Abstract
Main Conclusion
The pUceS8.3 is a constitutive gene promoter with potential for ectopic and strong genes overexpression or active biomolecules in plant tissues attacked by pests, including nematode-induced giant cells or galls.
Abstract
Soybean (Glycine max) is one of the most important agricultural commodities worldwide and a major protein and oil source. Herein, we identified the soybean ubiquitin-conjugating (E2) enzyme gene (GmUBC4; Glyma.18G216000), which is significantly upregulated in response to Anticarsia gemmatalis attack and Meloidogyne incognita-induced galls during plant parasitism by plant nematode. The GmUBC4 promoter sequence and its different modules were functionally characterized in silico and in planta using transgenic Arabidopsis thaliana and G. max lines. Its full-length transcriptional regulatory region (promoter and 5´-UTR sequences, named pUceS8.3 promoter) was able to drive higher levels of uidA (β-glucuronidase) gene expression in different tissues of transgenic A. thaliana lines compared to its three shortened modules and the p35SdAMV promoter. Notably, higher β-glucuronidase (GUS) enzymatic activity was shown in M. incognita-induced giant cells when the full pUceS8.3 promoter drove the expression of this reporter gene. Furthermore, nematode-specific dsRNA molecules were successfully overexpressed under the control of the pUceS8.3 promoter in transgenic soybean lines. The RNAi gene construct used here was designed to post-transcriptionally downregulate the previously characterized pre-mRNA splicing factor genes from Heterodera glycines and M. incognita. A total of six transgenic soybean lines containing RNAi gene construct were selected for molecular characterization after infection with M. incognita pre-parasitic second-stage (ppJ2) nematodes. A strong reduction in the egg number produced by M. incognita after parasitism was observed in those transgenic soybean lines, ranging from 71 to 92% compared to wild-type control plants. The present data demonstrated that pUceS8.3 is a gene promoter capable of effectively driving dsRNA overexpression in nematode-induced giant cells of transgenic soybean lines and can be successfully applied as an important biotechnological asset to generate transgenic crops with improved resistance to root-knot nematodes as well as other pests.
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Data availability
The datasets associated with transcript abundance analyzed during the current study are available in the last release of soybean genome assembly (Glycine max Wm82.a4.v1) deposited in Phytozome 13 repository (https://phytozome-next.jgi.doe.gov/) and JGI Data Portal (https://data.jgi.doe.gov/).
Abbreviations
- DAI:
-
Days after inoculation
- GUS:
-
β-Glucuronidase enzyme
- NBTs:
-
New biotechnological tools
- ppJ2:
-
Pre-parasitic second-stage juvenile
- RKN:
-
Root-knot nematode
- UBC:
-
Ubiquitin conjugating
- uidA :
-
GUS-encoding gene
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Acknowledgements
We thank Waldir Pereira Dias from Embrapa Soybean for supplying M. incognita isolates, Regina Carneiro from Embrapa Genetic Resources and Biotechnology for her advice on the nematology bioassay, and the team at the Biological Control Lab at Embrapa Genetic Resources and Biotechnology for supplying the A. gemmatalis insects. We are also thankful to all our collaborators from INRA Sophia-Antipolis, France, especially those on the Nematology and Microscopy team.
Funding
This work was supported by the Brazilian Agricultural Research Corporation (Embrapa, call MP2, grant# 02.12.01.002.000), National Council for Scientific and Technological Development (CNPq, call PVE/Linha2, grant 401733/2013–0), Federal District Research Foundation (FAP-DF, call 03/2015 DE, grant 0193.000850/2015), INCT-PlantStress Biotech, Coordination for the Improvement of Higher Education Personnel (CAPES), ABRAPA, and UCB.
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Fragoso, R.R., Arraes, F.B.M., Lourenço-Tessutti, I.T. et al. Functional characterization of the pUceS8.3 promoter and its potential use for ectopic gene overexpression. Planta 256, 69 (2022). https://doi.org/10.1007/s00425-022-03980-6
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DOI: https://doi.org/10.1007/s00425-022-03980-6