S-66-5), Project Supported by the Natural Science Foundation of Fujian Province (2015J01499) and the Canadian Institutes of Health Research International Scientific Exchange Program.

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    Authors and Affiliations

    1. Affiliated Fuzhou First Hospital of Fujian Medical University, Fuzhou, 350009, China

      Liqing Sun, Xinheng Lin & Fengyu Zhu

    2. Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou, 350007, China

      Zhihong Xu, Wei Huang, Shanshan Wu, Nengrong Liu & Rong Chen

    3. Fujian Metrology Institute, Fuzhou, 350003, China

      Wei Huang

    4. Institute of Optical Engineering, Department of Physics, Shanghai Jiao Tong University, Shanghai, 200240, China

      Meizhen Huang

    5. Imaging Unit - Integrative Oncology Department, British Columbia Cancer Agency Research Centre, 675 West 10th Avenue, Vancouver, BC, V5Z 1L3, Canada

      Haishan Zeng

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    1. Liqing Sun
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    Correspondence to Wei Huang.

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    Liqing Sun and Zhihong Xu have contributed equally to this work.

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    Sun, L., Xu, Z., Huang, W. et al. Preliminary study of differentiating smears from cancerous and non-cancerous nasopharyngeal tissue using confocal Raman spectroscopy. J Cancer Res Clin Oncol 142, 823–831 (2016). https://doi.org/10.1007/s00432-015-2082-3

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    • DOI: https://doi.org/10.1007/s00432-015-2082-3

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