Abstract
Malformation during cortical development can disrupt the balance of excitatory and inhibitory neural circuits, contributing to various psychiatric and developmental disorders. One of the critical factors of cortical neural networks is the fine regulation of neurogenesis through mechanical cues, such as shear stress and substrate stiffness. Piezo1, a mechanically-activated channel, serves as a transducer for these mechanical cues, regulating embryogenesis. However, specific cell-type expression patterns of this channel during cortical development have not yet been characterized. In the present study, we conducted an RNAscope experiment to visualize the location of Piezo1 transcripts with embryonic neuronal/glial lineage cell markers. Our analysis covered coronal sections of the mouse forebrain on embryonic day 12.5 (E12.5), E14.5, E16.5, and E18.5. In addition, applying Yoda1, a specific Piezo1 agonist, evoked distinct calcium elevation in piriform cortices of E16.5 and E18.5 embryonic slices. Furthermore, pharmacological activation or inhibition of this channel significantly modulated the migration of neurosphere-derived cells in vitro. These findings contribute valuable insights to the field of mechanobiology and provide an understanding of the intricate processes underlying embryonic brain development.
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All original data in this study are available from the corresponding author on request.
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Acknowledgements
This research was supported by the National Research Foundation (NRF) of Korea (NRF-2020R1C1C1010245, NRF-2022M3E5E8017395, and NRF-RS-2023-00254795) grant funded by the Korean government (MSIT) and KIST Institutional Program (2E32231 and 2E31502).
Funding
The project was supported by the National Research Foundation (NRF) of Korea grants NRF-2020R1C1C1010245, NRF-2022M3E5E8017395, and NRF-RS-2023-00254795, funded by the Korean government (MSIT). Additionally, the work was supported by the Institutional Program of Korea Institute of Science and Technology, with grant numbers 2E32231 and 2E31502.
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Conceptualization: Gyu-Sang Hong; Methodology: Hye Yoon Kim, Pa Reum Lee, Bokeum Kang, and Kyungmin Kim; Formal analysis and investigation: Hye Yoon Kim, Pa Reum Lee, Bokeum Kang, and Kyungmin Kim; Writing—original draft preparation: Hye Yoon Kim and Gyu-Sang Hong; Writing—review and editing, Funding acquisition, Resources, Supervision: Gyu-Sang Hong.
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All experimental procedures were approved by the Institutional Animal Care and Use Committee of the Korea Institute of Science and Technology (KIST) (Project number: KIST-2020–112).
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Kim, H.Y., Kang, B., Lee, P.R. et al. Expression patterns of Piezo1 in the developing mouse forebrain. Brain Struct Funct 229, 759–773 (2024). https://doi.org/10.1007/s00429-024-02759-7
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DOI: https://doi.org/10.1007/s00429-024-02759-7